|
|
|
|
|
||
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 113, Issue 1 135-144, Copyright © 2000 by Company of Biologists
JOURNAL ARTICLES |
R Pepperkok, JA Whitney, M Gomez and TE Kreis
Cell Biophysics and Cell Biology Program, EMBL Heidelberg, Meyerhofstr.1, Germany. pepperko@embl-heidelberg.de.
Microinjection of the slowly hydrolyzable GTP analogue GTP(gamma)S or the ectopic expression of a GTP restricted mutant of the small GTPase arf1 (arf1[Q71L]) leads to the rapid accumulation of COPI coated vesicles and buds in living cells. This effect is blocked at 15 degrees C and by microinjection of antibodies against (beta)-COP. Anterograde and retrograde membrane protein transport markers, which have been previously shown to be incorporated into COPI vesicles between the endoplasmic reticulum and Golgi complex, are depleted from the GTP(gamma)S or arf1[Q71L] induced COPI coated vesicles and buds. In contrast, in control cells 30 to 60% of the COPI carriers co-localize with these markers. These in vivo data corroborate recent in vitro work, suggesting that GTP(gamma)S and arf1[Q71L] interfere with the sorting of membrane proteins into Golgi derived COPI vesicles, and provide the first in vivo evidence for a role of GTP hydrolysis by arf1 in the sorting of cargo into COPI coated vesicles and buds.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  A. Saitoh, H.-W. Shin, A. Yamada, S. Waguri, and K. Nakayama Three Homologous ArfGAPs Participate in Coat Protein I-mediated Transport J. Biol. Chem., May 15, 2009; 284(20): 13948 - 13957. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Weimer, R. Beck, P. Eckert, I. Reckmann, J. Moelleken, B. Brugger, and F. Wieland Differential roles of ArfGAP1, ArfGAP2, and ArfGAP3 in COPI trafficking J. Cell Biol., November 18, 2008; 183(4): 725 - 735. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Foret and P. Sens Kinetic regulation of coated vesicle secretion PNAS, September 30, 2008; 105(39): 14763 - 14768. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Rennolds, C. Tower, L. Musgrove, L. Fan, K. Maloney, J. P. Clancy, K. L. Kirk, E. Sztul, and E. Cormet-Boyaka Cystic Fibrosis Transmembrane Conductance Regulator Trafficking Is Mediated by the COPI Coat in Epithelial Cells J. Biol. Chem., January 11, 2008; 283(2): 833 - 839. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Moelleken, J. Malsam, M. J. Betts, A. Movafeghi, I. Reckmann, I. Meissner, A. Hellwig, R. B. Russell, T. Sollner, B. Brugger, et al. Differential localization of coatomer complex isoforms within the Golgi apparatus PNAS, March 13, 2007; 104(11): 4425 - 4430. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Bethune, M. Kol, J. Hoffmann, I. Reckmann, B. Brugger, and F. Wieland Coatomer, the Coat Protein of COPI Transport Vesicles, Discriminates Endoplasmic Reticulum Residents from p24 Proteins Mol. Cell. Biol., November 1, 2006; 26(21): 8011 - 8021. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Yahara, K. Sato, and A. Nakano The Arf1p GTPase-activating protein Glo3p executes its regulatory function through a conserved repeat motif at its C-terminus J. Cell Sci., June 15, 2006; 119(12): 2604 - 2612. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Frankland, A. Adisa, P. Horrocks, T. F. Taraschi, T. Schneider, S. R. Elliott, S. J. Rogerson, E. Knuepfer, A. F. Cowman, C. I. Newbold, et al. Delivery of the Malaria Virulence Protein PfEMP1 to the Erythrocyte Surface Requires Cholesterol-Rich Domains Eukaryot. Cell, May 1, 2006; 5(5): 849 - 860. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Nie and P. A. Randazzo Arf GAPs and membrane traffic. J. Cell Sci., April 1, 2006; 119(Pt 7): 1203 - 1211. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Robinson, P. P. Poon, C. Schindler, L. E. Murray, R. Kama, G. Gabriely, R. A. Singer, A. Spang, G. C. Johnston, and J. E. Gerst The Gcs1 Arf-GAP Mediates Snc1,2 v-SNARE Retrieval to the Golgi in Yeast Mol. Biol. Cell, April 1, 2006; 17(4): 1845 - 1858. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. W. McCartney, J. S. Greenwood, M. R. Fabian, K. A. White, and R. T. Mullen Localization of the Tomato Bushy Stunt Virus Replication Protein p33 Reveals a Peroxisome-to-Endoplasmic Reticulum Sorting Pathway PLANT CELL, December 1, 2005; 17(12): 3513 - 3531. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Meyer, P. Crottet, B. Maco, E. Degtyar, D. Cassel, and M. Spiess Oligomerization and Dissociation of AP-1 Adaptors Are Regulated by Cargo Signals and by ArfGAP1-induced GTP Hydrolysis Mol. Biol. Cell, October 1, 2005; 16(10): 4745 - 4754. