|
|
|
|
|
||
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 112, Issue 8 1149-1158, Copyright © 1999 by Company of Biologists
JOURNAL ARTICLES |
K Hayashi, S Yonemura, T Matsui and S Tsukita
Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan.
Ezrin/radixin/moesin (ERM) proteins are thought to play an important role in organizing cortical actin-based cytoskeletons through cross-linkage of actin filaments with integral membrane proteins. Recent in vitro biochemical studies have revealed that ERM proteins phosphorylated on their COOH-terminal threonine residue (CPERMs) are active in their cross-linking activity, but this has not yet been evaluated in vivo. To immunofluorescently visualize CPERMs in cultured cells as well as tissues using a mAb specific for CPERMs, we developed a new fixation protocol using trichloroacetic acid (TCA) as a fixative. Immunoblotting analyses in combination with immunofluorescence microscopy showed that TCA effectively inactivated soluble phosphatases, which maintained the phosphorylation level of CPERMs during sample processing for immunofluorescence staining. Immunofluorescence microscopy with TCA-fixed samples revealed that CPERMs were exclusively associated with plasma membranes in a variety of cells and tissues, whereas total ERM proteins were distributed in both the cytoplasm and plasma membranes. Furthermore, the amounts of CPERMs were shown to be regulated in a cell and tissue type-dependent manner. These findings favored the notion that phosphorylation of the COOH-terminal threonine plays a key role in the regulation of the cross-linking activity of ERM proteins in vivo.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Favia, L. Guerra, T. Fanelli, R. A. Cardone, S. Monterisi, F. Di Sole, S. Castellani, M. Chen, U. Seidler, S. J. Reshkin, et al. Na+/H+ Exchanger Regulatory Factor 1 Overexpression-dependent Increase of Cytoskeleton Organization Is Fundamental in the Rescue of F508del Cystic Fibrosis Transmembrane Conductance Regulator in Human Airway CFBE41o- Cells Mol. Biol. Cell, January 1, 2010; 21(1): 73 - 86. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Nakano, S. Sekine, K. Ito, and T. Horie Correlation between Apical Localization of Abcc2/Mrp2 and Phosphorylation Status of Ezrin in Rat Intestine Drug Metab. Dispos., July 1, 2009; 37(7): 1521 - 1527. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Zhu, J. Hatakeyama, B. Zhang, J. Makdisi, C. Ender, and J. G. Forte Novel insights of the gastric gland organization revealed by chief cell specific expression of moesin Am J Physiol Gastrointest Liver Physiol, February 1, 2009; 296(2): G185 - G195. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Zhu, J. Hatakeyama, C. Chen, A. Shastri, K. Poon, and J. G. Forte Comparative study of ezrin phosphorylation among different tissues: more is good; too much is bad Am J Physiol Cell Physiol, July 1, 2008; 295(1): C192 - C202. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Zhao, E. Yaoita, M. Nameta, Y. Zhang, L. M. Cuellar, H. Fujinaka, B. Xu, Y. Yoshida, K. Hatakeyama, and T. Yamamoto Claudin-6 localized in tight junctions of rat podocytes Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2008; 294(6): R1856 - R1862. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. A. Wald, A. S. Oriolo, A. Mashukova, N. L. Fregien, A. H. Langshaw, and P. J. I. Salas Atypical protein kinase C (iota) activates ezrin in the apical domain of intestinal epithelial cells J. Cell Sci., March 1, 2008; 121(5): 644 - 654. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Furutani, H. Matsuno, M. Kawasaki, T. Sasaki, K. Mori, and Y. Yoshihara Interaction between Telencephalin and ERM Family Proteins Mediates Dendritic Filopodia Formation J. Neurosci., August 15, 2007; 27(33): 8866 - 8876. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Watanabe, H. Hosoya, and S. Yonemura Regulation of Myosin II Dynamics by Phosphorylation and Dephosphorylation of Its Light Chain in Epithelial Cells Mol. Biol. Cell, February 1, 2007; 18(2): 605 - 616. