|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 112, Issue 24 4773-4783, Copyright © 1999 by Company of Biologists
JOURNAL ARTICLES |
M Trischler, W Stoorvogel and O Ullrich
Institut f]ur Biochemie, Universit]at Mainz, Becherweg 30, D-55128 Mainz, Germany.
Rab GTPases are associated with distinct cellular compartments and function as specific regulators of intracellular transport. In the endocytic pathway, it is well documented that Rab5 regulates transport from plasma membrane to early (sorting) endosomes. In contrast, little is known about the precise localization and function of Rab4 and Rab11, which are believed to control endocytic recycling. In the present study we have analysed the protein composition of Rab5- and Rab11-carrying endosomes to gain further insight into the compartmental organization of the endocytic and recycling pathway. Endosome populations of this transport route were purified by immunoadsorption from endosome-enriched subcellular fractions using antibodies directed against the cytoplasmic tail of the transferrin receptor, Rab5 or Rab11. Endocytosed transferrin moved sequentially through compartments that could be immunoadsorbed with anti-Rab5 and anti-Rab11, consistent with the theory that Rab5 and Rab11 localise to sorting and recycling endosomes, respectively. These compartments exhibited morphological differences, as determined by electron microscopy. Although their overall protein compositions were very similar, some proteins were found to be selectively enriched. While Rab4 was present on all endosome populations, Rab5 and Rab11 were strikingly segregated. Furthermore, the Rab11-positive endosomes were rich in annexin II, actin and the t-SNARE syntaxin 13, compared to Rab5-containing endosomes. In an in vitro assay, the Rab5 effector protein EEA1 was preferentially recruited by Rab5-positive endosomes. Taken together, our data suggest an organization of the transferrin pathway into distinct Rab5- and Rab11-positive compartments.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  Y.-C. Hsiao, Z. J. Tong, J. E. Westfall, J. G. Ault, P. S. Page-McCaw, and R. J. Ferland Ahi1, whose human ortholog is mutated in Joubert syndrome, is required for Rab8a localization, ciliogenesis and vesicle trafficking Hum. Mol. Genet., October 15, 2009; 18(20): 3926 - 3941. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. R. Silvis, C. A. Bertrand, N. Ameen, F. Golin-Bisello, M. B. Butterworth, R. A. Frizzell, and N. A. Bradbury Rab11b Regulates the Apical Recycling of the Cystic Fibrosis Transmembrane Conductance Regulator in Polarized Intestinal Epithelial Cells Mol. Biol. Cell, April 15, 2009; 20(8): 2337 - 2350. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Hayes and S. E. Moss Annexin 2 Has a Dual Role as Regulator and Effector of v-Src in Cell Transformation J. Biol. Chem., April 10, 2009; 284(15): 10202 - 10210. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Leonard, A. Hayakawa, D. Lawe, D. Lambright, K. D. Bellve, C. Standley, L. M. Lifshitz, K. E. Fogarty, and S. Corvera Sorting of EGF and transferrin at the plasma membrane and by cargo-specific signaling to EEA1-enriched endosomes J. Cell Sci., October 15, 2008; 121(20): 3445 - 3458. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Norouziyan, W.-C. Shen, and S. F. Hamm-Alvarez Tyrphostin A8 stimulates a novel trafficking pathway of apically endocytosed transferrin through Rab11-enriched compartments in Caco-2 cells Am J Physiol Cell Physiol, January 1, 2008; 294(1): C7 - C21. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Calzolari, C. Raggi, S. Deaglio, N. M. Sposi, M. Stafsnes, K. Fecchi, I. Parolini, F. Malavasi, C. Peschle, M. Sargiacomo, et al. TfR2 localizes in lipid raft domains and is released in exosomes to activate signal transduction along the MAPK pathway J. Cell Sci., November 1, 2006; 119(21): 4486 - 4498. