|
|
|
|
|
||
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 113, Issue 14 2607-2618, Copyright © 2000 by Company of Biologists
JOURNAL ARTICLES |
S Lecat, P Verkade, C Thiele, K Fiedler, K Simons and F Lafont
Cell Biology and Biophysics Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, D-69117 Heidelberg, Germany.
Annexins form a family of proteins that are widely expressed and known to bind membranes in the presence of calcium. Two isoforms of the annexin XIII subfamily are expressed in epithelia. We previously reported that annexin XIIIb is apically localized in MDCK cells and that it is involved in raft-mediated delivery of apical proteins. We have now analyzed the properties of annexin XIIIa, which differs from annexin XIIIb by a deletion of 41 amino acids in the amino-terminal domain, and is distributed both apically and basolaterally. Annexin XIIIa binding to membranes is independent of calcium but requires its myristoyl amino-terminal modification, as observed with annexin XIIIb. Our biochemical and functional data show that annexin XIIIa behaves differently in the apical and in the basolateral compartments. Whereas annexin XIIIa apically can associate with rafts independently of calcium, the basolateral pool requires calcium for this. Annexin XIIIa, like annexin XIIIb, stimulates apical transport of influenza virus hemagglutinin but, in contrast, only annexin XIIIa inhibits basolateral transport of vesicular stomatitis virus G protein. Our results suggest that annexin XIIIa and XIIIb have specific roles in epithelial cells, and because of their structural similarities, these isoforms offer interesting tools for unravelling the functions of annexins.
This article has been cited by other articles:
|
|
 |
|
  K. Okamoto, Y. Mori, Y. Komoda, T. Okamoto, M. Okochi, M. Takeda, T. Suzuki, K. Moriishi, and Y. Matsuura Intramembrane Processing by Signal Peptide Peptidase Regulates the Membrane Localization of Hepatitis C Virus Core Protein and Viral Propagation J. Virol., September 1, 2008; 82(17): 8349 - 8361. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Cao, C. Li, J. N. Higginbotham, J. L. Franklin, D. L. Tabb, R. Graves-Deal, S. Hill, K. Cheek, W. G. Jerome, L. A. Lapierre, et al. Use of Fluorescence-activated Vesicle Sorting for Isolation of Naked2-associated, Basolaterally Targeted Exocytic Vesicles for Proteomics Analysis Mol. Cell. Proteomics, September 1, 2008; 7(9): 1651 - 1667. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Torkko, A. Manninen, S. Schuck, and K. Simons Depletion of apical transport proteins perturbs epithelial cyst formation and ciliogenesis J. Cell Sci., April 15, 2008; 121(8): 1193 - 1203. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Fischer, L. Lu, H. T. Haigler, and R. Langen Annexin B12 Is a Sensor of Membrane Curvature and Undergoes Major Curvature-dependent Structural Changes J. Biol. Chem., March 30, 2007; 282(13): 9996 - 10004. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Shmuel, L. C. Santy, S. Frank, D. Avrahami, J. E. Casanova, and Y. Altschuler ARNO through Its Coiled-coil Domain Regulates Endocytosis at the Apical Surface of Polarized Epithelial Cells J. Biol. Chem., May 12, 2006; 281(19): 13300 - 13308. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Cezanne, S. Lecat, B. Lagane, C. Millot, J.-Y. Vollmer, H. Matthes, J.-L. Galzi, and A. Lopez Dynamic Confinement of NK2 Receptors in the Plasma Membrane: IMPROVED FRAP ANALYSIS AND BIOLOGICAL RELEVANCE J. Biol. Chem., October 22, 2004; 279(43): 45057 - 45067. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Elazar, P. Liu, C. M. Rice, and J. S. Glenn An N-Terminal Amphipathic Helix in Hepatitis C Virus (HCV) NS4B Mediates Membrane Association, Correct Localization of Replication Complex Proteins, and HCV RNA Replication J. Virol., October 15, 2004; 78(20): 11393 - 11400. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Rescher and V. Gerke Annexins - unique membrane binding proteins with diverse functions J. Cell Sci., June 1, 2004; 117(13): 2631 - 2639. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Schuck, A. Manninen, M. Honsho, J. Fullekrug, and K. Simons Generation of single and double knockdowns in polarized epithelial cells by retrovirus-mediated RNA interference PNAS, April 6, 2004; 101(14): 4912 - 4917. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Delacour, V. Gouyer, E. Leteurtre, T. Ait-Slimane, H. Drobecq, C. Lenoir, O. Moreau-Hannedouche, G. Trugnan, and G. Huet 1-Benzyl-2-acetamido-2-deoxy-{alpha}-D-galactopyranoside Blocks the Apical Biosynthetic Pathway in Polarized HT-29 Cells J. Biol. Chem., September 26, 2003; 278(39): 37799 - 37809. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. L. TUMA and A. L. HUBBARD Transcytosis: Crossing Cellular Barriers Physiol Rev, July 1, 2003; 83(3): 871 - 932. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Elazar, K. H. Cheong, P. Liu, H. B. Greenberg, C. M. Rice, and J. S. Glenn Amphipathic Helix-Dependent Localization of NS5A Mediates Hepatitis C Virus RNA Replication J. Virol., May 15, 2003; 77(10): 6055 - 6061. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Nunziante, S. Gilch, and H. M. Schatzl Essential Role of the Prion Protein N Terminus in Subcellular Trafficking and Half-life of Cellular Prion Protein J. Biol. Chem., January 31, 2003; 278(6): 3726 - 3734. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-M. Iglesias, R. O. Morgan, N. A. Jenkins, N. G. Copeland, D. J. Gilbert, and M.-P. Fernandez Comparative Genetics and Evolution of Annexin A13 as the Founder Gene of Vertebrate Annexins Mol. Biol. Evol., May 1, 2002; 19(5): 608 - 618. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Gerke and S. E. Moss Annexins: From Structure to Function Physiol Rev, April 1, 2002; 82(2): 331 - 371. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. B. Babiychuk and A. Draeger Annexins in Cell Membrane Dynamics: Ca2+-regulated Association of Lipid Microdomains J. Cell Biol., September 5, 2000; 150(5): 1113 - 1124. [Abstract] [Full Text] [PDF]
|
|