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. S. Kayser, A. C. McClelland, E. G. Hughes, and M. B. Dalva Intracellular and Trans-Synaptic Regulation of Glutamatergic Synaptogenesis by EphB Receptors. J. Neurosci., November 22, 2006; 26(47): 12152 - 12164. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. C. Hughes and R. G. Fehon Phosphorylation and activity of the tumor suppressor Merlin and the ERM protein Moesin are coordinately regulated by the Slik kinase J. Cell Biol., October 23, 2006; 175(2): 305 - 313. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Otani, T. Ichii, S. Aono, and M. Takeichi Cdc42 GEF Tuba regulates the junctional configuration of simple epithelial cells J. Cell Biol., October 9, 2006; 175(1): 135 - 146. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M. Grimsley, M. Lu, L. B. Haney, J. M. Kinchen, and K. S. Ravichandran Characterization of a Novel Interaction between ELMO1 and ERM Proteins J. Biol. Chem., March 3, 2006; 281(9): 5928 - 5937. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Koss, G. R. Pfeiffer II, Y. Wang, S. T. Thomas, M. Yerukhimovich, W. A. Gaarde, C. M. Doerschuk, and Q. Wang Ezrin/Radixin/Moesin Proteins Are Phosphorylated by TNF-{alpha} and Modulate Permeability Increases in Human Pulmonary Microvascular Endothelial Cells J. Immunol., January 15, 2006; 176(2): 1218 - 1227. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Maeda, Y. Shintani, M. J. Wheelock, and K. R. Johnson Src Activation Is Not Necessary for Transforming Growth Factor (TGF)-{beta}-mediated Epithelial to Mesenchymal Transitions (EMT) in Mammary Epithelial Cells: PP1 DIRECTLY INHIBITS TGF-{beta} RECEPTORS I AND II J. Biol. Chem., January 6, 2006; 281(1): 59 - 68. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Nishimura and S. Yonemura Centralspindlin regulates ECT2 and RhoA accumulation at the equatorial cortex during cytokinesis J. Cell Sci., January 1, 2006; 119(1): 104 - 114. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Zhou, L. Zhu, A. Kodani, P. Hauser, X. Yao, and J. G. Forte Phosphorylation of ezrin on threonine 567 produces a change in secretory phenotype and repolarizes the gastric parietal cell J. Cell Sci., October 1, 2005; 118(19): 4381 - 4391. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W.-m. Zhao and G. Fang Anillin Is a Substrate of Anaphase-promoting Complex/Cyclosome (APC/C) That Controls Spatial Contractility of Myosin during Late Cytokinesis J. Biol. Chem., September 30, 2005; 280(39): 33516 - 33524. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W.-m. Zhao and G. Fang MgcRacGAP controls the assembly of the contractile ring and the initiation of cytokinesis PNAS, September 13, 2005; 102(37): 13158 - 13163. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Tamma, E. Klussmann, J. Oehlke, E. Krause, W. Rosenthal, M. Svelto, and G. Valenti Actin remodeling requires ERM function to facilitate AQP2 apical targeting J. Cell Sci., August 15, 2005; 118(16): 3623 - 3630. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. H. Cant and J. A. Pitcher G Protein-coupled Receptor Kinase 2-mediated Phosphorylation of Ezrin Is Required for G Protein-coupled Receptor-dependent Reorganization of the Actin Cytoskeleton Mol. Biol. Cell, July 1, 2005; 16(7): 3088 - 3099. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Yokoyama, H. Goto, I. Izawa, H. Mizutani, and M. Inagaki Aurora-B and Rho-kinase/ROCK, the two cleavage furrow kinases, independently regulate the progression of cytokinesis: possible existence of a novel cleavage furrow kinase phosphorylates ezrin/radixin/moesin (ERM) Genes Cells, February 1, 2005; 10(2): 127 - 137. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. C. Morales, Y. Takahashi, E. L. Kreimann, and M.-M. Georgescu Ezrin-radixin-moesin (ERM)-binding phosphoprotein 50 organizes ERM proteins at the apical membrane of polarized epithelia PNAS, December 21, 2004; 101(51): 17705 - 17710. