|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 113, Issue 10 1747-1758, Copyright © 2000 by Company of Biologists
JOURNAL ARTICLES |
R Graf, C Daunderer and M Schliwa
Adolf-Butenandt-Institut, Zellbiologie, Schillerstr. 42, D-80336 Munchen, Germany. ralph.graef@lrz.uni-muenchen.de
A cDNA encoding a 224-kDa Dictyostelium discoideum centrosomal protein (DdCP224) was isolated by immunoscreening. DdCP224 was detected at the centrosome and, more weakly, along microtubules throughout the entire cell cycle. Centrosomal localization does not require microtubules, suggesting that DdCP224 is a genuine centrosomal component. DdCP224 exhibits sequence identity to a weakly conserved class of microtubule-associated proteins including human TOGp and yeast Stu2p. Stu2p has a size of only approximately 100 kDa and corresponds to the N-terminal half of DdCP224. The functions of the N- and C-terminal halves of DdCP224 were investigated in the corresponding GFP-fusion mutants. Surprisingly, the N-terminal construct showed only cytosolic localization, whereas the C-terminal construct localized exclusively to the centrosome. This is unexpected because Stu2p is localized at the spindle pole body. Full-length DdCP224-GFP was present both at centrosomes and along microtubules. Furthermore, it bound to microtubules in vitro, unlike the two truncated mutants. Thus centrosome binding is determined by the C-terminal half and microtubule binding may require the interaction of the N- and C-terminal halves. Interestingly, cells expressing full-length DdCP224-GFP exhibit supernumerary centrosomes and show a cytokinesis defect, suggesting that DdCP224 plays an important role in centrosome duplication. These features are unique among the known centrosomal proteins.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  R. Blau-Wasser, U. Euteneuer, H. Xiong, B. Gassen, M. Schleicher, and A. A. Noegel CP250, a Novel Acidic Coiled Coil Protein of the Dictyostelium centrosome, Affects Growth, Chemotaxis, and the Nuclear Envelope Mol. Biol. Cell, October 15, 2009; 20(20): 4348 - 4361. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Mana-Capelli, R. Graf, and D. A. Larochelle Dictyostelium discoideum CenB Is a Bona Fide Centrin Essential for Nuclear Architecture and Centrosome Stability Eukaryot. Cell, August 1, 2009; 8(8): 1106 - 1117. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Li, Q. Chen, M. Kaller, W. Nellen, R. Graf, and A. De Lozanne Dictyostelium Aurora Kinase Has Properties of both Aurora A and Aurora B Kinases Eukaryot. Cell, May 1, 2008; 7(5): 894 - 905. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Bonfils, N. Bec, B. Lacroix, M.-C. Harricane, and C. Larroque Kinetic Analysis of Tubulin Assembly in the Presence of the Microtubule-associated Protein TOGp J. Biol. Chem., February 23, 2007; 282(8): 5570 - 5581. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Khaire, R. Muller, R. Blau-Wasser, L. Eichinger, M. Schleicher, M. Rief, T. A. Holak, and A. A. Noegel Filamin-regulated F-actin Assembly Is Essential for Morphogenesis and Controls Phototaxis in Dictyostelium J. Biol. Chem., January 19, 2007; 282(3): 1948 - 1955. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Al-Bassam, M. van Breugel, S. C. Harrison, and A. Hyman Stu2p binds tubulin and undergoes an open-to-closed conformational change. J. Cell Biol., March 27, 2006; 172(7): 1009 - 1022. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kaller, U. Euteneuer, and W. Nellen Differential Effects of Heterochromatin Protein 1 Isoforms on Mitotic Chromosome Distribution and Growth in Dictyostelium discoideum Eukaryot. Cell, March 1, 2006; 5(3): 530 - 543. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Chen, H. Li, and A. De Lozanne Contractile Ring-independent Localization of DdINCENP, a Protein Important for Spindle Stability and Cytokinesis Mol. Biol. Cell, February 1, 2006; 17(2): 779 - 788. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Rehberg, J. Kleylein-Sohn, J. Faix, T.-H. Ho, I. Schulz, and R. Graf Dictyostelium LIS1 Is a Centrosomal Protein Required for Microtubule/Cell Cortex Interactions, Nucleus/Centrosome Linkage, and Actin Dynamics Mol. Biol. Cell, June 1, 2005; 16(6): 2759 - 2771. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Hamada, H. Igarashi, T. J. Itoh, T. Shimmen, and S. Sonobe Characterization of a 200 kDa Microtubule-associated Protein of Tobacco BY-2 Cells, a Member of the XMAP215/MOR1 Family Plant Cell Physiol., September 15, 2004; 45(9): 1233 - 1242. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Cassimeris and J. Morabito TOGp, the Human Homolog of XMAP215/Dis1, Is Required for Centrosome Integrity, Spindle Pole Organization, and Bipolar Spindle Assembly Mol. Biol. Cell, April 1, 2004; 15(4): 1580 - 1590. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Graf, U. Euteneuer, T.-H. Ho, and M. Rehberg Regulated Expression of the Centrosomal Protein DdCP224 Affects Microtubule Dynamics and Reveals Mechanisms for the Control of Supernumerary Centrosome Number Mol. Biol. Cell, October 1, 2003; 14(10): 4067 - 4074. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Rehberg and R. Graf Dictyostelium EB1 Is a Genuine Centrosomal Component Required for Proper Spindle Formation Mol. Biol. Cell, July 1, 2002; 13(7): 2301 - 2310. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Yasuhara, M. Muraoka, H. Shogaki, H. Mori, and S. Sonobe TMBP200, a Microtubule Bundling Polypeptide Isolated from Telophase Tobacco BY-2 Cells is a MOR1 Homologue Plant Cell Physiol., June 15, 2002; 43(6): 595 - 603. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Cassimeris, D. Gard, P. T. Tran, and H. P. Erickson XMAP215 is a long thin molecule that does not increase microtubule stiffness J. Cell Sci., March 10, 2002; 114(16): 3025 - 3033. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Graf DdNek2, the first non-vertebrate homologue of human Nek2, is involved in the formation of microtubule-organizing centers J. Cell Sci., January 5, 2002; 115(9): 1919 - 1929. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. O. Wasteneys Microtubule organization in the green kingdom: chaos or self-order? J. Cell Sci., January 4, 2002; 115(7): 1345 - 1354. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Kinoshita, I. Arnal, A. Desai, D. N. Drechsel, and A. A. Hyman Reconstitution of Physiological Microtubule Dynamics Using Purified Components Science, November 9, 2001; 294(5545): 1340 - 1343. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.-P. Janssen, R. Rost, L. Eichinger, and M. Schleicher Characterization of CD36/LIMPII Homologues in Dictyostelium discoideum J. Biol. Chem., October 12, 2001; 276(42): 38899 - 38910. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Severin, B. Habermann, T. Huffaker, and T. Hyman Stu2 Promotes Mitotic Spindle Elongation in Anaphase J. Cell Biol., April 16, 2001; 153(2): 435 - 442. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Ohkura, M. A. Garcia, and T. Toda Dis1/TOG universal microtubule adaptors - one MAP for all? J. Cell Sci., January 11, 2001; 114(21): 3805 - 3812. [Abstract] [Full Text] [PDF]
|
|