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. L. Prigozhina and C. M. Waterman-Storer Decreased polarity and increased random motility in PtK1 epithelial cells correlate with inhibition of endosomal recycling. J. Cell Sci., September 1, 2006; 119(Pt 17): 3571 - 3582. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. L. Grosshans, D. Ortiz, and P. Novick Rabs and their effectors: Achieving specificity in membrane traffic PNAS, August 8, 2006; 103(32): 11821 - 11827. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Razi and C. E. Futter Distinct Roles for Tsg101 and Hrs in Multivesicular Body Formation and Inward Vesiculation Mol. Biol. Cell, August 1, 2006; 17(8): 3469 - 3483. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. F. J. van de Graaf, J. G. J. Hoenderop, and R. J. M. Bindels Regulation of TRPV5 and TRPV6 by associated proteins Am J Physiol Renal Physiol, June 1, 2006; 290(6): F1295 - F1302. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Li, M. M. Harraz, W. Zhou, L. N. Zhang, W. Ding, Y. Zhang, T. Eggleston, C. Yeaman, B. Banfi, and J. F. Engelhardt Nox2 and Rac1 Regulate H2O2-Dependent Recruitment of TRAF6 to Endosomal Interleukin-1 Receptor Complexes Mol. Cell. Biol., January 1, 2006; 26(1): 140 - 154. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Strick and L. A. Elferink Rab15 Effector Protein: A Novel Protein for Receptor Recycling from the Endocytic Recycling Compartment Mol. Biol. Cell, December 1, 2005; 16(12): 5699 - 5709. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Larance, G. Ramm, J. Stockli, E. M. van Dam, S. Winata, V. Wasinger, F. Simpson, M. Graham, J. R. Junutula, M. Guilhaus, et al. Characterization of the Role of the Rab GTPase-activating Protein AS160 in Insulin-regulated GLUT4 Trafficking J. Biol. Chem., November 11, 2005; 280(45): 37803 - 37813. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. A. Gomez and J. L. Daniotti H-Ras Dynamically Interacts with Recycling Endosomes in CHO-K1 Cells: INVOLVEMENT OF Rab5 AND Rab11 IN THE TRAFFICKING OF H-Ras TO THIS PERICENTRIOLAR ENDOCYTIC COMPARTMENT J. Biol. Chem., October 14, 2005; 280(41): 34997 - 35010. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. Smith, J. T. Cirulis, J. E. Casanova, M. A. Scidmore, and J. H. Brumell Interaction of the Salmonella-containing Vacuole with the Endocytic Recycling System J. Biol. Chem., July 1, 2005; 280(26): 24634 - 24641. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Bouley, H. Y. Lin, M. K. Raychowdhury, V. Marshansky, D. Brown, and D. A. Ausiello Downregulation of the vasopressin type 2 receptor after vasopressin-induced internalization: involvement of a lysosomal degradation pathway Am J Physiol Cell Physiol, June 1, 2005; 288(6): C1390 - C1401. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Yan, W. Sun, P. Kujala, Y. Lotfi, T. A. Vida, and A. J. Bean CART: An Hrs/Actinin-4/BERP/Myosin V Protein Complex Required for Efficient Receptor Recycling Mol. Biol. Cell, May 1, 2005; 16(5): 2470 - 2482. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Aschenbrenner, S. N. Naccache, and T. Hasson Uncoated Endocytic Vesicles Require the Unconventional Myosin, Myo6, for Rapid Transport through Actin Barriers Mol. Biol. Cell, May 1, 2004; 15(5): 2253 - 2263. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. B. Dale, J. L. Seachrist, A. V. Babwah, and S. S. G. Ferguson Regulation of Angiotensin II Type 1A Receptor Intracellular Retention, Degradation, and Recycling by Rab5, Rab7, and Rab11 GTPases J. Biol. Chem., March 26, 2004; 279(13): 13110 - 13118. