spacer gif spacer gif spacer gif spacer gif Propose a workshop for 2011 spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hoogenraad, C. C.
Right arrow Articles by Galjart, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hoogenraad, C. C.
Right arrow Articles by Galjart, N.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Journal of Cell Science, Vol 113, Issue 12 2285-2297, Copyright © 2000 by Company of Biologists

JOURNAL ARTICLES

Functional analysis of CLIP-115 and its binding to microtubules

CC Hoogenraad, A Akhmanova, F Grosveld, CI De Zeeuw and N Galjart
MGC Department of Cell Biology and Genetics and Department of Anatomy, Erasmus University, PO Box 1738, The Netherlands.

Cytoplasmic linker proteins (CLIPs) bind to microtubules and are proposed to link this cytoskeletal network to other intracellular structures. We are interested in CLIP-115, since this protein is enriched in neuronal dendrites and may operate in the control of brain-specific organelle translocations. Each CLIP monomer is characterized by two microtubule-binding (MTB) motifs, surrounded by basic, serine-rich regions. This head domain is connected to the C-terminal tail through a long coiled-coil structure. The MTB domains are conserved as a single domain in other proteins involved in microtubule based transport and dynamics, such as p150(Glued). Here we provide evidence that efficient binding of CLIP-115 to microtubules is sensitive to phosphorylation and is not mediated by the conserved MTB domains alone, but requires the presence of the basic, serine rich regions in addition to the MTB motifs. In transfected COS-1 cells, CLIP-115 initially accumulates at the distal ends of microtubules and coincides with CLIP-170, indicating that both proteins mark growing microtubule ends. However, when expressed at higher levels, CLIP-115 and -170 affect the microtubule network differently. This might be partly due to the divergent C-termini of the two proteins. We demonstrate that, similar to CLIP-170, CLIP-115 forms homodimers, which, at least in vitro, are linked by disulfide bridges. Cysteine(391) of CLIP-115, however, is specific in that it controls the microtubule bundling capacity of certain mutant CLIP-115 molecules. Therefore, both similar and specific mechanisms appear to regulate the conformation of CLIPs as well as their binding to microtubules.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
K. K. Gupta, B. A. Paulson, E. S. Folker, B. Charlebois, A. J. Hunt, and H. V. Goodson
Minimal Plus-end Tracking Unit of the Cytoplasmic Linker Protein CLIP-170
J. Biol. Chem., March 13, 2009; 284(11): 6735 - 6742.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
Y. Komarova, C. O. De Groot, I. Grigoriev, S. M. Gouveia, E. L. Munteanu, J. M. Schober, S. Honnappa, R. M. Buey, C. C. Hoogenraad, M. Dogterom, et al.
Mammalian end binding proteins control persistent microtubule growth
J. Cell Biol., March 9, 2009; 184(5): 691 - 706.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
E. Lewkowicz, F. Herit, C. Le Clainche, P. Bourdoncle, F. Perez, and F. Niedergang
The microtubule-binding protein CLIP-170 coordinates mDia1 and actin reorganization during CR3-mediated phagocytosis
J. Cell Biol., December 29, 2008; 183(7): 1287 - 1298.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
J. Hendriksen, M. Jansen, C. M. Brown, H. van der Velde, M. van Ham, N. Galjart, G. J. Offerhaus, F. Fagotto, and M. Fornerod
Plasma membrane recruitment of dephosphorylated {beta}-catenin upon activation of the Wnt pathway
J. Cell Sci., June 1, 2008; 121(11): 1793 - 1802.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
K. A. Dragestein, W. A. van Cappellen, J. van Haren, G. D. Tsibidis, A. Akhmanova, T. A. Knoch, F. Grosveld, and N. Galjart
Dynamic behavior of GFP-CLIP-170 reveals fast protein turnover on microtubule plus ends
J. Cell Biol., February 25, 2008; 180(4): 729 - 737.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. Hoppeler-Lebel, C. Celati, G. Bellett, M. M. Mogensen, L. Klein-Hitpass, M. Bornens, and A.-M. Tassin