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Hatakeyama, Y. Bessho, K. Katoh, S. Ookawara, M. Fujioka, F. Guillemot, and R. Kageyama Hes genes regulate size, shape and histogenesis of the nervous system by control of the timing of neural stem cell differentiation Development, November 15, 2004; 131(22): 5539 - 5550. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Wada, A. Ogawa, Y. Hanai, A. Nakamura, M. Maki, and K. Hitomi Analyses of Expression and Localization of Two Mammalian-Type Transglutaminases in Physarum polycephalum, an Acellular Slime Mold J. Biochem., November 1, 2004; 136(5): 665 - 672. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-H. Lee, T. Katakai, T. Hara, H. Gonda, M. Sugai, and A. Shimizu Roles of p-ERM and Rho-ROCK signaling in lymphocyte polarity and uropod formation J. Cell Biol., October 25, 2004; 167(2): 327 - 337. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Umeda, T. Matsui, M. Nakayama, K. Furuse, H. Sasaki, M. Furuse, and S. Tsukita Establishment and Characterization of Cultured Epithelial Cells Lacking Expression of ZO-1 J. Biol. Chem., October 22, 2004; 279(43): 44785 - 44794. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-i. Kitajiri, T. Miyamoto, A. Mineharu, N. Sonoda, K. Furuse, M. Hata, H. Sasaki, Y. Mori, T. Kubota, J. Ito, et al. Compartmentalization established by claudin-11-based tight junctions in stria vascularis is required for hearing through generation of endocochlear potential J. Cell Sci., October 1, 2004; 117(21): 5087 - 5096. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-i. Kitajiri, K. Fukumoto, M. Hata, H. Sasaki, T. Katsuno, T. Nakagawa, J. Ito, S. Tsukita, and S. Tsukita Radixin deficiency causes deafness associated with progressive degeneration of cochlear stereocilia J. Cell Biol., August 16, 2004; 166(4): 559 - 570. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Finnerty, D. Chambers, J. Ingraffea, H. R. Faber, P. A. Karplus, and A. Bretscher The EBP50-moesin interaction involves a binding site regulated by direct masking on the FERM domain J. Cell Sci., March 15, 2004; 117(8): 1547 - 1552. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Matsuda, A. Kubo, M. Furuse, and S. Tsukita A peculiar internalization of claudins, tight junction-specific adhesion molecules, during the intercellular movement of epithelial cells J. Cell Sci., March 1, 2004; 117(7): 1247 - 1257. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. T. Fievet, A. Gautreau, C. Roy, L. Del Maestro, P. Mangeat, D. Louvard, and M. Arpin Phosphoinositide binding and phosphorylation act sequentially in the activation mechanism of ezrin J. Cell Biol., March 1, 2004; 164(5): 653 - 659. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Huang, Y. You, M. S. Spoor, E. J. Richer, Vrinda. V. Kudva, R. C. Paige, M. P. Seiler, J. M. Liebler, J. Zabner, C. G. Plopper, et al. Foxj1 is required for apical localization of ezrin in airway epithelial cells J. Cell Sci., December 15, 2003; 116(24): 4935 - 4945. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Brown, R. Nijhara, J. A. Hallam, M. Gignac, K. M. Yamada, S. L. Erlandsen, J. Delon, M. Kruhlak, and S. Shaw Chemokine stimulation of human peripheral blood T lymphocytes induces rapid dephosphorylation of ERM proteins, which facilitates loss of microvilli and polarization Blood, December 1, 2003; 102(12): 3890 - 3899. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Nishizuka, S.-T. Chen, F. G. Gwadry, J. Alexander, S. M. Major, U. Scherf, W. C. Reinhold, M. Waltham, L. Charboneau, L. Young, et al. Diagnostic Markers That Distinguish Colon and Ovarian Adenocarcinomas: Identification by Genomic, Proteomic, and Tissue Array Profiling Cancer Res., September 1, 2003; 63(17): 5243 - 5250. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Leach, S. Shenolikar, and D. L. Brautigan Phosphorylation of Phosphatase Inhibitor-2 at Centrosomes during Mitosis J. Biol. Chem., July 3, 2003; 278(28): 26015 - 26020. