spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ohsawa, K.
Right arrow Articles by Kohsaka, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ohsawa, K.
Right arrow Articles by Kohsaka, S.

Journal of Cell Science, Vol 113, Issue 17 3073-3084, Copyright © 2000 by Company of Biologists

JOURNAL ARTICLES

Involvement of Iba1 in membrane ruffling and phagocytosis of macrophages/microglia

K Ohsawa, Y Imai, H Kanazawa, Y Sasaki and S Kohsaka
Department of Neurochemistry, National Institute of Neuroscience, Kodaira, Tokyo 187-8502, Japan.

Ionized calcium binding adaptor molecule 1, Iba1, is an EF hand calcium binding protein whose expression is restricted to macrophages/microglia. In this study, Iba1 was shown to colocalize with F-actin in membrane ruffles induced by macrophage colony-stimulating factor and in phagocytic cups formed during zymosan phagocytosis. Expression of mutant Iba1 carrying either N- or C-terminal deletions or carrying a substitution in the calcium binding domain, suppressed the membrane ruffling and the phagocytosis. These results indicate that Iba1 is a key molecule in membrane ruffling and the phagocytosis of macrophages/microglia. Furthermore, Iba1 colocalized with a small GTPase Rac in the membrane ruffles and the phagocytic cups. The Iba1 mutants also suppressed membrane ruffling induced by dominant active Rac1V12, but do not affect microspikes by Cdc42V12 and stress fibers by RhoAV14. These observations suggest that Iba1 is involved in Rac and calcium signaling pathways.


This article has been cited by other articles:


Home page
 IOVSHome page
A. Bosco, D. M. Inman, M. R. Steele, G. Wu, I. Soto, N. Marsh-Armstrong, W. C. Hubbard, D. J. Calkins, P. J. Horner, and M. L. Vetter
Reduced Retina Microglial Activation and Improved Optic Nerve Integrity with Minocycline Treatment in the DBA/2J Mouse Model of Glaucoma
Invest. Ophthalmol. Vis. Sci., April 1, 2008; 49(4): 1437 - 1446.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
E. C. Johnson, L. Jia, W. O. Cepurna, T. A. Doser, and J. C. Morrison
Global Changes in Optic Nerve Head Gene Expression after Exposure to Elevated Intraocular Pressure in a Rat Glaucoma Model
Invest. Ophthalmol. Vis. Sci., July 1, 2007; 48(7): 3161 - 3177.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
Z. Ahmed, G. Shaw, V. P. Sharma, C. Yang, E. McGowan, and D. W. Dickson
Actin-binding Proteins Coronin-1a and IBA-1 Are Effective Microglial Markers for Immunohistochemistry
J. Histochem. Cytochem., July 1, 2007; 55(7): 687 - 700.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
N. Davoust, C. Vuaillat, G. Cavillon, C. Domenget, E. Hatterer, A. Bernard, C. Dumontel, P. Jurdic, C. Malcus, C. Confavreux, et al.
Bone marrow CD34+/B220+ progenitors target the inflamed brain and display in vitro differentiation potential toward microglia
FASEB J, October 1, 2006; 20(12): 2081 - 2092.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
S. Saraswathy, G. Wu, and N. A. Rao
Retinal microglial activation and chemotaxis by docosahexaenoic Acid hydroperoxide.
Invest. Ophthalmol. Vis. Sci., August 1, 2006; 47(8): 3656 - 3663.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
J. Villeneuve, P. Tremblay, and L. Vallieres
Tumor Necrosis Factor Reduces Brain Tumor Growth by Enhancing Macrophage Recruitment and Microcyst Formation
Cancer Res., May 1, 2005; 65(9): 3928 - 3936.
[Abstract] [Full Text] [PDF]

