spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Murray, J.
Right arrow Articles by Kellie, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Murray, J.
Right arrow Articles by Kellie, S.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Journal of Cell Science, Vol 113, Issue 2 337-348, Copyright © 2000 by Company of Biologists

JOURNAL ARTICLES

Phosphatidylinositol-3' kinase-dependent vesicle formation in macrophages in response to macrophage colony stimulating factor

J Murray, L Wilson and S Kellie
Yamanouchi Research Institute, Littlemore Park, Oxford OX4 4XS, UK.

Treatment of the BAC1.2F5 macrophage cell line with Macrophage Colony Stimulating Factor (M-CSF) resulted in a rapid induction of vesiculation that was reminiscent of macropinocytosis. Time-lapse micrography showed that these vesicles initiated as small vesicles at the cell periphery, but grew in size and migrated with time to a perinuclear localisation after growth factor stimulation. Immunofluorescence showed that the M-CSF receptor (c-fms) associated with the small vesicles and also the larger phase-bright vesicles. Treatment with two distinct inhibitors showed that the rapid initiation of vesicle formation was not dependent on phosphatidylinositol-3' (PI-3) kinase activity; however, the subsequent maintenance, maturation and translocation of the large, phase-bright, c-fms-containing vesicles was dependent on PI-3 kinase activity. The inhibitors could also reverse the further maturation of preformed vesicles. The inhibition of vesicle trafficking and maturation correlated with ablation of M-CSF-induced PI-3 kinase activity associated with p110(alpha). These data demonstrate a role for PI-3 kinase in vesicle trafficking and maintenance. PI-3 kinase activity was also necessary for the macropinocytotic response in macrophages, a process that is essential for efficient antigen processing and presentation in macrophage-like cells.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
A. Achuthan, P. Masendycz, J. A. Lopez, T. Nguyen, D. E. James, M. J. Sweet, J. A. Hamilton, and G. M. Scholz
Regulation of the Endosomal SNARE Protein Syntaxin 7 by Colony-Stimulating Factor 1 in Macrophages
Mol. Cell. Biol., October 15, 2008; 28(20): 6149 - 6159.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
Y. Li and I. Tabas
The inflammatory cytokine response of cholesterol-enriched macrophages is dampened by stimulated pinocytosis
J. Leukoc. Biol., February 1, 2007; 81(2): 483 - 491.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
W. Xu, A. Roos, N. Schlagwein, A. M. Woltman, M. R. Daha, and C. van Kooten
IL-10-producing macrophages preferentially clear early apoptotic cells
Blood, June 15, 2006; 107(12): 4930 - 4937.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
L. Shen and J. R. Turner
Actin Depolymerization Disrupts Tight Junctions via Caveolae-mediated Endocytosis
Mol. Biol. Cell, September 1, 2005; 16(9): 3919 - 3936.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. N. Abell, A. M. DeCathelineau, S. A. Weed, D. R. Ambruso, D. W. Riches, and G. L. Johnson
Rac2D57N, a dominant inhibitory Rac2 mutant that inhibits p38 kinase signaling and prevents surface ruffling in bone-marrow-derived macrophages
J. Cell Sci., January 15, 2004; 117(2): 243 - 255.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
M. Amyere, B. Payrastre, U. Krause, P. V. D. Smissen, A. Veithen, and P. J. Courtoy
Constitutive Macropinocytosis in Oncogene-transformed Fibroblasts Depends on Sequential Permanent Activation of Phosphoinositide 3-Kinase and Phospholipase C
Mol. Biol. Cell, October 1, 2000; 11(10): 3453 - 3467.
[Abstract] [Full Text]


© The Company of Biologists Ltd 2000