spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wong, K. C.
Right arrow Articles by Balasubramanian, M. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wong, K. C.
Right arrow Articles by Balasubramanian, M. K.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Journal of Cell Science, Vol 113, Issue 13 2421-2432, Copyright © 2000 by Company of Biologists

JOURNAL ARTICLES

Fission yeast Rng3p: an UCS-domain protein that mediates myosin II assembly during cytokinesis

KC Wong, NI Naqvi, Y Iino, M Yamamoto and MK Balasubramanian
Cell Division Laboratory, Institute of Molecular Agrobiology, The National University of Singapore, Singapore 117604, Republic of Singapore.

Cell division in many eukaryotes, including the fission yeast Schizosaccharomyces pombe, utilizes a contractile actomyosin ring. In S. pombe, the actomyosin ring is assembled at the medial cortex upon entry into mitosis and constricts at the end of anaphase to guide the centripetal deposition of the septum. Despite identification of several structural components essential for actomyosin ring assembly, the interdependencies between these gene-products in the process of ring assembly are unknown. This study investigates the role of Rng3p, a member of the UCS-domain containing protein family (Unc-45p, Cro1p, She4p), in actomyosin ring assembly. Null mutants in rng3 resemble deletion mutants in the type II myosin heavy chain (myo2) and rng3(ts) mutants show strong negative interactions with the myo2-E1 mutant, suggesting that Rng3p is involved in modulating aspects of type II myosin function. Interestingly, a green fluorescent protein (GFP) tagged Rng3p fusion is detected at the division site in the myo2-E1 mutant, but not in other myo2-alleles, wild-type cells or in 18 other cytokinesis mutants. Assembly and maintenance of Rng3p at the division site in the myo2-E1 mutant requires F-actin. Rng3p is also required for the proper assembly of Myo2p and F-actin into a functional actomyosin ring but is not necessary for their accumulation at the division site. We conclude that Rng3p is a novel component of the F-actin cytoskeleton essential for a late step in actomyosin ring assembly and that it might monitor some aspect of type II myosin assembly during actomyosin ring construction.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
R. Satoh, T. Morita, H. Takada, A. Kita, S. Ishiwata, A. Doi, K. Hagihara, A. Taga, Y. Matsumura, H. Tohda, et al.
Role of the RNA-binding Protein Nrd1 and Pmk1 Mitogen-activated Protein Kinase in the Regulation of Myosin mRNA Stability in Fission Yeast
Mol. Biol. Cell, May 1, 2009; 20(9): 2473 - 2485.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
H. Yan, W. Ge, T. G. Chew, J. Y. Chow, D. McCollum, A. M. Neiman, and M. K. Balasubramanian
The Meiosis-Specific Sid2p-related Protein Slk1p Regulates Forespore Membrane Assembly in Fission Yeast
Mol. Biol. Cell, September 1, 2008; 19(9): 3676 - 3690.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Lord, T. E. Sladewski, and T. D. Pollard
Yeast UCS proteins promote actomyosin interactions and limit myosin turnover in cells
PNAS, June 10, 2008; 105(23): 8014 - 8019.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Liu, R. Srikakulam, and D. A. Winkelmann
Unc45 Activates Hsp90-dependent Folding of the Myosin Motor Domain
J. Biol. Chem., May 9, 2008; 283(19): 13185 - 13193.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
C. Etard, U. Roostalu, and U. Strahle
Shuttling of the chaperones Unc45b and Hsp90a between the A band and the Z line of the myofibril
J. Cell Biol., March 24, 2008; 180(6): 1163 - 1175.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
M. Lord, E. Laves, and T. D. Pollard
Cytokinesis Depends on the Motor Domains of Myosin-II in Fission Yeast but Not in Budding Yeast
Mol. Biol. Cell, November 1, 2005; 16(11): 5346 - 5355.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
M. Mishra, V. M. D'souza, K. C. Chang, Y. Huang, and M. K. Balasubramanian
Hsp90 Protein in Fission Yeast Swo1p and UCS Protein Rng3p Facilitate Myosin II Assembly and Function
Eukaryot. Cell, March 1, 2005; 4(3): 567 - 576.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
J. Karagiannis, A. Bimbo, S. Rajagopalan, J. Liu, and M. K. Balasubramanian
The Nuclear Kinase Lsk1p Positively Regulates the Septation Initiation Network and Promotes the Successful Completion of Cytokinesis in Response to Perturbation of the Actomyosin Ring in Schizosaccharomyces pombe
Mol. Biol. Cell, January 1, 2005; 16(1): 358 - 371.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
M. Lord and T. D. Pollard
UCS protein Rng3p activates actin filament gliding by fission yeast myosin-II
J. Cell Biol., October 25, 2004; 167(2): 315 - 325.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
T. Kachur, W. Ao, J. Berger, and D. Pilgrim
Maternal UNC-45 is involved in cytokinesis and colocalizes with non-muscle myosin in the early Caenorhabditis elegans embryo
J. Cell Sci., October 15, 2004; 117(22): 5313 - 5321.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
F. Motegi, M. Mishra, M. K. Balasubramanian, and I. Mabuchi
Myosin-II reorganization during mitosis is controlled temporally by its dephosphorylation and spatially by Mid1 in fission yeast
J. Cell Biol., June 7, 2004; 165(5): 685 - 695.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
H. Toi, K. Fujimura-Kamada, K. Irie, Y. Takai, S. Todo, and K. Tanaka
She4p/Dim1p Interacts with the Motor Domain of Unconventional Myosins in the Budding Yeast, Saccharomyces cerevisiae
Mol. Biol. Cell, June 1, 2003; 14(6): 2237 - 2249.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
J. Karagiannis, R. Oulton, and P. G. Young
The Scw1 RNA-Binding Domain Protein Regulates Septation and Cell-Wall Structure in Fission Yeast
Genetics, September 1, 2002; 162(1): 45 - 58.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. H. Hutagalung, M. L. Landsverk, M. G. Price, and H. F. Epstein
The UCS family of myosin chaperones
J. Cell Sci., January 11, 2002; 115(21): 3983 - 3990.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
M. G. Price, M. L. Landsverk, J. M. Barral, and H. F. Epstein
Two mammalian UNC-45 isoforms are related to distinct cytoskeletal and muscle-specific functions
J. Cell Sci., January 11, 2002; 115(21): 4013 - 4023.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
H. Wang, X. Tang, J. Liu, S. Trautmann, D. Balasundaram, D. McCollum, and M. K. Balasubramanian
The Multiprotein Exocyst Complex Is Essential for Cell Separation in Schizosaccharomyces pombe
Mol. Biol. Cell, February 1, 2002; 13(2): 515 - 529.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2000