Journal of Cell Science, Vol 112, Issue 23 4223-4232, Copyright © 1999 by Company of Biologists

JOURNAL ARTICLES

Relationship between Na(+),K(+)-ATPase and cell attachment

RG Contreras, L Shoshani, C Flores-Maldonado, A Lazaro and M Cereijido
Center for Research and Advanced Studies of Mexico (CINVESTAV), Av. Instituto Politecnico Nacional 2508, Codigo Postal, 07660 Mexico.

A prolonged ouabain blockade of the Na(+),K(+)-ATPase detaches cells from each other and from the substrate. This suggests the existence of a link between pump (P) and attachment (A). In the present work, we report that MDCK-W cells treated with ouabain increase tyrosine phosphorylation and content of active MAP kinase, redistribute molecules involved in cell attachment (occludin, ZO-1, desmoplakin, cytokeratin, alpha-actinin, vinculin and actin), and detach. Genistein and UO126, inhibitors of protein tyrosine kinase and of MAP kinase kinase, respectively, block this detachment. The content of P190(Rho-GAP), a GTPase activating protein of the Rho small G-protein subfamily, is increased by ouabain, suggesting that both the Rho/Rac and MAPK pathways are involved. Another clone of MDCK cells whose Na(+),K(+)-ATPase has a negligible affinity for the drug, show none of the effects described for MDCK-W and remain attached. Ma104 cells, a line that has a high affinity for ouabain and stops pumping, fail to modify phosphorylation, as well as the pattern of distribution of attaching molecules, and remain in the monolayer. Taken together, these results suggest that there is a mechanism (P-->A) that transduces a blockade of the pump in a detachment of the cell from neighbors and substrate, in which Ma104 cells are faulty.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				T. Krupinski and G. J. Beitel Unexpected Roles of the Na-K-ATPase and Other Ion Transporters in Cell Junctions and Tubulogenesis Physiology, June 1, 2009; 24(3): 192 - 201. [Abstract] [Full Text] [PDF]

				W M Dismuke, C C Mbadugha, D Faison, and D Z Ellis Ouabain-induced changes in aqueous humour outflow facility and trabecular meshwork cytoskeleton Br J Ophthalmol, January 1, 2009; 93(1): 104 - 109. [Abstract] [Full Text] [PDF]

				W. Schoner and G. Scheiner-Bobis Endogenous and exogenous cardiac glycosides: their roles in hypertension, salt metabolism, and cell growth Am J Physiol Cell Physiol, August 1, 2007; 293(2): C509 - C536. [Abstract] [Full Text] [PDF]

				S. M. Paul, M. J. Palladino, and G. J. Beitel A pump-independent function of the Na,K-ATPase is required for epithelial junction function and tracheal tube-size control Development, January 1, 2007; 134(1): 147 - 155. [Abstract] [Full Text] [PDF]

				O. Vagin, E. Tokhtaeva, and G. Sachs The Role of the beta1 Subunit of the Na,K-ATPase and Its Glycosylation in Cell-Cell Adhesion J. Biol. Chem., December 22, 2006; 281(51): 39573 - 39587. [Abstract] [Full Text] [PDF]

				S. Bibert, S. Roy, D. Schaer, E. Felley-Bosco, and K. Geering Structural and Functional Properties of Two Human FXYD3 (Mat-8) Isoforms J. Biol. Chem., December 22, 2006; 281(51): 39142 - 39151. [Abstract] [Full Text] [PDF]

				S. J. Khundmiri, M. A. Metzler, M. Ameen, V. Amin, M. J. Rane, and N. A. Delamere Ouabain induces cell proliferation through calcium-dependent phosphorylation of Akt (protein kinase B) in opossum kidney proximal tubule cells Am J Physiol Cell Physiol, December 1, 2006; 291(6): C1247 - C1257. [Abstract] [Full Text] [PDF]

				N. Charoenphandhu, K. Tudpor, N. Pulsook, and N. Krishnamra Chronic metabolic acidosis stimulated transcellular and solvent drag-induced calcium transport in the duodenum of female rats Am J Physiol Gastrointest Liver Physiol, September 1, 2006; 291(3): G446 - G455. [Abstract] [Full Text] [PDF]

				H.-J. Park, S. M. Ward, J. S. Desgrosellier, S. P. Georgescu, A. G. Papageorge, X. Zhuang, J. V. Barnett, and J. B. Galper Transforming Growth Factor beta Regulates the Expression of the M2 Muscarinic Receptor in Atrial Myocytes via an Effect on RhoA and p190RhoGAP J. Biol. Chem., July 21, 2006; 281(29): 19995 - 20002. [Abstract] [Full Text] [PDF]

