|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 112, Issue 5 749-759, Copyright © 1999 by Company of Biologists
JOURNAL ARTICLES |
C Lang, M Paulin-Levasseur, A Gajewski, M Alsheimer, R Benavente and G Krohne
Division of Electron Microscopy, and Department of Cell and Developmental Biology, Biocenter of the University of Wurzburg, Am Hubland, D-97074 Wurzburg, Germany.
Lamina-associated polypeptides 2 (LAP2alpha, beta, gamma)/thymopoietins (TPalpha, beta, gamma) are a family of proteins that are generated by alternative splicing from a single gene. These proteins have been primarily characterized in mammals. One member of this protein family, the integral membrane protein LAP2beta/TPbeta, has been localized to the inner nuclear membrane of somatic cells where it binds to chromatin and B-type lamins. By cDNA cloning we have characterized XLAP2, a Xenopus homologue of the mammalian LAP2beta. Using LAP2-specific antibodies, the Mr 68,000 XLAP2 was found to be the only member of the LAP2/TP family expressed in somatic cells and adult tissues. XLAP2 was not detected in oocytes, eggs and in early embryos up to the gastrula stage at the mRNA and protein level demonstrating that it is not synthesized from maternal mRNA. In counterpart oocytes, eggs, and embryos contained one LAP2-related integral membrane proteins of Mr 84,000. Northern blot analysis with the XLAP2 cDNA showed that a single hybridizing mRNA band of 1.8-2.0 kb was present in Xenopus somatic cells whereas two other hybridizing mRNA species of 2.8-3.0 and 0. 9-1.1 kb were present in oocytes, eggs and early embryos. All together, these results indicated that at least three distinct LAP2-related proteins might be expressed in Xenopus. The LAP2/TP protein of Mr 84,000 is present in the early embryos but its amount decreases during embryogenesis concomitant with the increase of XLAP2 in the embryo. Our results are the first description of the developmentally regulated expression of integral nuclear envelope proteins during early embryogenesis.
This article has been cited by other articles:
|
|
 |
|
  G. Salpingidou, R. Rzepecki, E. Kiseleva, C. Lyon, B. Lane, K. Fusiek, A. Golebiewska, S. Drummond, T. Allen, J. A. Ellis, et al. NEP-A and NEP-B both contribute to nuclear pore formation in Xenopus eggs and oocytes J. Cell Sci., March 1, 2008; 121(5): 706 - 716. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. V. Broers, F. C. S. Ramaekers, G. Bonne, R. B. Yaou, and C. J. Hutchison Nuclear lamins: laminopathies and their role in premature ageing. Physiol Rev, July 1, 2006; 86(3): 967 - 1008. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. L. O'Brien and C. Wiese TPX2 is required for postmitotic nuclear assembly in cell-free Xenopus laevis egg extracts J. Cell Biol., June 5, 2006; 173(5): 685 - 694. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Lin, J. M. Morrison, W. Wu, and H. J. Worman MAN1, an integral protein of the inner nuclear membrane, binds Smad2 and Smad3 and antagonizes transforming growth factor-{beta} signaling Hum. Mol. Genet., February 1, 2005; 14(3): 437 - 445. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Wagner, D. Weber, S. Seitz, and G. Krohne The lamin B receptor of Drosophila melanogaster J. Cell Sci., April 15, 2004; 117(10): 2015 - 2028. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. K. Schoft, A. J. Beauvais, C. Lang, A. Gajewski, K. Prufert, C. Winkler, M.-A. Akimenko, M. Paulin-Levasseur, and G. Krohne The lamina-associated polypeptide 2 (LAP2) isoforms {beta}, {gamma} and {omega} of zebrafish: developmental expression and behavior during the cell cycle J. Cell Sci., June 15, 2003; 116(12): 2505 - 2517. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Vlcek, B. Korbei, and R. Foisner Distinct Functions of the Unique C Terminus of LAP2alpha in Cell Proliferation and Nuclear Assembly J. Biol. Chem., May 17, 2002; 277(21): 18898 - 18907. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Nili, G. S. Cojocaru, Y. Kalma, D. Ginsberg, N. G. Copeland, D. J. Gilbert, N. A. Jenkins, R. Berger, S. Shaklai, N. Amariglio, et al. Nuclear membrane protein LAP2{beta} mediates transcriptional repression alone and together with its binding partner GCL (germ-cell-less) J. Cell Sci., March 11, 2002; 114(18): 3297 - 3307. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Lin, D. L. Blake, I. Callebaut, I. S. Skerjanc, L. Holmer, M. W. McBurney, M. Paulin-Levasseur, and H. J. Worman MAN1, an Inner Nuclear Membrane Protein That Shares the LEM Domain with Lamina-associated Polypeptide 2 and Emerin J. Biol. Chem., February 18, 2000; 275(7): 4840 - 4847. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T Dechat, B Korbei, O. Vaughan, S Vlcek, C. Hutchison, and R Foisner Lamina-associated polypeptide 2alpha binds intranuclear A-type lamins J. Cell Sci., January 10, 2000; 113(19): 3473 - 3484. [Abstract] [PDF]
|
|
|
|
|
|
T. Sternsdorf, K. Jensen, B. Reich, and H. Will The Nuclear Dot Protein Sp100, Characterization of Domains Necessary for Dimerization, Subcellular Localization, and Modification by Small Ubiquitin-like Modifiers J. Biol. Chem., April 30, 1999; 274(18): 12555 - 12566. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Alsheimer, E. von Glasenapp, R. Hock, and R. Benavente Architecture of the Nuclear Periphery of Rat Pachytene Spermatocytes: Distribution of Nuclear Envelope Proteins in Relation to Synaptonemal Complex Attachment Sites Mol. Biol. Cell, April 1, 1999; 10(4): 1235 - 1245. [Abstract] [Full Text]
|
|
|
|
|
|
A Gajewski and G Krohne Subcellular distribution of the Xenopus p58/lamin B receptor in oocytes and eggs J. Cell Sci., January 8, 1999; 112(15): 2583 - 2596. [Abstract] [PDF]
|
|
