Journal of Cell Science, Vol 113, Issue 11 1939-1950, Copyright © 2000 by Company of Biologists

JOURNAL ARTICLES

HZwint-1, a novel human kinetochore component that interacts with HZW10

DA Starr, R Saffery, Z Li, AE Simpson, KH Choo, TJ Yen and ML Goldberg
Section of Genetics and Development, Cornell University, Ithaca, NY 14853, USA.

HZwint-1 (Human ZW10 interacting protein-1) was identified in a yeast two hybrid screen for proteins that interact with HZW10. HZwint-1 cDNA encodes a 43 kDa protein predicted to contain an extended coiled-coil domain. Immunofluorescence studies with sera raised against HZwint-1 protein revealed strong kinetochore staining in nocodazole-arrested chromosome spreads. This signal co-localizes at the kinetochore with HZW10, at a position slightly outside of the central part of the centromere as revealed by staining with a CREST serum. The kinetochore localization of HZwint-1 has been confirmed by following GFP fluorescence in HeLa cells transiently transfected with a plasmid encoding a GFP/HZwint-1 fusion protein. In cycling HeLa cells, HZwint-1 localizes to the kinetochore of prophase HeLa cells prior to HZW10 localization, and remains at the kinetochore until late in anaphase. This localization pattern, combined with the two-hybrid results, suggests that HZwint-1 may play a role in targeting HZW10 to the kinetochore at prometaphase. HZwint-1 was also found to localize to neocentromeres and to the active centromere of dicentric chromosomes. HZwint-1 thus appears to associate with all active centromeres, implying that it plays an important role in correct centromere function.

This article has been cited by other articles:

				J. K. Famulski, L. Vos, X. Sun, and G. Chan Stable hZW10 kinetochore residency, mediated by hZwint-1 interaction, is essential for the mitotic checkpoint J. Cell Biol., February 6, 2008; 180(3): 507 - 520. [Abstract] [Full Text] [PDF]

				S. A. Stehman, Y. Chen, R. J. McKenney, and R. B. Vallee NudE and NudEL are required for mitotic progression and are involved in dynein recruitment to kinetochores J. Cell Biol., August 9, 2007; 178(4): 583 - 594. [Abstract] [Full Text] [PDF]

				M. J. Emanuele, M. L. McCleland, D. L. Satinover, and P. T. Stukenberg Measuring the Stoichiometry and Physical Interactions between Components Elucidates the Architecture of the Vertebrate Kinetochore Mol. Biol. Cell, October 1, 2005; 16(10): 4882 - 4892. [Abstract] [Full Text] [PDF]

				G. J.P.L. Kops, Y. Kim, B. A.A. Weaver, Y. Mao, I. McLeod, J. R. Yates III, M. Tagaya, and D. W. Cleveland ZW10 links mitotic checkpoint signaling to the structural kinetochore J. Cell Biol., April 11, 2005; 169(1): 49 - 60. [Abstract] [Full Text] [PDF]

				G. D. Sgarlato, C. L. Eastman, and H. H. Sussman Panel of Genes Transcriptionally Up-regulated in Squamous Cell Carcinoma of the Cervix Identified by Representational Difference Analysis, Confirmed by Macroarray, and Validated by Real-Time Quantitative Reverse Transcription-PCR Clin. Chem., January 1, 2005; 51(1): 27 - 34. [Abstract] [Full Text] [PDF]

				H. Wang, X. Hu, X. Ding, Z. Dou, Z. Yang, A. W. Shaw, M. Teng, D. W. Cleveland, M. L. Goldberg, L. Niu, et al. Human Zwint-1 Specifies Localization of Zeste White 10 to Kinetochores and Is Essential for Mitotic Checkpoint Signaling J. Biol. Chem., December 24, 2004; 279(52): 54590 - 54598. [Abstract] [Full Text] [PDF]

				I. M. Cheeseman, S. Niessen, S. Anderson, F. Hyndman, J. R. Yates III, K. Oegema, and A. Desai A conserved protein network controls assembly of the outer kinetochore and its ability to sustain tension Genes & Dev., September 15, 2004; 18(18): 2255 - 2268. [Abstract] [Full Text] [PDF]

				H. Endoh, S. Tomida, Y. Yatabe, H. Konishi, H. Osada, K. Tajima, H. Kuwano, T. Takahashi, and T. Mitsudomi Prognostic Model of Pulmonary Adenocarcinoma by Expression Profiling of Eight Genes As Determined by Quantitative Real-Time Reverse Transcriptase Polymerase Chain Reaction J. Clin. Oncol., March 1, 2004; 22(5): 811 - 819. [Abstract] [Full Text] [PDF]

				A. Rose, S. Manikantan, S. J. Schraegle, M. A. Maloy, E. A. Stahlberg, and I. Meier Genome-Wide Identification of Arabidopsis Coiled-Coil Proteins and Establishment of the ARABI-COIL Database Plant Physiology, March 1, 2004; 134(3): 927 - 939. [Abstract] [Full Text] [PDF]

				A. Saxena, L. H. Wong, P. Kalitsis, E. Earle, L. G. Shaffer, and K.H. A. Choo Poly(ADP-ribose) polymerase 2 localizes to mammalian active centromeres and interacts with PARP-1, Cenpa, Cenpb and Bub3, but not Cenpc Hum. Mol. Genet., September 15, 2002; 11(19): 2319 - 2329. [Abstract] [Full Text] [PDF]

				G. J. Poggioli, R. L. DeBiasi, R. Bickel, R. Jotte, A. Spalding, G. L. Johnson, and K. L. Tyler Reovirus-Induced Alterations in Gene Expression Related to Cell Cycle Regulation J. Virol., February 22, 2002; 76(6): 2585 - 2594. [Abstract] [Full Text] [PDF]

				A. A. Van Hooser, I. I. Ouspenski, H. C. Gregson, D. A. Starr, T. J. Yen, M. L. Goldberg, K. Yokomori, W. C. Earnshaw, K. F. Sullivan, and B. R. Brinkley Specification of kinetochore-forming chromatin by the histone H3 variant CENP-A J. Cell Sci., January 10, 2001; 114(19): 3529 - 3542. [Abstract] [Full Text] [PDF]

				F. Scaerou, D. A. Starr, F. Piano, O. Papoulas, R. E. Karess, and M. L. Goldberg The ZW10 and Rough Deal checkpoint proteins function together in a large, evolutionarily conserved complex targeted to the kinetochore J. Cell Sci., January 9, 2001; 114(17): 3103 - 3114. [Abstract] [Full Text] [PDF]

				N. Sugata, S. Li, W. C. Earnshaw, T. J. Yen, K. Yoda, H. Masumoto, E. Munekata, P. E. Warburton, and K. Todokoro Human CENP-H multimers colocalize with CENP-A and CENP-C at active centromere-kinetochore complexes Hum. Mol. Genet., November 1, 2000; 9(19): 2919 - 2926. [Abstract] [Full Text] [PDF]