Centrosomal CAP350 protein stabilises microtubules associated with the Golgi complex
J. Cell Sci., September 15, 2007; 120(18): 3299 - 3308.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
E. Teuling, S. Ahmed, E. Haasdijk, J. Demmers, M. O. Steinmetz, A. Akhmanova, D. Jaarsma, and C. C. Hoogenraad
Motor Neuron Disease-Associated Mutant Vesicle-Associated Membrane Protein-Associated Protein (VAP) B Recruits Wild-Type VAPs into Endoplasmic Reticulum-Derived Tubular Aggregates
J. Neurosci., September 5, 2007; 27(36): 9801 - 9815.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
Y. Jeong, J. Lee, K. Kim, J. C. Yoo, and K. Rhee
Characterization of NIP2/centrobin, a novel substrate of Nek2, and its potential role in microtubule stabilization
J. Cell Sci., June 15, 2007; 120(12): 2106 - 2116.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
J. M. Schober, Y. A. Komarova, O. Y. Chaga, A. Akhmanova, and G. G. Borisy
Microtubule-targeting-dependent reorganization of filopodia
J. Cell Sci., April 1, 2007; 120(7): 1235 - 1244.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
L. Peris, M. Thery, J. Faure, Y. Saoudi, L. Lafanechere, J. K. Chilton, P. Gordon-Weeks, N. Galjart, M. Bornens, L. Wordeman, et al.
Tubulin tyrosination is a major factor affecting the recruitment of CAP-Gly proteins at microtubule plus ends
J. Cell Biol., September 11, 2006; 174(6): 839 - 849.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
J. R. Levy, C. J. Sumner, J. P. Caviston, M. K. Tokito, S. Ranganathan, L. A. Ligon, K. E. Wallace, B. H. LaMonte, G. G. Harmison, I. Puls, et al.
A motor neuron disease-associated mutation in p150Glued perturbs dynactin function and induces protein aggregation
J. Cell Biol., February 27, 2006; 172(5): 733 - 745.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
X. Yan, R. Habedanck, and E. A. Nigg
A Complex of Two Centrosomal Proteins, CAP350 and FOP, Cooperates with EB1 in Microtubule Anchoring
Mol. Biol. Cell, February 1, 2006; 17(2): 634 - 644.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
Y. Komarova, G. Lansbergen, N. Galjart, F. Grosveld, G. G. Borisy, and A. Akhmanova
EB1 and EB3 Control CLIP Dissociation from the Ends of Growing Microtubules
Mol. Biol. Cell, November 1, 2005; 16(11): 5334 - 5345.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
A. Akhmanova, A.-L. Mausset-Bonnefont, W. van Cappellen, N. Keijzer, C. C. Hoogenraad, T. Stepanova, K. Drabek, J. van der Wees, M. Mommaas, J. Onderwater, et al.
The microtubule plus-end-tracking protein CLIP-170 associates with the spermatid manchette and is essential for spermatogenesis
Genes & Dev., October 15, 2005; 19(20): 2501 - 2515.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
L. D. Kosturko, M. J. Maggipinto, C. D'Sa, J. H. Carson, and E. Barbarese
The Microtubule-associated Protein Tumor Overexpressed Gene Binds to the RNA Trafficking Protein Heterogeneous Nuclear Ribonucleoprotein A2
Mol. Biol. Cell, April 1, 2005; 16(4): 1938 - 1947.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
C. Vedrenne, D. R. Klopfenstein, and H.-P. Hauri
Phosphorylation Controls CLIMP-63-mediated Anchoring of the Endoplasmic Reticulum to Microtubules
Mol. Biol. Cell, April 1, 2005; 16(4): 1928 - 1937.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. A. Farah, D. Liazoghli, S. Perreault, M. Desjardins, A. Guimont, A. Anton, M. Lauzon, G. Kreibich, J. Paiement, and N. Leclerc
Interaction of Microtubule-associated Protein-2 and p63: A NEW LINK BETWEEN MICROTUBULES AND ROUGH ENDOPLASMIC RETICULUM MEMBRANES IN NEURONS
J. Biol. Chem., March 11, 2005; 280(10): 9439 - 9449.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
G. Guarguaglini, P. I. Duncan, Y. D. Stierhof, T. Holmstrom, S. Duensing, and E. A. Nigg
The Forkhead-associated Domain Protein Cep170 Interacts with Polo-like Kinase 1 and Serves as a Marker for Mature Centrioles
Mol. Biol. Cell, March 1, 2005; 16(3): 1095 - 1107.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
G. Lansbergen, Y. Komarova, M. Modesti, C. Wyman, C. C. Hoogenraad, H. V. Goodson, R. P. Lemaitre, D. N. Drechsel, E. van Munster, T. W.J. Gadella Jr., et al.