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Fouraux, M. Deneka, V. Ivan, A. van der Heijden, J. Raymackers, D. van Suylekom, W. J. van Venrooij, P. van der Sluijs, and G. J.M. Pruijn rabip4' Is an Effector of rab5 and rab4 and Regulates Transport through Early Endosomes Mol. Biol. Cell, February 1, 2004; 15(2): 611 - 624. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Singh, D. Charkowicz, and D. Mascarenhas Insulin-like Growth Factor-independent Effects Mediated by a C-terminal Metal-binding Domain of Insulin-like Growth Factor Binding Protein-3 J. Biol. Chem., January 2, 2004; 279(1): 477 - 487. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Nagai, T. Meerloo, T. Takeda, and M. G. Farquhar The Adaptor Protein ARH Escorts Megalin to and through Endosomes Mol. Biol. Cell, December 1, 2003; 14(12): 4984 - 4996. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Haberman, E. Grimberg, M. Fukuda, and R. Sagi-Eisenberg Synaptotagmin IX, a possible linker between the perinuclear endocytic recycling compartment and the microtubules J. Cell Sci., November 1, 2003; 116(21): 4307 - 4318. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Sun, Q. Yan, T. A. Vida, and A. J. Bean Hrs regulates early endosome fusion by inhibiting formation of an endosomal SNARE complex J. Cell Biol., July 7, 2003; 162(1): 125 - 137. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Bananis, J. W. Murray, R. J. Stockert, P. Satir, and A. W. Wolkoff Regulation of early endocytic vesicle motility and fission in a reconstituted system J. Cell Sci., July 1, 2003; 116(13): 2749 - 2761. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Aschenbrenner, T. Lee, and T. Hasson Myo6 Facilitates the Translocation of Endocytic Vesicles from Cell Peripheries Mol. Biol. Cell, July 1, 2003; 14(7): 2728 - 2743. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Roosterman, F. Schmidlin, and N. W. Bunnett Rab5a and rab11a mediate agonist-induced trafficking of protease-activated receptor 2 Am J Physiol Cell Physiol, May 1, 2003; 284(5): C1319 - C1329. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Grimberg, Z. Peng, I. Hammel, and R. Sagi-Eisenberg Synaptotagmin III is a critical factor for the formation of the perinuclear endocytic recycling compartment and determination of secretory granules size J. Cell Sci., January 1, 2003; 116(1): 145 - 154. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. M. van Dam, T. ten Broeke, K. Jansen, P. Spijkers, and W. Stoorvogel Endocytosed Transferrin Receptors Recycle via Distinct Dynamin and Phosphatidylinositol 3-Kinase-dependent Pathways J. Biol. Chem., December 6, 2002; 277(50): 48876 - 48883. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Strick, D. M. Francescutti, Y. Zhao, and L. A. Elferink Mammalian Suppressor of Sec4 Modulates the Inhibitory Effect of Rab15 during Early Endocytosis J. Biol. Chem., August 30, 2002; 277(36): 32722 - 32729. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Lindsay and M. W. McCaffrey Rab11-FIP2 Functions in Transferrin Recycling and Associates with Endosomal Membranes via Its COOH-terminal Domain J. Biol. Chem., July 19, 2002; 277(30): 27193 - 27199. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Zeigerer, M. A. Lampson, O. Karylowski, D. D. Sabatini, M. Adesnik, M. Ren, and T. E. McGraw GLUT4 Retention in Adipocytes Requires Two Intracellular Insulin-regulated Transport Steps Mol. Biol. Cell, July 1, 2002; 13(7): 2421 - 2435. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Hunyady, A. J. Baukal, Z. Gaborik, J. A. Olivares-Reyes, M. Bor, M. Szaszak, R. Lodge, K. J. Catt, and T. Balla Differential PI 3-kinase dependence of early and late phases of recycling of the internalized AT1 angiotensin receptor J. Cell Biol., June 24, 2002; 157(7): 1211 - 1222. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Steiner, J.