Journal of Cell Science, Vol 113, Issue 19 3399-3408, Copyright © 2000 by Company of Biologists

JOURNAL ARTICLES

The budding yeast Dbf2 protein kinase localises to the centrosome and moves to the bud neck in late mitosis

LM Frenz, SE Lee, D Fesquet and LH Johnston
Division of Yeast Genetics, National Institute for Medical Research, Medical Research Council, The Ridgeway, Mill Hill, London NW7 1AA, UK.

Dbf2 is a multifunctional protein kinase in Saccharomyces cerevisiae that functions in transcription, the stress response and as part of a network of genes in exit from mitosis. By analogy with fission yeast it seemed likely that these mitotic exit genes would be involved in cytokinesis. As a preliminary investigation of this we have used Dbf2 tagged with GFP to examine intracellular localisation of the protein in living cells. Dbf2 is found on the centrosomes/spindle pole bodies (SPBs) and also at the bud neck where it forms a double ring. The localisation of Dbf2 is cell cycle regulated. It is on the SPBs for much of the cell cycle and migrates from there to the bud neck in late mitosis, consistent with a role in cytokinesis. Dbf2 partly co-localises with septins at the bud neck. A temperature-sensitive mutant of dbf2 also blocks progression of cytokinesis at 37 degrees C. Following cytokinesis some Dbf2 moves into the nascent bud. Localisation to the bud neck depends upon the septins and also the mitotic exit network proteins Mob1, Cdc5, Cdc14 and Cdc15. The above data are consistent with Dbf2 acting downstream in a pathway controlling cytokinesis.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				M. A. Trammell, N. M. Mahoney, D. A. Agard, and R. D. Vale Mob4 plays a role in spindle focusing in Drosophila S2 cells J. Cell Sci., April 15, 2008; 121(8): 1284 - 1292. [Abstract] [Full Text] [PDF]

				T. Shimizu, L.-L. Ho, and Z.-C. Lai The mob as tumor suppressor Gene Is Essential for Early Development and Regulates Tissue Growth in Drosophila Genetics, February 1, 2008; 178(2): 957 - 965. [Abstract] [Full Text] [PDF]

				M. Iwase, J. Luo, E. Bi, and A. Toh-e Shs1 Plays Separable Roles in Septin Organization and Cytokinesis in Saccharomyces cerevisiae Genetics, September 1, 2007; 177(1): 215 - 229. [Abstract] [Full Text] [PDF]

				N. Yabuta, N. Okada, A. Ito, T. Hosomi, S. Nishihara, Y. Sasayama, A. Fujimori, D. Okuzaki, H. Zhao, M. Ikawa, et al. Lats2 Is an Essential Mitotic Regulator Required for the Coordination of Cell Division J. Biol. Chem., June 29, 2007; 282(26): 19259 - 19271. [Abstract] [Full Text] [PDF]

				H.-O. Park and E. Bi Central Roles of Small GTPases in the Development of Cell Polarity in Yeast and Beyond Microbiol. Mol. Biol. Rev., March 1, 2007; 71(1): 48 - 96. [Abstract] [Full Text] [PDF]

				A. Clemente-Blanco, A. Gonzalez-Novo, F. Machin, D. Caballero-Lima, L. Aragon, M. Sanchez, C. R. V. de Aldana, J. Jimenez, and J. Correa-Bordes The Cdc14p phosphatase affects late cell-cycle events and morphogenesis in Candida albicans J. Cell Sci., March 15, 2006; 119(6): 1130 - 1143. [Abstract] [Full Text] [PDF]

				J. Stoepel, M. A. Ottey, C. Kurischko, P. Hieter, and F. C. Luca The Mitotic Exit Network Mob1p-Dbf2p Kinase Complex Localizes to the Nucleus and Regulates Passenger Protein Localization Mol. Biol. Cell, December 1, 2005; 16(12): 5465 - 5479. [Abstract] [Full Text] [PDF]

				V. Denis and M. S. Cyert Molecular Analysis Reveals Localization of Saccharomyces cerevisiae Protein Kinase C to Sites of Polarized Growth and Pkc1p Targeting to the Nucleus and Mitotic Spindle Eukaryot. Cell, January 1, 2005; 4(1): 36 - 45. [Abstract] [Full Text] [PDF]

				D. D'Amours and A. Amon At the interface between signaling and executing anaphase--Cdc14 and the FEAR network Genes & Dev., November 1, 2004; 18(21): 2581 - 2595. [Abstract] [Full Text] [PDF]

