spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by James, S. W.
Right arrow Articles by Wade, S. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by James, S. W.
Right arrow Articles by Wade, S. D.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Journal of Cell Science, Vol 112, Issue 9 1313-1324, Copyright © 1999 by Company of Biologists

JOURNAL ARTICLES

nimO, an Aspergillus gene related to budding yeast Dbf4, is required for DNA synthesis and mitotic checkpoint control

SW James, KA Bullock, SE Gygax, BA Kraynack, RA Matura, JA MacLeod, KK McNeal, KA Prasauckas, PC Scacheri, HL Shenefiel, HM Tobin and SD Wade
Department of Biology, Gettysburg College, Gettysburg, PA 17325, USA. sjames@gettysburg.edu

The nimO predicted protein of Aspergillus nidulans is related structurally and functionally to Dbf4p, the regulatory subunit of Cdc7p kinase in budding yeast. nimOp and Dbf4p are most similar in their C-termini, which contain a PEST motif and a novel, short-looped Cys2-His2 zinc finger-like motif. DNA labelling and reciprocal shift assays using ts-lethal nimO18 mutants showed that nimO is required for initiation of DNA synthesis and for efficient progression through S phase. nimO18 mutants abrogated a cell cycle checkpoint linking S and M phases by segregating their unreplicated chromatin. This checkpoint defect did not interfere with other checkpoints monitoring spindle assembly and DNA damage (dimer lesions), but did prevent activation of a DNA replication checkpoint. The division of unreplicated chromatin was accelerated in cells lacking a component of the anaphase-promoting complex (bimEAPC1), consistent with the involvement of nimO and APC/C in separate checkpoint pathways. A nimO deletion conferred DNA synthesis and checkpoint defects similar to nimO18. Inducible nimO alleles lacking as many as 244 C-terminal amino acids supported hyphal growth, but not asexual development, when overexpressed in a ts-lethal nimO18 strain. However, the truncated alleles could not rescue a nimO deletion, indicating that the C terminus is essential and suggesting some type of interaction among nimO polypeptides.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
A. D. Fung, J. Ou, S. Bueler, and G. W. Brown
A Conserved Domain of Schizosaccharomyces pombe dfp1+ Is Uniquely Required for Chromosome Stability following Alkylation Damage during S Phase
Mol. Cell. Biol., July 1, 2002; 22(13): 4477 - 4490.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
R. Nougarède, F. Della Seta, P. Zarzov, and E. Schwob
Hierarchy of S-Phase-Promoting Factors: Yeast Dbf4-Cdc7 Kinase Requires Prior S-Phase Cyclin-Dependent Kinase Activation
Mol. Cell. Biol., June 1, 2000; 20(11): 3795 - 3806.
[Abstract] [Full Text]

Home page
 J. Cell Sci.Home page
R. Sclafani
Cdc7p-Dbf4p becomes famous in the cell cycle
J. Cell Sci., January 6, 2000; 113(12): 2111 - 2117.
[Abstract] [PDF]

Home page
 J. Biol. Chem.Home page
K. Ogino, T. Takeda, E. Matsui, H. Iiyama, C. Taniyama, K.-i. Arai, and H. Masai
Bipartite Binding of a Kinase Activator Activates Cdc7-related Kinase Essential for S Phase
J. Biol. Chem., August 10, 2001; 276(33): 31376 - 31387.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
K. E. Sharpless and S. D. Harris
Functional Characterization and Localization of the Aspergillus nidulans Formin SEPA
Mol. Biol. Cell, February 1, 2002; 13(2): 469 - 479.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 1999