Conformational changes in CLIP-170 regulate its binding to microtubules and dynactin localization
J. Cell Biol., October 4, 2004; (2004) jcb.200402082.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. Lallemand-Breitenbach, M. Quesnoit, V. Braun, A. El Marjou, C. Pous, B. Goud, and F. Perez
CLIPR-59 Is a Lipid Raft-associated Protein Containing a Cytoskeleton-associated Protein Glycine-rich Domain (CAP-Gly) That Perturbs Microtubule Dynamics
J. Biol. Chem., September 24, 2004; 279(39): 41168 - 41178.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
W. Bu and L.-K. Su
Characterization of Functional Domains of Human EB1 Family Proteins
J. Biol. Chem., December 12, 2003; 278(50): 49721 - 49731.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
M. Tassabehji
Williams-Beuren syndrome: a challenge for genotype-phenotype correlations
Hum. Mol. Genet., October 15, 2003; 12(90002): R229 - 237.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
T. Stepanova, J. Slemmer, C. C. Hoogenraad, G. Lansbergen, B. Dortland, C. I. De Zeeuw, F. Grosveld, G. van Cappellen, A. Akhmanova, and N. Galjart
Visualization of Microtubule Growth in Cultured Neurons via the Use of EB3-GFP (End-Binding Protein 3-Green Fluorescent Protein)
J. Neurosci., April 1, 2003; 23(7): 2655 - 2664.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
Y. A. Komarova, A. S. Akhmanova, S.-i. Kojima, N. Galjart, and G. G. Borisy
Cytoplasmic linker proteins promote microtubule rescue in vivo
J. Cell Biol., November 25, 2002; 159(4): 589 - 599.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
J. M. Askham, K. T. Vaughan, H. V. Goodson, and E. E. Morrison
Evidence That an Interaction between EB1 and p150Glued Is Required for the Formation and Maintenance of a Radial Microtubule Array Anchored at the Centrosome
Mol. Biol. Cell, October 1, 2002; 13(10): 3627 - 3645.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
P. S. Vaughan, P. Miura, M. Henderson, B. Byrne, and K. T. Vaughan
A role for regulated binding of p150Glued to microtubule plus ends in organelle transport
J. Cell Biol., July 22, 2002; 158(2): 305 - 319.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
F. M. Coquelle, M. Caspi, F. P. Cordelieres, J. P. Dompierre, D. L. Dujardin, C. Koifman, P. Martin, C. C. Hoogenraad, A. Akhmanova, N. Galjart, et al.
LIS1, CLIP-170's Key to the Dynein/Dynactin Pathway
Mol. Cell. Biol., May 1, 2002; 22(9): 3089 - 3102.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
Y. A. Komarova, I. A. Vorobjev, and G. G. Borisy
Life cycle of MTs: persistent growth in the cell interior, asymmetric transition frequencies and effects of the cell boundary
J. Cell Sci., January 9, 2002; 115(17): 3527 - 3539.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
I. Kaverina, O. Krylyshkina, K. Beningo, K. Anderson, Y.-L. Wang, and J. V. Small
Tensile stress stimulates microtubule outgrowth in living cells
J. Cell Sci., January 6, 2002; 115(11): 2283 - 2291.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2000