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Xu and B. Scheres Dissection of Arabidopsis ADP-RIBOSYLATION FACTOR 1 Function in Epidermal Cell Polarity PLANT CELL, February 1, 2005; 17(2): 525 - 536. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Y. Lee, J.-S. Yang, W. Hong, R. T. Premont, and V. W. Hsu ARFGAP1 plays a central role in coupling COPI cargo sorting with vesicle formation J. Cell Biol., January 17, 2005; 168(2): 281 - 290. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. D. Song, M. Leonard, and S. L. Schmid Dynamin GTPase Domain Mutants That Differentially Affect GTP Binding, GTP Hydrolysis, and Clathrin-mediated Endocytosis J. Biol. Chem., September 24, 2004; 279(39): 40431 - 40436. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Lewis, P. P. Poon, R. A. Singer, G. C. Johnston, and A. Spang The ArfGAP Glo3 Is Required for the Generation of COPI Vesicles Mol. Biol. Cell, September 1, 2004; 15(9): 4064 - 4072. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Stephens and R. Pepperkok Differential effects of a GTP-restricted mutant of Sar1p on segregation of cargo during export from the endoplasmic reticulum J. Cell Sci., July 15, 2004; 117(16): 3635 - 3644. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Liu, J. J. Flanagan, and C. Barlowe Sec22p Export from the Endoplasmic Reticulum Is Independent of SNARE Pairing J. Biol. Chem., June 25, 2004; 279(26): 27225 - 27232. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Reinhard, M. Schweikert, F. T. Wieland, and W. Nickel Functional reconstitution of COPI coat assembly and disassembly using chemically defined components PNAS, July 8, 2003; 100(14): 8253 - 8257. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Pimpl, S. L. Hanton, J. P. Taylor, L. L. Pinto-daSilva, and J. Denecke The GTPase ARF1p Controls the Sequence-Specific Vacuolar Sorting Route to the Lytic Vacuole PLANT CELL, May 1, 2003; 15(5): 1242 - 1256. [Abstract] [Full Text]
|
|
|
|
|
|
Y. Liu and C. Barlowe Analysis of Sec22p in Endoplasmic Reticulum/Golgi Transport Reveals Cellular Redundancy in SNARE Protein Function Mol. Biol. Cell, September 1, 2002; 13(9): 3314 - 3324. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Nickel, B. Brugger, and F. T. Wieland Vesicular transport: the core machinery of COPI recruitment and budding J. Cell Sci., August 15, 2002; 115(16): 3235 - 3240. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Stephens and R. Pepperkok Imaging of procollagen transport reveals COPI-dependent cargo sorting during ER-to-Golgi transport in mammalian cells J. Cell Sci., March 15, 2002; 115(6): 1149 - 1160. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M.K. Tsui, W. C.S. Tai, and D. K. Banfield Selective Formation of Sed5p-containing SNARE Complexes Is Mediated by Combinatorial Binding Interactions Mol. Biol. Cell, March 1, 2001; 12(3): 521 - 538. [Abstract] [Full Text]
|
|
|
|
|
|
P. Pimpl, A. Movafeghi, S. Coughlan, J. Denecke, S. Hillmer, and D. G. Robinson In Situ Localization and in Vitro Induction of Plant COPI-Coated Vesicles PLANT CELL, November 1, 2000; 12(11): 2219 - 2236. [Abstract] [Full Text]
|
|
|
|
|
|
B. Brugger, R. Sandhoff, S. Wegehingel, K. Gorgas, J. Malsam, J. B. Helms, W.-D. Lehmann, W. Nickel, and F. T. Wieland Evidence for Segregation of Sphingomyelin and Cholesterol during Formation of Copi-Coated Vesicles J. Cell Biol., October 30, 2000; 151(3): 507 - 518. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M Ying, T Flatmark, and J Saraste The p58-positive pre-golgi intermediates consist of distinct subpopulations of particles that show differential binding of COPI and COPII coats and contain vacuolar H(+)-ATPase J. Cell Sci., January 10, 2000; 113(20): 3623 - 3638. [Abstract] [PDF]
|
|
|
|
|
|
A. Charest, K. Lane, K. McMahon, and D. E. Housman Association of a Novel PDZ Domain-containing Peripheral Golgi Protein with the Q-SNARE (Q-soluble N-Ethylmaleimide-sensitive Fusion Protein (NSF) Attachment Protein Receptor) Protein Syntaxin 6 J. Biol. Chem., July 27, 2001; 276(31): 29456 - 29465. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Martinez-Menarguez, R. Prekeris, V. M.J. Oorschot, R. Scheller, J. W. Slot, H. J. Geuze, and J. Klumperman Peri-Golgi vesicles contain retrograde but not anterograde proteins consistent with the cisternal progression model of intra-Golgi transport J. Cell Biol., December 24, 2001; 155(7): 1213 - 1224. [Abstract] [Full Text] [PDF]
|
|
