spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pountney, D.
Right arrow Articles by Beaumont, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pountney, D.
Right arrow Articles by Beaumont, C.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Journal of Cell Science, Vol 112, Issue 6 825-831, Copyright © 1999 by Company of Biologists

JOURNAL ARTICLES

The identification of ferritin in the nucleus of K562 cells, and investigation of a possible role in the transcriptional regulation of adult beta-globin gene expression

D Pountney, G Trugnan, M Bourgeois and C Beaumont
INSERM Unite 409, Faculte de Medecine Xavier Bichat, 75018 Paris, France.

We studied the subcellular distribution of ferritin in K562 cells by immunofluorescence techniques and have made a reappraisal of a direct binding interaction between ferritin and the proximal promoter region of the human beta-globin gene, as previously mentioned in the literature. Confocal microscopy indicates that ferritin, the iron-storage protein, is present in the nucleus of K562 cells, in addition to its expected cytoplasmic localisation. The stain distribution suggests that it is not directly associated with the nuclear matrix. Using a gel mobility shift assay, a protein that cross-reacts with monoclonal ferritin antibodies competitively binds to a double-stranded oligonucleotide spanning the region situated 150 base pairs upstream from the beta-globin transcription start site. Despite this antibody cross-reactivity, the protein is unlike cytosolic ferritin as it appears to be highly sensitive to both temperature and freeze-thaw cycles, and UV-crosslinking experiments indicate that the molecular mass of the protein factor lies between 90 and 100 kDa. In conclusion, while the intranuclear location of ferritin is described in the present study, ferritin is not in direct contact with the beta-globin promoter region.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 IOVSHome page
M. V. Nurminskaya, C. J. Talbot, D. I. Nurminsky, K. E. Beazley, and T. F. Linsenmayer
Nuclear Ferritin: A Ferritoid-Ferritin Complex in Corneal Epithelial Cells
Invest. Ophthalmol. Vis. Sci., August 1, 2009; 50(8): 3655 - 3661.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
K. Iwasaki, E. L. MacKenzie, K. Hailemariam, K. Sakamoto, and Y. Tsuji
Hemin-Mediated Regulation of an Antioxidant-Responsive Element of the Human Ferritin H Gene and Role of Ref-1 during Erythroid Differentiation of K562 Cells.
Mol. Cell. Biol., April 1, 2006; 26(7): 2845 - 2856.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. Surguladze, K. M. Thompson, J. L. Beard, J. R. Connor, and M. G. Fried
Interactions and Reactions of Ferritin with DNA
J. Biol. Chem., April 9, 2004; 279(15): 14694 - 14702.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. M. Millholland, J. M. Fitch, C. X. Cai, E. P. Gibney, K. E. Beazley, and T. F. Linsenmayer
Ferritoid, a Tissue-specific Nuclear Transport Protein for Ferritin in Corneal Epithelial Cells
J. Biol. Chem., June 20, 2003; 278(26): 23963 - 23970.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
K. J. Thompson, M. G. Fried, Z. Ye, P. Boyer, and J. R. Connor
Regulation, mechanisms and proposed function of ferritin translocation to cell nuclei
J. Cell Sci., May 15, 2002; 115(10): 2165 - 2177.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
R. H. Broyles, V. Belegu, C. R. DeWitt, S. N. Shah, C. A. Stewart, Q. N. Pye, and R. A. Floyd
Specific repression of beta -globin promoter activity by nuclear ferritin
PNAS, July 31, 2001; 98(16): 9145 - 9150.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 1999