spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nelson, P. J.
Right arrow Articles by Gelman, I. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nelson, P. J.
Right arrow Articles by Gelman, I. H.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Journal of Cell Science, Vol 112, Issue 3 361-370, Copyright © 1999 by Company of Biologists

JOURNAL ARTICLES

Involvement of the protein kinase C substrate, SSeCKS, in the actin-based stellate morphology of mesangial cells

PJ Nelson, K Moissoglu, J Vargas, PE Klotman and IH Gelman
Department of Microbiology and Division of Nephrology, Mount Sinai School of Medicine, New York, NY 10029-6574, USA.

Activation of protein kinase C is a key signal transduction event in mesangial cell dedifferentiation and proliferation, yet little is known about downstream substrates or their roles in normal or diseased glomeruli. SSeCKS, a novel protein kinase C substrate originally isolated as a src-suppressed negative mitogenic regulator in fibroblasts, controls actin-based cytoskeletal architecture and scaffolds key signaling kinases such as protein kinase C and protein kinase A. Based on the morphologic similarity between SSeCKS-overexpressing fibroblasts and stellate mesangial cells, we hypothesized that SSeCKS might play a role in mesangial cell morphology in a protein kinase C-dependent manner. Immunoblotting, in situ staining and northern blotting detected abundant expression of SSeCKS in human and rodent mesangial cells and glomerular parietal cells but not in renal tubular epithelia. Immunofluorescence analysis showed enrichment of SSeCKS in mesangial cell podosomes and along a cytoskeletal network distinct from F-actin. Activation of protein kinase C by phorbol ester resulted in a rapid serine phosphorylation of SSeCKS and its subsequent translocation to perinuclear sites, coincident with the retraction of stellate processes. These effects were blocked by concentrations of bis-indolylmaleimide that selectively inhibit protein kinase C. Finally, ablation of SSeCKS expression using retroviral anti-sense vectors induced (1) an elongated, fibroblastic cell morphology, (2) production of thick, longitudinal stress fibers and (3) repositioning of vinculin-associated focal complexes away from the cell edges. These data suggest a role for SSeCKS as a downstream mediator of protein kinase C-controlled, actin-based mesangial cell cytoskeletal architecture.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
B. Su, Y. Bu, D. Engelberg, and I. H. Gelman
SSeCKS/Gravin/AKAP12 Inhibits Cancer Cell Invasiveness and Chemotaxis by Suppressing a Protein Kinase C- Raf/MEK/ERK Pathway
J. Biol. Chem., February 12, 2010; 285(7): 4578 - 4586.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
S. Akakura, C. Huang, P. J. Nelson, B. Foster, and I. H. Gelman
Loss of the ssecks/gravin/akap12 Gene Results in Prostatic Hyperplasia
Cancer Res., July 1, 2008; 68(13): 5096 - 5103.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
Y. K. Choi, J. H. Kim, W. J. Kim, H. Y. Lee, J. A. Park, S.-W. Lee, D.-K. Yoon, H. H. Kim, H. Chung, Y. S. Yu, et al.
AKAP12 Regulates Human Blood-Retinal Barrier Formation by Downregulation of Hypoxia-Inducible Factor-1{alpha}
J. Neurosci., April 18, 2007; 27(16): 4472 - 4481.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Piontek and R. Brandt
Differential and Regulated Binding of cAMP-dependent Protein Kinase and Protein Kinase C Isoenzymes to Gravin in Human Model Neurons: EVIDENCE THAT GRAVIN PROVIDES A DYNAMIC PLATFORM FOR THE LOCALIZATION OF KINASES DURING NEURONAL DEVELOPMENT
J. Biol. Chem., October 3, 2003; 278(40): 38970 - 38979.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
W. K. Peitsch, I. Hofmann, N. Endlich, S. Pratzel, C. Kuhn, H. Spring, H.-J. Grone, W. Kriz, and W. W. Franke
Cell Biological and Biochemical Characterization of Drebrin Complexes in Mesangial Cells and Podocytes of Renal Glomeruli
J. Am. Soc. Nephrol., June 1, 2003; 14(6): 1452 - 1463.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
M. J. Wassler, C. I. Foote, I. H. Gelman, and B. D. Shur
Functional interaction between the SSeCKS scaffolding protein and the cytoplasmic domain of {beta}1,4-galactosyltransferase
J. Cell Sci., March 8, 2002; 114(12): 2291 - 2300.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
H. Kitamura, K. Okita, D. Fujikura, K. Mori, T. Iwanaga, and M. Saito
Induction of Src-suppressed C Kinase Substrate (SSeCKS) in Vascular Endothelial Cells by Bacterial Lipopolysaccharide
J. Histochem. Cytochem., February 1, 2002; 50(2): 245 - 256.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
X. Lin, P. Nelson, and I. H. Gelman
SSeCKS, a Major Protein Kinase C Substrate with Tumor Suppressor Activity, Regulates G1right-arrowS Progression by Controlling the Expression and Cellular Compartmentalization of Cyclin D
Mol. Cell. Biol., October 1, 2000; 20(19): 7259 - 7272.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 1999