|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 112, Issue 3 415-422, Copyright © 1999 by Company of Biologists
JOURNAL ARTICLES |
RJ Raggers, A van Helvoort, R Evers and G van Meer
Department of Cell Biology and Histology, Academic Medical Center, University of Amsterdam, PO Box 22700, The Netherlands.
Recently, we have provided evidence that the ABC-transporter MDR1 P-glycoprotein translocates analogs of various lipid classes across the apical plasma membrane of polarized LLC-PK1 cells transfected with MDR1 cDNA. Here, we show that expression of the basolateral ABC-transporter MRP1 (the multidrug resistance protein) induced lipid transport to the exoplasmic leaflet of the basolateral plasma membrane of LLC-PK1 cells at 15 degreesC. C6-NBD-glucosylceramide synthesized on the cytosolic side of the Golgi complex, but not C6-NBD-sphingomyelin synthesized in the Golgi lumen, became accessible to depletion by BSA in the basal culture medium. This suggests the absence of vesicular traffic and direct translocation of C6-NBD-glucosylceramide by MRP1 across the basolateral membrane. In line with this, transport of the lipid to the exoplasmic leaflet depended on the intracellular glutathione concentration and was inhibited by the MRP1-inhibitors sulfinpyrazone and indomethacin, but not by the MDR1 P-glycoprotein inhibitor PSC 833. In contrast to the broad substrate specificity of the MDR1 P-glycoprotein, MRP1 selectively transported C6-NBD-glucosylceramide and C6-NBD-sphingomyelin, the latter only when it was released from the Golgi lumen by brefeldin A. This shows the specific nature of the lipid translocation. We conclude that the transport activity of MDR1 P-glycoprotein and MRP1 must be taken into account in studies on the transport of lipids to the cell surface.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  R. van de Ven, R. Oerlemans, J. W. van der Heijden, G. L. Scheffer, T. D. de Gruijl, G. Jansen, and R. J. Scheper ABC drug transporters and immunity: novel therapeutic targets in autoimmunity and cancer J. Leukoc. Biol., November 1, 2009; 86(5): 1075 - 1087. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. L. Schnabl, M. Larcelet, A. B. R. Thomson, and M. T. Clandinin Uptake and fate of ganglioside GD3 in human intestinal Caco-2 cells Am J Physiol Gastrointest Liver Physiol, July 1, 2009; 297(1): G52 - G59. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. M. Argraves and W. S. Argraves HDL serves as a S1P signaling platform mediating a multitude of cardiovascular effects J. Lipid Res., November 1, 2007; 48(11): 2325 - 2333. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. L. Daleke Phospholipid Flippases J. Biol. Chem., January 12, 2007; 282(2): 821 - 825. [Full Text] [PDF]
|
|
|
|
 |
|
 P. Mitra, C. A. Oskeritzian, S. G. Payne, M. A. Beaven, S. Milstien, and S. Spiegel Role of ABCC1 in export of sphingosine-1-phosphate from mast cells PNAS, October 31, 2006; 103(44): 16394 - 16399. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A.-J. Dijkhuis, K. Klappe, W. Kamps, H. Sietsma, and J. W. Kok Gangliosides do not affect ABC transporter function in human neuroblastoma cells J. Lipid Res., June 1, 2006; 47(6): 1187 - 1195. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. E. Modrak, D. V. Gold, and D. M. Goldenberg Sphingolipid targets in cancer therapy. Mol. Cancer Ther., February 1, 2006; 5(2): 200 - 208. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Alder-Baerens, P. Muller, A. Pohl, T. Korte, Y. Hamon, G. Chimini, T. Pomorski, and A. Herrmann Headgroup-specific Exposure of Phospholipids in ABCA1-expressing Cells J. Biol. Chem., July 15, 2005; 280(28): 26321 - 26329. