|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 113, Issue 12 2233-2242, Copyright © 2000 by Company of Biologists
JOURNAL ARTICLES |
K Schmidt, H Dartsch, D Linder, HF Kern and R Kleene
Institut fur Zytobiologie und Zytopathologie, Philipps Universitat, Robert-Koch-Str. 5, D-35033 Marburg, Germany.
The secretory lectin ZG16p mediated the binding of aggregated zymogens to the granule membrane in pancreatic acinar cells. Using a recently established in vitro condensation-sorting assay, we now show that pretreatment of zymogen granule membranes (ZGM) with either sodium bicarbonate at pH 10 or with phosphatidyl inositol-specific phospholipase C (PI-PLC) reduced the binding efficiency of zymogens to the same extent, as distinct components were liberated from ZGM. Analysis of the composition of the bicarbonate extract revealed the presence of the secretory lectin ZG16p, the serpin ZG46p and the GPI-linked glycoprotein GP-2, together with several unknown proteins, and small amounts of lipase and carboxylester lipase. The unknown proteins detected in 2-D gels represented a group of acidic and basic protein spots, which were positive in a glycan staining reaction and were soluble in methanol. One protein spot of the acidic group and several of the basic group reacted with a monoclonal antibody directed against chondroitin sulfate, indicating that the proteins represented proteoglycans. A staining pattern similar to the glycan reaction was observed in immunoblots using a polyclonal antibody directed against the whole bicarbonate extract. Immunogold electron microscopy revealed that this antibody reacted with components in the periphery of zymogen granules and strongly stained ZGM in the pellet fraction of a standard in vitro condensation-sorting assay. The amino acid composition of isolated components of both the acidic and basic group showed similarities to aggrecan, a cartilage-specific proteoglycan, and to glycine-rich glycoproteins, respectively. We therefore conclude that a submembranous matrix on the ZGM composed of proteoglycans and glycoproteins is involved in granule formation in pancreatic acinar cells.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  X. Chen, P. J. Ulintz, E. S. Simon, J. A. Williams, and P. C. Andrews Global Topology Analysis of Pancreatic Zymogen Granule Membrane Proteins Mol. Cell. Proteomics, December 1, 2008; 7(12): 2323 - 2336. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. von Lupke, G. Schauermann, I. Feussner, and G. Hinz Peripheral membrane proteins mediate binding of vacuolar storage proteins to membranes of the secretory pathway of developing pea cotyledons J. Exp. Bot., April 1, 2008; 59(6): 1327 - 1340. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Yu, S. A. Michie, and A. W. Lowe Absence of the Major Zymogen Granule Membrane Protein, GP2, Does Not Affect Pancreatic Morphology or Secretion J. Biol. Chem., November 26, 2004; 279(48): 50274 - 50279. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Boulatnikov and R. C. De Lisle Binding of the Golgi Sorting Receptor Muclin to Pancreatic Zymogens through Sulfated O-linked Oligosaccharides J. Biol. Chem., September 24, 2004; 279(39): 40918 - 40926. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. U. Niemann, J. B. Cowland, P. Klausen, J. Askaa, J. Calafat, and N. Borregaard Localization of serglycin in human neutrophil granulocytes and their precursors J. Leukoc. Biol., August 1, 2004; 76(2): 406 - 415. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Lemansky, M. Gerecitano-Schmidek, R. C. Das, B. Schmidt, and A. Hasilik Targeting myeloperoxidase to azurophilic granules in HL-60 cells J. Leukoc. Biol., October 1, 2003; 74(4): 542 - 550. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. G. Venkatesh and S.-U. Gorr A sulfated proteoglycan is necessary for storage of exocrine secretory proteins in the rat parotid gland Am J Physiol Cell Physiol, August 1, 2002; 283(2): C438 - C445. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. C. De Lisle Role of sulfated O-linked glycoproteins in zymogen granule formation J. Cell Sci., July 15, 2002; 115(14): 2941 - 2952. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L Vayssie, N Garreau de Loubresse, and L Sperling Growth and form of secretory granules involves stepwise assembly but not differential sorting of a family of secretory proteins in Paramecium J. Cell Sci., January 3, 2001; 114(5): 875 - 886. [Abstract] [PDF]
|
|
|
|
|
|
K. Schmidt, M. Schrader, H.-F. Kern, and R. Kleene Regulated Apical Secretion of Zymogens in Rat Pancreas. INVOLVEMENT OF THE GLYCOSYLPHOSPHATIDYLINOSITOL-ANCHORED GLYCOPROTEIN GP-2, THE LECTIN ZG16p, AND CHOLESTEROL-GLYCOSPHINGOLIPID-ENRICHED MICRODOMAINS J. Biol. Chem., April 20, 2001; 276(17): 14315 - 14323. [Abstract] [Full Text] [PDF]
|
|
