Journal of Cell Science, Vol 112, Issue 8 1257-1271, Copyright © 1999 by Company of Biologists

JOURNAL ARTICLES

The growth-related, translationally controlled protein P23 has properties of a tubulin binding protein and associates transiently with microtubules during the cell cycle

Y Gachet, S Tournier, M Lee, A Lazaris-Karatzas, T Poulton and UA Bommer
Divisions of Biochemistry and Immunology, St George's Hospital Medical School, London, UK.

The translationally controlled protein P23 was discovered by the early induction of its rate of synthesis after mitogenic stimulation of mouse fibroblasts. P23 is expressed in almost all mammalian tissues and it is highly conserved between animals, plants and yeast. Based on its amino acid sequence, P23 cannot be attributed to any known protein family, and its cellular function remains to be elucidated. Here, we present evidence that P23 has properties of a tubulin binding protein that associates with microtubules in a cell cycle-dependent manner. (1) P23 is a cytoplasmic protein that occurs in complexes of 100-150 kDa, and part of P23 can be immunoprecipitated from HeLa cell extracts with anti-tubulin antibodies. (2) In immunolocalisation experiments we find P23 associated with microtubules during G1, S, G2 and early M phase of the cell cycle. At metaphase, P23 is also bound to the mitotic spindle, and it is detached from the spindle during metaphase-anaphase transition. (3) A GST-P23 fusion protein interacts with alpha- and beta-tubulin, and recombinant P23 binds to taxol-stabilised microtubules in vitro. The tubulin binding domain of P23 was identified by mutational analysis; it shows similarity to part of the tubulin binding domain of the microtubule-associated protein MAP-1B. (4) Overexpression of P23 results in cell growth retardation and in alterations of cell morphology. Moreover, elevation of P23 levels leads to microtubule rearrangements and to an increase in microtubule mass and stability.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				F. Bazile, A. Pascal, I. Arnal, C. Le Clainche, F. Chesnel, and J. Z. Kubiak Complex relationship between TCTP, microtubules and actin microfilaments regulates cell shape in normal and cancer cells Carcinogenesis, April 1, 2009; 30(4): 555 - 565. [Abstract] [Full Text] [PDF]

				O. Berkowitz, R. Jost, S. Pollmann, and J. Masle Characterization of TCTP, the Translationally Controlled Tumor Protein, from Arabidopsis thaliana PLANT CELL, December 1, 2008; 20(12): 3430 - 3447. [Abstract] [Full Text] [PDF]

				X. Wang, B. D. Fonseca, H. Tang, R. Liu, A. Elia, M. J. Clemens, U.-A. Bommer, and C. G. Proud Re-evaluating the Roles of Proposed Modulators of Mammalian Target of Rapamycin Complex 1 (mTORC1) Signaling J. Biol. Chem., November 7, 2008; 283(45): 30482 - 30492. [Abstract] [Full Text] [PDF]

				G. Barel and I. Ginzberg Potato skin proteome is enriched with plant defence components J. Exp. Bot., September 1, 2008; 59(12): 3347 - 3357. [Abstract] [Full Text] [PDF]

				S. H. Chen, P.-S. Wu, C.-H. Chou, Y.-T. Yan, H. Liu, S.-Y. Weng, and H.-F. Yang-Yen A Knockout Mouse Approach Reveals that TCTP Functions as an Essential Factor for Cell Proliferation and Survival in a Tissue- or Cell Type-specific Manner Mol. Biol. Cell, July 1, 2007; 18(7): 2525 - 2532. [Abstract] [Full Text] [PDF]

				C Doering-Saad, H. Newbury, C. Couldridge, J. Bale, and J Pritchard A phloem-enriched cDNA library from Ricinus: insights into phloem function J. Exp. Bot., September 1, 2006; 57(12): 3183 - 3193. [Abstract] [Full Text] [PDF]

				D. M. Smith-Beckerman, K. W. Fung, K. E. Williams, N. Auersperg, A. K. Godwin, and A. L. Burlingame Proteome Changes in Ovarian Epithelial Cells Derived from Women with BRCA1 Mutations and Family Histories of Cancer Mol. Cell. Proteomics, February 1, 2005; 4(2): 156 - 168. [Abstract] [Full Text] [PDF]