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. W. Thompson, A. M. Randi, and A. J. Ridley Intercellular Adhesion Molecule (ICAM)-1, But Not ICAM-2, Activates RhoA and Stimulates c-fos and rhoA Transcription in Endothelial Cells J. Immunol., July 15, 2002; 169(2): 1007 - 1013. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Barreiro, M. Yanez-Mo, J. M. Serrador, M. C. Montoya, M. Vicente-Manzanares, R. Tejedor, H. Furthmayr, and F. Sanchez-Madrid Dynamic interaction of VCAM-1 and ICAM-1 with moesin and ezrin in a novel endothelial docking structure for adherent leukocytes J. Cell Biol., June 24, 2002; 157(7): 1233 - 1245. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Yonemura, T. Matsui, S. Tsukita, and S. Tsukita Rho-dependent and -independent activation mechanisms of ezrin/radixin/moesin proteins: an essential role for polyphosphoinositides in vivo J. Cell Sci., June 15, 2002; 115(12): 2569 - 2580. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Kiuchi-Saishin, S. Gotoh, M. Furuse, A. Takasuga, Y. Tano, and S. Tsukita Differential Expression Patterns of Claudins, Tight Junction Membrane Proteins, in Mouse Nephron Segments J. Am. Soc. Nephrol., April 1, 2002; 13(4): 875 - 886. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Serrador, M. Vicente-Manzanares, J. Calvo, O. Barreiro, M. C. Montoya, R. Schwartz-Albiez, H. Furthmayr, F. Lozano, and F. Sanchez-Madrid A Novel Serine-rich Motif in the Intercellular Adhesion Molecule 3 Is Critical for Its Ezrin/Radixin/Moesin-directed Subcellular Targeting J. Biol. Chem., March 15, 2002; 277(12): 10400 - 10409. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Eda, S. Yonemura, T. Kato, N. Watanabe, T. Ishizaki, P. Madaule, and S. Narumiya Rho-dependent transfer of Citron-kinase to the cleavage furrow of dividing cells J. Cell Sci., March 11, 2002; 114(18): 3273 - 3284. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Cukierman, R. Pankov, D. R. Stevens, and K. M. Yamada Taking Cell-Matrix Adhesions to the Third Dimension Science, November 23, 2001; 294(5547): 1708 - 1712. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Simonovic, M. Arpin, A. Koutsouris, H. J. Falk-Krzesinski, and G. Hecht Enteropathogenic Escherichia coli Activates Ezrin, Which Participates in Disruption of Tight Junction Barrier Function Infect. Immun., September 1, 2001; 69(9): 5679 - 5688. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Furuse, K. Furuse, H. Sasaki, and S. Tsukita Conversion of Zonulae Occludentes from Tight to Leaky Strand Type by Introducing Claudin-2 into Madin-Darby Canine Kidney I Cells J. Cell Biol., April 16, 2001; 153(2): 263 - 272. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. V. Melendez-Vasquez, J. C. Rios, G. Zanazzi, S. Lambert, A. Bretscher, and J. L. Salzer Nodes of Ranvier form in association with ezrin-radixin-moesin (ERM)-positive Schwann cell processes PNAS, January 30, 2001; 98(3): 1235 - 1240. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Gautreau, D. Louvard, and M. Arpin Morphogenic Effects of Ezrin Require a Phosphorylation-Induced Transition from Oligomers to Monomers at the Plasma Membrane J. Cell Biol., July 10, 2000; 150(1): 193 - 204. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Tsukita and S. Yonemura Cortical Actin Organization: Lessons from ERM (Ezrin/Radixin/Moesin) Proteins J. Biol. Chem., December 3, 1999; 274(49): 34507 - 34510. [Full Text] [PDF]
|
|
|
|
|
|
N. Sonoda, M. Furuse, H. Sasaki, S. Yonemura, J. Katahira, Y. Horiguchi, and S. Tsukita Clostridium perfringens Enterotoxin Fragment Removes Specific Claudins from Tight Junction Strands: Evidence for Direct Involvement of Claudins in Tight Junction Barrier J. Cell Biol., October 4, 1999; 147(1): 195 - 204. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Yonemura, S. Tsukita, and S. Tsukita Direct Involvement of Ezrin/Radixin/Moesin (ERM)-binding Membrane Proteins in the Organization of Microvilli in Collaboration with Activated ERM Proteins J. Cell Biol., June 28, 1999; 145(7): 1497 - 1509. [Abstract] [Full Text] [PDF]
|
|