-C. F. Sarria, L. Glauser, S. Magnin, S. Catsicas, and H. Hirling Modulation of receptor cycling by neuron-enriched endosomal protein of 21 kD J. Cell Biol., June 24, 2002; 157(7): 1197 - 1209. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Gerke and S. E. Moss Annexins: From Structure to Function Physiol Rev, April 1, 2002; 82(2): 331 - 371. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Lindsay, A. G. Hendrick, G. Cantalupo, F. Senic-Matuglia, B. Goud, C. Bucci, and M. W. McCaffrey Rab Coupling Protein (RCP), a Novel Rab4 and Rab11 Effector Protein J. Biol. Chem., March 29, 2002; 277(14): 12190 - 12199. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Sheff, L. Pelletier, C. B. O'Connell, G. Warren, and I. Mellman Transferrin receptor recycling in the absence of perinuclear recycling endosomes J. Cell Biol., March 4, 2002; 156(5): 797 - 804. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Peng, E. Grimberg, and R. Sagi-Eisenberg Suppression of Synaptotagmin II restrains phorbolester-induced downregulation of protein kinase C{alpha} by diverting the kinase from a degradative pathway to the recycling endocytic compartment J. Cell Sci., January 8, 2002; 115(15): 3083 - 3092. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. de Wit, Y. Lichtenstein, R. B. Kelly, H. J. Geuze, J. Klumperman, and P. van der Sluijs Rab4 Regulates Formation of Synaptic-like Microvesicles from Early Endosomes in PC12 Cells Mol. Biol. Cell, November 1, 2001; 12(11): 3703 - 3715. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Fratti, J. M. Backer, J. Gruenberg, S. Corvera, and V. Deretic Role of phosphatidylinositol 3-kinase and Rab5 effectors in phagosomal biogenesis and mycobacterial phagosome maturation arrest J. Cell Biol., August 6, 2001; 154(3): 631 - 644. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Gagescu, N. Demaurex, R. G. Parton, W. Hunziker, L. A. Huber, and J. Gruenberg The Recycling Endosome of Madin-Darby Canine Kidney Cells Is a Mildly Acidic Compartment Rich in Raft Components Mol. Biol. Cell, August 1, 2000; 11(8): 2775 - 2791. [Abstract] [Full Text]
|
|
|
|
|
|
B. Sonnichsen, S. De Renzis, E. Nielsen, J. Rietdorf, and M. Zerial Distinct Membrane Domains on Endosomes in the Recycling Pathway Visualized by Multicolor Imaging of Rab4, Rab5, and Rab11 J. Cell Biol., May 15, 2000; 149(4): 901 - 914. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Verdile, R. N. Martins, M. Duthie, E. Holmes, P. H. St George-Hyslop, and P. E. Fraser Inhibiting Amyloid Precursor Protein C-terminal Cleavage Promotes an Interaction with Presenilin 1 J. Biol. Chem., June 30, 2000; 275(27): 20794 - 20798. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Innamorati, C. Le Gouill, M. Balamotis, and M. Birnbaumer The Long and the Short Cycle. ALTERNATIVE INTRACELLULAR ROUTES FOR TRAFFICKING OF G-PROTEIN-COUPLED RECEPTORS J. Biol. Chem., April 13, 2001; 276(16): 13096 - 13103. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Schmidlin, O. Dery, K. O. DeFea, L. Slice, S. Patierno, C. Sternini, E. F. Grady, and N. W. Bunnett Dynamin and Rab5a-dependent Trafficking and Signaling of the Neurokinin 1 Receptor J. Biol. Chem., June 29, 2001; 276(27): 25427 - 25437. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Cormont, M. Mari, A. Galmiche, P. Hofman, and Y. Le Marchand-Brustel A FYVE-finger-containing protein, Rabip4, is a Rab4 effector involved in early endosomal traffic PNAS, February 13, 2001; 98(4): 1637 - 1642. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Sheff, L. Pelletier, C. B. O'Connell, G. Warren, and I. Mellman Transferrin receptor recycling in the absence of perinuclear recycling endosomes J. Cell Biol., March 4, 2002; 156(5): 797 - 804. [Abstract] [Full Text] [PDF]
|
|