				J. Jung, M. Kim, M.-J. Kim, J. Kim, J. Moon, J.-S. Lim, M. Kim, and K. Lee Translationally Controlled Tumor Protein Interacts with the Third Cytoplasmic Domain of Na,K-ATPase {alpha} Subunit and Inhibits the Pump Activity in HeLa Cells J. Biol. Chem., November 26, 2004; 279(48): 49868 - 49875. [Abstract] [Full Text] [PDF]

				N. Amzallag, B. J. Passer, D. Allanic, E. Segura, C. Thery, B. Goud, R. Amson, and A. Telerman TSAP6 Facilitates the Secretion of Translationally Controlled Tumor Protein/Histamine-releasing Factor via a Nonclassical Pathway J. Biol. Chem., October 29, 2004; 279(44): 46104 - 46112. [Abstract] [Full Text] [PDF]

				M. Tuynder, G. Fiucci, S. Prieur, A. Lespagnol, A. Geant, S. Beaucourt, D. Duflaut, S. Besse, L. Susini, J. Cavarelli, et al. Translationally controlled tumor protein is a target of tumor reversion PNAS, October 26, 2004; 101(43): 15364 - 15369. [Abstract] [Full Text] [PDF]

				D. R. Rundle, G. Gorbsky, and L. Tsiokas PKD2 Interacts and Co-localizes with mDia1 to Mitotic Spindles of Dividing Cells: ROLE OF mDia1 IN PKD2 LOCALIZATION TO MITOTIC SPINDLES J. Biol. Chem., July 9, 2004; 279(28): 29728 - 29739. [Abstract] [Full Text] [PDF]

				K. Yoneda, K. Rokutan, Y. Nakamura, H. Yanagawa, S. Kondo-Teshima, and S. Sone Stimulation of human bronchial epithelial cells by IgE-dependent histamine-releasing factor Am J Physiol Lung Cell Mol Physiol, January 1, 2004; 286(1): L174 - L181. [Abstract] [Full Text] [PDF]

				C. Cans, B. J. Passer, V. Shalak, V. Nancy-Portebois, V. Crible, N. Amzallag, D. Allanic, R. Tufino, M. Argentini, D. Moras, et al. Translationally controlled tumor protein acts as a guanine nucleotide dissociation inhibitor on the translation elongation factor eEF1A PNAS, November 25, 2003; 100(24): 13892 - 13897. [Abstract] [Full Text] [PDF]

				N. M. Verrills, B. J. Walsh, G. S. Cobon, P. G. Hains, and M. Kavallaris Proteome Analysis of Vinca Alkaloid Response and Resistance in Acute Lymphoblastic Leukemia Reveals Novel Cytoskeletal Alterations J. Biol. Chem., November 14, 2003; 278(46): 45082 - 45093. [Abstract] [Full Text] [PDF]

				D. A. Tulis, Z. H. Mnjoyan, R. L. Schiesser, H. S. Shelat, A. J. Evans, P. Zoldhelyi, and K. Fujise Adenoviral Gene Transfer of Fortilin Attenuates Neointima Formation Through Suppression of Vascular Smooth Muscle Cell Proliferation and Migration Circulation, January 7, 2003; 107(1): 98 - 105. [Abstract] [Full Text] [PDF]

				F. R. Yarm Plk Phosphorylation Regulates the Microtubule-Stabilizing Protein TCTP Mol. Cell. Biol., September 1, 2002; 22(17): 6209 - 6221. [Abstract] [Full Text] [PDF]

				K. V. N. Rao, L. Chen, M. Gnanasekar, and K. Ramaswamy Cloning and Characterization of a Calcium-binding, Histamine-releasing Protein from Schistosoma mansoni J. Biol. Chem., August 16, 2002; 277(34): 31207 - 31213. [Abstract] [Full Text] [PDF]

				M. Bifulco, C. Laezza, S. Stingo, and J. Wolff 2',3'-Cyclic nucleotide 3'-phosphodiesterase: A membrane-bound, microtubule-associated protein and membrane anchor for tubulin PNAS, February 19, 2002; 99(4): 1807 - 1812. [Abstract] [Full Text] [PDF]