|
|
|
|
|
||
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 113, Issue 11 1891-1902, Copyright © 2000 by Company of Biologists
JOURNAL ARTICLES |
PL McNeil, SS Vogel, K Miyake and M Terasaki
Department of Cellular Biology and Anatomy, and Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA, USA. pmcneil@mail.mcg.edu.
Vesicle-vesicle fusion initiated in cell cytoplasm by high Ca(2+) can rapidly erect large membrane boundaries. These might be used as a 'patch' for resealing plasma membrane disruptions. Three central predictions of this 'patch' hypothesis are here established in sea urchin eggs. First, we show that surface markers for plasma membrane protein and lipid are initially absent over disruption sites after resealing is complete. Second, we demonstrate that resealing capacity is strongly dependent upon local availability of fusion competent cytoplasmic organelles, specifically the reserve or yolk granule. Lastly, we demonstrate that the reserve granule is capable of rapid (t(1/2) <1 second), Ca(2+)-regulated (high threshold) fusion capable of erecting large (>1000 &mgr;m(2)), continuous membrane boundaries. Production of patch vesicles for resealing may proceed by an 'emergency' fusion mechanism distinct from that utilized for the much slower, highly regulated, cytosol-requiring organelle-organelle fusion events typical of constitutive membrane trafficking pathways.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  V. Idone, C. Tam, J. W. Goss, D. Toomre, M. Pypaert, and N. W. Andrews Repair of injured plasma membrane by rapid Ca2+-dependent endocytosis J. Cell Biol., March 5, 2008; 180(5): 905 - 914. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. B. Ramos, K. Miranda, W. de Souza, D. M. P. Oliveira, A. P. C. A. Lima, M. H. F. Sorgine, and E. A. Machado Calcium-regulated fusion of yolk granules is important for yolk degradation during early embryogenesis of Rhodnius prolixus Stahl J. Exp. Biol., January 1, 2007; 210(1): 138 - 148. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. K. McNeil, U. Rescher, V. Gerke, and P. L. McNeil Requirement for Annexin A1 in Plasma Membrane Repair J. Biol. Chem., November 17, 2006; 281(46): 35202 - 35207. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. P. Rader, W. Song, H. Van Remmen, A. Richardson, and J. A. Faulkner Raising the antioxidant levels within mouse muscle fibres does not affect contraction-induced injury Exp Physiol, July 1, 2006; 91(4): 781 - 789. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Farkas, J. Lifshitz, and J. T. Povlishock Mechanoporation induced by diffuse traumatic brain injury: an irreversible or reversible response to injury? J. Neurosci., March 22, 2006; 26(12): 3130 - 3140. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. McNeil and P. L. McNeil Yolk granule tethering: a role in cell resealing and identification of several protein components J. Cell Sci., October 15, 2005; 118(20): 4701 - 4708. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. G Allen, N. P Whitehead, and E. W Yeung Mechanisms of stretch-induced muscle damage in normal and dystrophic muscle: role of ionic changes J. Physiol., September 15, 2005; 567(3): 723 - 735. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Fein and M. Terasaki Rapid Increase in Plasma Membrane Chloride Permeability during Wound Resealing in Starfish Oocytes J. Gen. Physiol., July 25, 2005; 126(2): 151 - 159. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. E. Vlahakis and R. D. Hubmayr Cellular Stress Failure in Ventilator-injured Lungs Am. J. Respir. Crit. Care Med., June 15, 2005; 171(12): 1328 - 1342. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. H. Doerr, O. Gajic, J. C. Berrios, S. Caples, M. Abdel, J. F. Lymp, and R. D. Hubmayr Hypercapnic Acidosis Impairs Plasma Membrane Wound Resealing in Ventilator-injured Lungs Am. J. Respir. Crit. Care Med., June 15, 2005; 171(12): 1371 - 1377. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Galbraith and M. Terasaki Controlled Damage in Thick Specimens by Multiphoton Excitation Mol. Biol. Cell, May 1, 2003; 14(5): 1808 - 1817. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. L. McNeil, K. Miyake, and S. S. Vogel The endomembrane requirement for cell surface repair PNAS, April 15, 2003; 100(8): 4592 - 4597. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Togo, J. M. Alderton, and R. A. Steinhardt Long-Term Potentiation of Exocytosis and Cell Membrane Repair in Fibroblasts Mol. Biol. Cell, January 1, 2003; 14(1): 93 - 106. [Abstract] [Full Text]
|
|
|
|
|
|
J. H. Henson, R. Nazarian, K. L. Schulberg, V. A. Trabosh, S. E. Kolnik, A. R. Burns, and K. J. McPartland Wound Closure in the Lamellipodia of Single Cells: Mediation by Actin Polymerization in the Absence of an Actomyosin Purse String Mol. Biol. Cell, March 1, 2002; 13(3): 1001 - 1014. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Marshall Sidereus Nuncius it ain't: Cells, Gels, and the Engines of Life J. Cell Sci., January 10, 2002; 115(19): 3717 - 3717. [Full Text] [PDF]
|
|
|
|
|
|
P. L. McNeil Repairing a torn cell surface: make way, lysosomes to the rescue J. Cell Sci., January 3, 2002; 115(5): 873 - 879. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Miyake, P. L. McNeil, K. Suzuki, R. Tsunoda, and N. Sugai An actin barrier to resealing J. Cell Sci., January 10, 2001; 114(19): 3487 - 3494. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. H. Kim, T. A. Klotchkova, and Y.-M. Kang Life without a cell membrane: regeneration of protoplasts from disintegrated cells of the marine green alga Bryopsis plumosa J. Cell Sci., January 6, 2001; 114(11): 2009 - 2014. [Abstract] [Full Text] [PDF]
|
|
