spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Naylor, S.
Right arrow Articles by Dale, T. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Naylor, S.
Right arrow Articles by Dale, T. C.

Journal of Cell Science, Vol 113, Issue 12 2129-2138, Copyright © 2000 by Company of Biologists

JOURNAL ARTICLES

Retroviral expression of Wnt-1 and Wnt-7b produces different effects in mouse mammary epithelium

S Naylor, MJ Smalley, D Robertson, BA Gusterson, PA Edwards and TC Dale
Developmental Biology, Section of Cell Biology and Experimental Pathology, Toby Robins Breakthrough Breast Cancer Research Centre, Institute of Cancer Research, London, SW3 6JB, UK.

Several Wnt genes are expressed in the postnatal mouse mammary gland and are thought to be involved in mammary gland development. Ectopic expression of Wnt-1, which is not normally expressed in the mammary gland, drives the formation of a pre-neoplastic hyperplasia. Cell culture-based assays have shown that Wnt-1 and some mammary-expressed Wnts transform C57MG cells. This has led to the suggestion that Wnt-1 functions as an oncogene through the inappropriate activation of developmental events that are normally controlled by the 'transforming' class of Wnts. In this study, Wnt-7b was expressed in vivo using recombinant retroviruses. Wnt-7b did not alter normal mammary gland development despite having similar effects to Wnt-1 in cell culture. We conclude that the in vitro classification of Wnts as 'transforming' does not correlate with the transformation in vivo. To facilitate the analysis of Wnt-expression, a lacZ-containing, bicistronic recombinant retrovirus was developed. Immunohistochemistry and electron microscopy identified retrovirally transduced myoepithelial and luminal epithelial cells in normal and hyperplastic tissues. The distribution of transduced cells in mammary outgrowths was consistent with current models of mammary stem cell identity.


This article has been cited by other articles:


Home page
 EndocrinologyHome page
A. Kuorelahti, S. Rulli, I. Huhtaniemi, and M. Poutanen
Human Chorionic Gonadotropin (hCG) Up-Regulates wnt5b and wnt7b in the Mammary Gland, and hCG{beta} Transgenic Female Mice Present with Mammary Gland Tumors Exhibiting Characteristics of the Wnt/{beta}-Catenin Pathway Activation
Endocrinology, August 1, 2007; 148(8): 3694 - 3703.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
J. Harris, P. M. Stanford, K. Sutherland, S. R. Oakes, M. J. Naylor, F. G. Robertson, K. D. Blazek, M. Kazlauskas, H. N. Hilton, S. Wittlin, et al.
Socs2 and Elf5 Mediate Prolactin-Induced Mammary Gland Development
Mol. Endocrinol., May 1, 2006; 20(5): 1177 - 1187.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
J. W. Jonker, J. Freeman, E. Bolscher, S. Musters, A. J. Alvi, I. Titley, A. H. Schinkel, and T. C. Dale
Contribution of the ABC Transporters Bcrp1 and Mdr1a/1b to the Side Population Phenotype in Mammary Gland and Bone Marrow of Mice
Stem Cells, September 1, 2005; 23(8): 1059 - 1065.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
A. Mudher, S. Chapman, J. Richardson, A. Asuni, G. Gibb, C. Pollard, R. Killick, T. Iqbal, L. Raymond, I. Varndell, et al.
Dishevelled Regulates the Metabolism of Amyloid Precursor Protein via Protein Kinase C/Mitogen-Activated Protein Kinase and c-Jun Terminal Kinase
J. Neurosci., July 15, 2001; 21(14): 4987 - 4995.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2000