|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 113, Issue 18 3217-3226, Copyright © 2000 by Company of Biologists
JOURNAL ARTICLES |
E Kaszas and WZ Cande
341 LSA, Department of Molecular and Cell Biology, University of California Berkeley, CA 94720, USA. etienne.kaszas@nabri.novartis.com
Meiotic chromosome condensation is a unique process, characterized by dramatic changes in chromosome morphology that are required for the correct progression of pairing, synapsis, recombination and segregation of sister chromatids. We used an antibody that recognizes a ser 10 phosphoepitope on histone H3 to monitor H3 phosphorylation during meiosis in maize meiocytes. H3 phosphorylation has been reported to be an excellent marker for chromosome condensation during mitotic prophase in animal cells. In this study, we find that on maize mitotic chromosomes only pericentromeric regions are stained; there is little staining on the arms. During meiosis, chromosome condensation from leptotene through diplotene occurs in the absence of H3 phosphorylation. Instead, the changes in H3 phosphorylation at different stages of meiosis correlate with the differences in requirements for sister chromatid cohesion at different stages. Just before nuclear envelope breakdown, histone H3 phosphorylation is seen first in the pericentromeric regions and then extends through the arms at metaphase I; at metaphase II only the pericentromeric regions are stained. In afd1 (absence of first division), a mutant that is defective in many aspects of meiosis including sister chromatid cohesion and has equational separation at metaphase I, staining is restricted to the pericentromeric regions during metaphase I and anaphase I; there is no staining at metaphase II or anaphase II. We conclude that changes in the level of phosphorylation of ser10 in H3 correspond to changes in the cohesion of sister chromatids rather than the extent of chromosome condensation at different stages of meiosis.
This article has been cited by other articles:
|
|
 |
|
  J. E. Swain, J. Ding, J. Wu, and G. D. Smith Regulation of spindle and chromatin dynamics during early and late stages of oocyte maturation by aurora kinases Mol. Hum. Reprod., May 1, 2008; 14(5): 291 - 299. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Zaccarini, F. P. Cordelieres, P. Martin, and S. Saule PAX6 P46 Binds Chromosomes in the Pericentromeric Region and Induces a Mitosis Defect When Overexpressed Invest. Ophthalmol. Vis. Sci., December 1, 2007; 48(12): 5408 - 5419. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Ronceret, C. G. Bozza, and W. P. Pawlowski Naughty Behavior of Maize Minichromosomes in Meiosis PLANT CELL, December 1, 2007; 19(12): 3835 - 3837. [Full Text] [PDF]
|
|
|
|
|
|
F. Han, Z. Gao, W. Yu, and J. A. Birchler Minichromosome Analysis of Chromosome Pairing, Disjunction, and Sister Chromatid Cohesion in Maize PLANT CELL, December 1, 2007; 19(12): 3853 - 3863. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Ito Role of Histone Modification in Chromatin Dynamics J. Biochem., May 1, 2007; 141(5): 609 - 614. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. E. Swain, J. Ding, D. L. Brautigan, E. Villa-Moruzzi, and G. D. Smith Proper Chromatin Condensation and Maintenance of Histone H3 Phosphorylation During Mouse Oocyte Meiosis Requires Protein Phosphatase Activity Biol Reprod, April 1, 2007; 76(4): 628 - 638. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. N. Golubovskaya, O. Hamant, L. Timofejeva, C.-J. R. Wang, D. Braun, R. Meeley, and W. Z. Cande Alleles of afd1 dissect REC8 functions during meiotic prophase I J. Cell Sci., August 15, 2006; 119(16): 3306 - 3315. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Jelinkova and M. Kubelka Neither Aurora B Activity nor Histone H3 Phosphorylation Is Essential for Chromosome Condensation During Meiotic Maturation of Porcine Oocytes Biol Reprod, May 1, 2006; 74(5): 905 - 912. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Han, J. C. Lamb, and J. A. Birchler High frequency of centromere inactivation resulting in stable dicentric chromosomes of maize PNAS, February 28, 2006; 103(9): 3238 - 3243. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Demidov, D. Van Damme, D. Geelen, F. R. Blattner, and A. Houben Identification and Dynamics of Two Classes of Aurora-Like Kinases in Arabidopsis and Other Plants PLANT CELL, March 1, 2005; 17(3): 836 - 848. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Zhang, X. Li, J. B. Marshall, C. X. Zhong, and R. K. Dawe Phosphoserines on Maize CENTROMERIC HISTONE H3 and Histone H3 Demarcate the Centromere and Pericentromere during Chromosome Segregation PLANT CELL, February 1, 2005; 17(2): 572 - 583. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Shibata and M. Murata Differential localization of the centromere-specific proteins in the major centromeric satellite of Arabidopsis thaliana J. Cell Sci., June 15, 2004; 117(14): 2963 - 2970. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-T. Bui, E. Yamaoka, and T. Miyano Involvement of Histone H3 (Ser10) Phosphorylation in Chromosome Condensation Without Cdc2 Kinase and Mitogen-Activated Protein Kinase Activation in Pig Oocytes Biol Reprod, June 1, 2004; 70(6): 1843 - 1851. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Grimanelli, M. Garcia, E. Kaszas, E. Perotti, and O. Leblanc Heterochronic Expression of Sexual Reproductive Programs During Apomictic Development in Tripsacum Genetics, November 1, 2003; 165(3): 1521 - 1531. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Prigent and S. Dimitrov Phosphorylation of serine 10 in histone H3, what for? J. Cell Sci., September 15, 2003; 116(18): 3677 - 3685. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J W. Dieker, J van der Vlag, and J H. Berden Triggers for anti-chromatin autoantibody production in SLE Lupus, December 1, 2002; 11(12): 856 - 864. [Abstract] [PDF]
|
|
|
|
 |
|
 A. Losada, M. Hirano, and T. Hirano Cohesin release is required for sister chromatid resolution, but not for condensin-mediated compaction, at the onset of mitosis Genes & Dev., December 1, 2002; 16(23): 3004 - 3016. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. C. Reyes, L. Hennig, and W. Gruissem Chromatin-Remodeling and Memory Factors. New Regulators of Plant Development Plant Physiology, November 1, 2002; 130(3): 1090 - 1101. [Full Text] [PDF]
|
|
|
|
 |
|
 M. E. Murnion, R. R. Adams, D. M. Callister, C. D. Allis, W. C. Earnshaw, and J. R. Swedlow Chromatin-associated Protein Phosphatase 1 Regulates Aurora-B and Histone H3 Phosphorylation J. Biol. Chem., July 6, 2001; 276(28): 26656 - 26665. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Scrittori, F. Hans, D. Angelov, M. Charra, C. Prigent, and S. Dimitrov pEg2 Aurora-A Kinase, Histone H3 Phosphorylation, and Chromosome Assembly in Xenopus Egg Extract J. Biol. Chem., August 3, 2001; 276(32): 30002 - 30010. [Abstract] [Full Text] [PDF]
|
|