Home page
 J AndrolHome page
H. Iida, H. Yamashita, M. Doiguchi, and T. Kaneko
Molecular Cloning of Rat Spergen-3, a Spermatogenic Cell-Specific Gene-3, Encoding a Novel 75-kDa Protein Bearing EF-Hand Motifs
J Androl, November 1, 2004; 25(6): 885 - 892.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
H. Ellinger-Ziegelbauer, B. Stuart, B. Wahle, W. Bomann, and H.-J. Ahr
Characteristic Expression Profiles Induced by Genotoxic Carcinogens in Rat Liver
Toxicol. Sci., January 1, 2004; 77(1): 19 - 34.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
I. Mori, F. Goshima, Y. Imai, S. Kohsaka, T. Sugiyama, T. Yoshida, T. Yokochi, Y. Nishiyama, and Y. Kimura
Olfactory receptor neurons prevent dissemination of neurovirulent influenza A virus into the brain by undergoing virus-induced apoptosis
J. Gen. Virol., September 1, 2002; 83(9): 2109 - 2116.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
R. Naskar, M. Wissing, and S. Thanos
Detection of Early Neuron Degeneration and Accompanying Microglial Responses in the Retina of a Rat Model of Glaucoma
Invest. Ophthalmol. Vis. Sci., September 1, 2002; 43(9): 2962 - 2968.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
L. L. Jones and M. H. Tuszynski
Spinal Cord Injury Elicits Expression of Keratan Sulfate Proteoglycans by Macrophages, Reactive Microglia, and Oligodendrocyte Progenitors
J. Neurosci., June 1, 2002; 22(11): 4611 - 4624.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Kanazawa, K. Ohsawa, Y. Sasaki, S. Kohsaka, and Y. Imai
Macrophage/Microglia-specific Protein Iba1 Enhances Membrane Ruffling and Rac Activation via Phospholipase C-gamma -dependent Pathway
J. Biol. Chem., May 24, 2002; 277(22): 20026 - 20032.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
M. Doiguchi, H. Yamashita, J. Ichinose, T. Mori, Y. Shibata, and H. Iida
Complementary DNA Cloning and Characterization of Rat spergen-1, a Spermatogenic Cell-Specific Gene-1, Containing a Mitochondria-Targeting Signal
Biol Reprod, May 1, 2002; 66(5): 1462 - 1470.
[Abstract] [Full Text]

Home page
 J. Neurosci.Home page
L. L. Jones, Y. Yamaguchi, W. B. Stallcup, and M. H. Tuszynski
NG2 Is a Major Chondroitin Sulfate Proteoglycan Produced after Spinal Cord Injury and Is Expressed by Macrophages and Oligodendrocyte Progenitors
J. Neurosci., April 1, 2002; 22(7): 2792 - 2803.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
J. Laporte, F. Blondeau, A. Gansmuller, Y. Lutz, J.-L. Vonesch, and J.-L. Mandel
The PtdIns3P phosphatase myotubularin is a cytoplasmic protein that also localizes to Rac1-inducible plasma membrane ruffles
J. Cell Sci., January 8, 2002; 115(15): 3105 - 3117.
[Abstract] [Full Text] [PDF]

Home page
 StrokeHome page
D. Ito, K. Tanaka, S. Suzuki, T. Dembo, and Y. Fukuuchi
Enhanced Expression of Iba1, Ionized Calcium-Binding Adapter Molecule 1, After Transient Focal Cerebral Ischemia In Rat Brain
Stroke, May 1, 2001; 32(5): 1208 - 1215.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
H. Iida, M. Doiguchi, H. Yamashita, S. Sugimachi, J. Ichinose, T. Mori, and Y. Shibata
Spermatid-Specific Expression of Iba1, an Ionized Calcium Binding Adapter Molecule-1, in Rat Testis
Biol Reprod, April 1, 2001; 64(4): 1138 - 1146.
[Abstract] [Full Text]

Home page
 J. Neurosci.Home page
S. Honda, Y. Sasaki, K. Ohsawa, Y. Imai, Y. Nakamura, K. Inoue, and S. Kohsaka
Extracellular ATP or ADP Induce Chemotaxis of Cultured Microglia through Gi/o-Coupled P2Y Receptors
J. Neurosci., March 15, 2001; 21(6): 1975 - 1982.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2000