				H. Hwa Lim, F. M. Yeong, and U. Surana Inactivation of Mitotic Kinase Triggers Translocation of MEN Components to Mother-Daughter Neck in Yeast Mol. Biol. Cell, November 1, 2003; 14(11): 4734 - 4743. [Abstract] [Full Text] [PDF]

				A. J. Bardin, M. G. Boselli, and A. Amon Mitotic Exit Regulation through Distinct Domains within the Protein Kinase Cdc15 Mol. Cell. Biol., July 15, 2003; 23(14): 5018 - 5030. [Abstract] [Full Text] [PDF]

				S. Jensen, M. Geymonat, A. L. Johnson, M. Segal, and L. H. Johnston Spatial regulation of the guanine nucleotide exchange factor Lte1 in Saccharomyces cerevisiae J. Cell Sci., March 14, 2003; 115(24): 4977 - 4991. [Abstract] [Full Text] [PDF]

				M.-C. Hou, D. J. Wiley, F. Verde, and D. McCollum Mob2p interacts with the protein kinase Orb6p to promote coordination of cell polarity with cell cycle progression J. Cell Sci., January 1, 2003; 116(1): 125 - 135. [Abstract] [Full Text] [PDF]

				V. J. Cid, J. Jimenez, M. Molina, M. Sanchez, C. Nombela, and J. W. Thorner Orchestrating the cell cycle in yeast: sequential localization of key mitotic regulators at the spindle pole and the bud neck Microbiology, September 1, 2002; 148(9): 2647 - 2659. [Full Text] [PDF]

				D. A. Guertin, S. Trautmann, and D. McCollum Cytokinesis in Eukaryotes Microbiol. Mol. Biol. Rev., June 1, 2002; 66(2): 155 - 178. [Abstract] [Full Text] [PDF]

				S. E. Lee, S. Jensen, L. M. Frenz, A. L. Johnson, D. Fesquet, and L. H. Johnston The Bub2-dependent mitotic pathway in yeast acts every cell cycle and regulates cytokinesis J. Cell Sci., March 8, 2002; 114(12): 2345 - 2354. [Abstract] [Full Text] [PDF]

				J. Bembenek and H. Yu Regulation of the Anaphase-promoting Complex by the Dual Specificity Phosphatase Human Cdc14a J. Biol. Chem., December 14, 2001; 276(51): 48237 - 48242. [Abstract] [Full Text] [PDF]

				F. C. Luca, M. Mody, C. Kurischko, D. M. Roof, T. H. Giddings, and M. Winey Saccharomyces cerevisiae Mob1p Is Required for Cytokinesis and Mitotic Exit Mol. Cell. Biol., October 15, 2001; 21(20): 6972 - 6983. [Abstract] [Full Text] [PDF]

				R. Visintin and A. Amon Regulation of the Mitotic Exit Protein Kinases Cdc15 and Dbf2 Mol. Biol. Cell, October 1, 2001; 12(10): 2961 - 2974. [Abstract] [Full Text] [PDF]

				C. R. Bartholomew, S. H. Woo, Y. S. Chung, C. Jones, and C. F. J. Hardy Cdc5 Interacts with the Wee1 Kinase in Budding Yeast Mol. Cell. Biol., August 1, 2001; 21(15): 4949 - 4959. [Abstract] [Full Text] [PDF]

				A. S. Mah, J. Jang, and R. J. Deshaies Protein kinase Cdc15 activates the Dbf2-Mob1 kinase complex PNAS, June 7, 2001; (2001) 141098998. [Abstract] [Full Text] [PDF]

				K. Asakawa, S. Yoshida, F. Otake, and A. Toh-e A Novel Functional Domain of Cdc15 Kinase Is Required for Its Interaction With Tem1 GTPase in Saccharomyces cerevisiae Genetics, April 1, 2001; 157(4): 1437 - 1450. [Abstract] [Full Text]

				J Lippincott, K. Shannon, W Shou, R. Deshaies, and R Li The Tem1 small GTPase controls actomyosin and septin dynamics during cytokinesis J. Cell Sci., January 4, 2001; 114(7): 1379 - 1386. [Abstract] [PDF]

				C. S. Moreno, W. S. Lane, and D. C. Pallas A Mammalian Homolog of Yeast MOB1 Is Both a Member and a Putative Substrate of Striatin Family-Protein Phosphatase 2A Complexes J. Biol. Chem., June 22, 2001; 276(26): 24253 - 24260. [Abstract] [Full Text] [PDF]

				A. S. Mah, J. Jang, and R. J. Deshaies Protein kinase Cdc15 activates the Dbf2-Mob1 kinase complex PNAS, June 19, 2001; 98(13): 7325 - 7330. [Abstract] [Full Text] [PDF]