spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bejarano, L. A.
Right arrow Articles by Gonzalez, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bejarano, L. A.
Right arrow Articles by Gonzalez, C.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Journal of Cell Science, Vol 112, Issue 23 4207-4211, Copyright © 1999 by Company of Biologists

JOURNAL ARTICLES

Motif trap: a rapid method to clone motifs that can target proteins to defined subcellular localisations

LA Bejarano and C Gonzalez
Cell Biology and Biophysics Programme, European Molecular Biology Laboratory, Heidelberg, Germany.

We have developed a rapid procedure termed Motif Trap (MT) to identify protein motifs that are able to target proteins to a distinct subcellular localisation in eukaryotic cells. By expressing random DNA fragments fused to green fluorescent protein (GFP), individual cells with the GFP localisation of interest are readily isolated allowing for the expressed DNA fragments to be cloned by RT-PCR. These can then be used to identify the corresponding full-length cDNAs. Using MT, we have identified patterns of GFP localisation which correspond to every major organelle and compartment. We have shown that MT is useful to identify new sequences that determine subcellular localisation as well as known targeting motifs.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Hum Mol GenetHome page
H. G.E. Sutherland, G. K. Mumford, K. Newton, L. V. Ford, R. Farrall, G. Dellaire, J. F. Caceres, and W. A. Bickmore
Large-scale identification of mammalian proteins localized to nuclear sub-compartments
Hum. Mol. Genet., September 1, 2001; 10(18): 1995 - 2011.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. R. Hanson and R. H. Kohler
GFP imaging: methodology and application to investigate cellular compartmentation in plants
J. Exp. Bot., April 1, 2001; 52(356): 529 - 539.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 1999