				I. Larre, A. Ponce, R. Fiorentino, L. Shoshani, R. G. Contreras, and M. Cereijido Contacts and cooperation between cells depend on the hormone ouabain PNAS, July 18, 2006; 103(29): 10911 - 10916. [Abstract] [Full Text] [PDF]

				K. Tudpor, N. Charoenphandhu, W. Saengamnart, and N. Krishnamra Long-Term Prolactin Exposure Differentially Stimulated the Transcellular and Solvent Drag-Induced Calcium Transport in the Duodenum of Ovariectomized Rats Experimental Biology and Medicine, December 1, 2005; 230(11): 836 - 844. [Abstract] [Full Text] [PDF]

				F. Lefranc, J. Brotchi, and R. Kiss Possible Future Issues in the Treatment of Glioblastomas: Special Emphasis on Cell Migration and the Resistance of Migrating Glioblastoma Cells to Apoptosis J. Clin. Oncol., April 1, 2005; 23(10): 2411 - 2422. [Abstract] [Full Text] [PDF]

				L. Shoshani, R. G. Contreras, M. L. Roldan, J. Moreno, A. Lazaro, M. S. Balda, K. Matter, and M. Cereijido The Polarized Expression of Na+,K+-ATPase in Epithelia Depends on the Association between {beta}-Subunits Located in Neighboring Cells Mol. Biol. Cell, March 1, 2005; 16(3): 1071 - 1081. [Abstract] [Full Text] [PDF]

				O. A. Akimova, A. Y. Bagrov, O. D. Lopina, A. V. Kamernitsky, J. Tremblay, P. Hamet, and S. N. Orlov Cardiotonic Steroids Differentially Affect Intracellular Na+ and [Na+]i/[K+]i-independent Signaling in C7-MDCK Cells J. Biol. Chem., January 7, 2005; 280(1): 832 - 839. [Abstract] [Full Text] [PDF]

				M. Cereijido, R. G. Contreras, and L. Shoshani Cell Adhesion, Polarity, and Epithelia in the Dawn of Metazoans Physiol Rev, October 1, 2004; 84(4): 1229 - 1262. [Abstract] [Full Text] [PDF]

				S. Yuan and E. M. Joseph The small heart Mutation Reveals Novel Roles of Na+/K+-ATPase in Maintaining Ventricular Cardiomyocyte Morphology and Viability in Zebrafish Circ. Res., September 17, 2004; 95(6): 595 - 603. [Abstract] [Full Text] [PDF]

				A. K. Rajasekaran and S. A. Rajasekaran Role of Na-K-ATPase in the assembly of tight junctions Am J Physiol Renal Physiol, September 1, 2003; 285(3): F388 - F396. [Abstract] [Full Text] [PDF]

				Z. Xie and T. Cai Na+-K+-ATPase-Mediated Signal Transduction: From Protein Interaction to Cellular Function Mol. Interv., May 1, 2003; 3(3): 157 - 168. [Abstract] [Full Text] [PDF]

				S. Taurin, N. O Dulin, D. Pchejetski, R. Grygorczyk, J. Tremblay, P. Hamet, and S. N Orlov c-Fos expression in ouabain-treated vascular smooth muscle cells from rat aorta: evidence for an intracellular-sodium-mediated, calcium-independent mechanism J. Physiol., September 15, 2002; 543(3): 835 - 847. [Abstract] [Full Text] [PDF]

				A. Y. Xiao, L. Wei, S. Xia, S. Rothman, and S. P. Yu Ionic Mechanism of Ouabain-Induced Concurrent Apoptosis and Necrosis in Individual Cultured Cortical Neurons J. Neurosci., February 15, 2002; 22(4): 1350 - 1362. [Abstract] [Full Text] [PDF]

				R. Belusa, O. Aizman, R. M. Andersson, and A. Aperia Changes in Na+-K+-ATPase activity influence cell attachment to fibronectin Am J Physiol Cell Physiol, February 1, 2002; 282(2): C302 - C309. [Abstract] [Full Text] [PDF]

				S. A. Rajasekaran, L. G. Palmer, S. Y. Moon, A. Peralta Soler, G. L. Apodaca, J. F. Harper, Y. Zheng, and A. K. Rajasekaran Na,K-ATPase Activity Is Required for Formation of Tight Junctions, Desmosomes, and Induction of Polarity in Epithelial Cells Mol. Biol. Cell, December 1, 2001; 12(12): 3717 - 3732. [Abstract] [Full Text] [PDF]

				F. Brouillard, D. Tondelier, A. Edelman, and M. Baudouin-Legros Drug Resistance Induced by Ouabain via the Stimulation of MDR1 Gene Expression in Human Carcinomatous Pulmonary Cells Cancer Res., February 1, 2001; 61(4): 1693 - 1698. [Abstract] [Full Text]