|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 112, Issue 23 4271-4279, Copyright © 1999 by Company of Biologists
JOURNAL ARTICLES |
R Milner, X Huang, J Wu, S Nishimura, R Pytela, D Sheppard and C ffrench-Constant
Wellcome/CRC Institute of Developmental Biology and Cancer, Tennis Court Road, Cambridge CB2 1QR, UK.
The alphav integrins are likely to be an important group of molecules for regulating astrocyte behaviour within the central nervous system. Together with their ligand vitronectin, they are expressed by astrocytes in vivo and are further upregulated during neurological disease. Here we have characterised the expression of alphav integrins on primary astrocytes from both rat and mouse, and shown that they express just two members, alphavbeta5 and alphavbeta8. By using RGD peptides and function-blocking antibodies against the beta1 integrins and alphavbeta5, we find that both alphavbeta5 and alphavbeta8 can act as functional receptors for vitronectin. However, while alphavbeta5 is largely responsible for astrocyte adhesion to vitronectin this integrin appears to play no role in migration on vitronectin, with alphavbeta8 playing the dominant role in promoting migration on this substrate. beta1 integrins are not involved in mediating interactions between astrocytes and vitronectin. These results were confirmed in experiments with astrocytes derived from mice in which the beta5 gene had been deleted by homologous recombination. beta5 null astrocytes attached to vitronectin at a reduced rate, but showed no defect in migration on vitronectin relative to wild-type astrocytes. These data provide the first evidence that alphavbeta8 regulates migration and show that astrocyte alphavbeta5 and alphavbeta8 integrins have distinct functions.
This article has been cited by other articles:
|
|
 |
|
  R. Milner, S. Hung, X. Wang, G. I. Berg, M. Spatz, and G. J. del Zoppo Responses of Endothelial Cell and Astrocyte Matrix-Integrin Receptors to Ischemia Mimic Those Observed in the Neurovascular Unit Stroke, January 1, 2008; 39(1): 191 - 197. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. J. del Zoppo, R. Milner, T. Mabuchi, S. Hung, X. Wang, G. I. Berg, and J. A. Koziol Microglial Activation and Matrix Protease Generation During Focal Cerebral Ischemia Stroke, February 1, 2007; 38(2): 646 - 651. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Renault-Mihara, F. Beuvon, X. Iturrioz, B. Canton, S. De Bouard, N. Leonard, S. Mouhamad, A. Sharif, J. W. Ramos, M.-P. Junier, et al. Phosphoprotein Enriched in Astrocytes-15 kDa Expression Inhibits Astrocyte Migration by a Protein Kinase C{delta}-dependent Mechanism Mol. Biol. Cell, December 1, 2006; 17(12): 5141 - 5152. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. J. del Zoppo and R. Milner Integrin-Matrix Interactions in the Cerebral Microvasculature Arterioscler. Thromb. Vasc. Biol., September 1, 2006; 26(9): 1966 - 1975. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Riemenschneider, W. Mueller, R. A. Betensky, G. Mohapatra, and D. N. Louis In Situ Analysis of Integrin and Growth Factor Receptor Signaling Pathways in Human Glioblastomas Suggests Overlapping Relationships with Focal Adhesion Kinase Activation Am. J. Pathol., November 1, 2005; 167(5): 1379 - 1387. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. H. McCarty, A. A. Cook, and R. O. Hynes An interaction between {alpha}v{beta}8 integrin and Band 4.1B via a highly conserved region of the Band 4.1 C-terminal domain PNAS, September 20, 2005; 102(38): 13479 - 13483. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. H. McCarty, A. Lacy-Hulbert, A. Charest, R. T. Bronson, D. Crowley, D. Housman, J. Savill, J. Roes, and R. O. Hynes Selective ablation of {alpha}v integrins in the central nervous system leads to cerebral hemorrhage, seizures, axonal degeneration and premature death Development, January 1, 2005; 132(1): 165 - 176. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. J. Tom, C. M. Doller, A. T. Malouf, and J. Silver Astrocyte-Associated Fibronectin Is Critical for Axonal Regeneration in Adult White Matter J. Neurosci., October 20, 2004; 24(42): 9282 - 9290. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Milner and I. L. Campbell The Extracellular Matrix and Cytokines Regulate Microglial Integrin Expression and Activation J. Immunol., April 1, 2003; 170(7): 3850 - 3858. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Jarad, B. Wang, S. Khan, J. DeVore, H. Miao, K. Wu, S. L. Nishimura, B. A. Wible, M. Konieczkowski, J. R. Sedor, et al. Fas Activation Induces Renal Tubular Epithelial Cell beta 8 Integrin Expression and Function in the Absence of Apoptosis J. Biol. Chem., November 27, 2002; 277(49): 47826 - 47833. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. H. McCarty, R. A. Monahan-Earley, L. F. Brown, M. Keller, H. Gerhardt, K. Rubin, M. Shani, H. F. Dvorak, H. Wolburg, B. L. Bader, et al. Defective Associations between Blood Vessels and Brain Parenchyma Lead to Cerebral Hemorrhage in Mice Lacking {alpha}v Integrins Mol. Cell. Biol., November 1, 2002; 22(21): 7667 - 7677. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Milner and I. L. Campbell Cytokines Regulate Microglial Adhesion to Laminin and Astrocyte Extracellular Matrix via Protein Kinase C-Dependent Activation of the alpha 6beta 1 Integrin J. Neurosci., March 1, 2002; 22(5): 1562 - 1572. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. M. Grzeszkiewicz, D. J. Kirschling, N. Chen, and L. F. Lau CYR61 Stimulates Human Skin Fibroblast Migration through Integrin alpha vbeta 5 and Enhances Mitogenesis through Integrin alpha vbeta 3, Independent of Its Carboxyl-terminal Domain J. Biol. Chem., June 8, 2001; 276(24): 21943 - 21950. [Abstract] [Full Text] [PDF]
|
|