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. H. Malone, Z. Wang, and C. W. Distelhorst The Glucocorticoid-induced Gene tdag8 Encodes a Pro-apoptotic G Protein-coupled Receptor Whose Activation Promotes Glucocorticoid-induced Apoptosis J. Biol. Chem., December 17, 2004; 279(51): 52850 - 52859. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Pomorski, J. C. M. Holthuis, A. Herrmann, and G. van Meer Tracking down lipid flippases and their biological functions J. Cell Sci., February 22, 2004; 117(6): 805 - 813. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Reuter, T. Janvilisri, H. Venter, S. Shahi, L. Balakrishnan, and H. W. van Veen The ATP Binding Cassette Multidrug Transporter LmrA and Lipid Transporter MsbA Have Overlapping Substrate Specificities J. Biol. Chem., September 12, 2003; 278(37): 35193 - 35198. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Grzywacz, J.-M. Yang, and W. N. Hait Effect of the Multidrug Resistance Protein on the Transport of the Antiandrogen Flutamide Cancer Res., May 15, 2003; 63(10): 2492 - 2498. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. L. Daleke Regulation of transbilayer plasma membrane phospholipid asymmetry J. Lipid Res., February 1, 2003; 44(2): 233 - 242. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Maier and D. Hoekstra Trans-Golgi Network and Subapical Compartment of HepG2 Cells Display Different Properties in Sorting and Exiting of Sphingolipids J. Biol. Chem., January 3, 2003; 278(1): 164 - 173. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. H. Cha, T. Sekine, J.-i. Fukushima, Y. Kanai, Y. Kobayashi, T. Goya, and H. Endou Identification and Characterization of Human Organic Anion Transporter 3 Expressing Predominantly in the Kidney Mol. Pharmacol., April 16, 2001; 59(5): 1277 - 1286. [Abstract] [Full Text]
|
|
|
|
|
|
D. J. Sillence, R. J. Raggers, D. C. A. Neville, D. J. Harvey, and G. van Meer Assay for the transbilayer distribution of glycolipids: selective oxidation of glucosylceramide to glucuronylceramide by TEMPO nitroxyl radicals J. Lipid Res., August 1, 2000; 41(8): 1252 - 1260. [Abstract] [Full Text]
|
|
|
|
 |
|
 S. Hrafnsdóttir and A. K. Menon Reconstitution and Partial Characterization of Phospholipid Flippase Activity from Detergent Extracts of the Bacillus subtilis Cell Membrane J. Bacteriol., August 1, 2000; 182(15): 4198 - 4206. [Abstract] [Full Text]
|
|
|
|
|
|
P. Lala, S. Ito, and C. A. Lingwood Retroviral Transfection of Madin-Darby Canine Kidney Cells with Human MDR1 Results in a Major Increase in Globotriaosylceramide and 105- to 106-Fold Increased Cell Sensitivity to Verocytotoxin. ROLE OF P-GLYCOPROTEIN IN GLYCOLIPID SYNTHESIS J. Biol. Chem., February 25, 2000; 275(9): 6246 - 6251. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. T. Remaley, S. Rust, M. Rosier, C. Knapper, L. Naudin, C. Broccardo, K. M. Peterson, C. Koch, I. Arnould, C. Prades, et al. Human ATP-binding cassette transporter 1 (ABC1): Genomic organization and identification of the genetic defect in the original Tangier disease kindred PNAS, October 26, 1999; 96(22): 12685 - 12690. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Smith, A. van Helvoort, G. van Meer, K. Szabo, E. Welker, G. Szakacs, A. Varadi, B. Sarkadi, and P. Borst MDR3 P-glycoprotein, a Phosphatidylcholine Translocase, Transports Several Cytotoxic Drugs and Directly Interacts with Drugs as Judged by Interference with Nucleotide Trapping J. Biol. Chem., July 28, 2000; 275(31): 23530 - 23539. [Abstract] [Full Text] [PDF]
|
|
