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Journal of Cell Science, Vol 113, Issue 22 3939-3946, Copyright © 2000 by Company of Biologists
JOURNAL ARTICLES |
V Prahlad, BT Helfand, GM Langford, RD Vale and RD Goldman
Northwestern University Medical School, Department of Cell and Molecular Biology, Chicago, IL 60611, USA.
Using squid axoplasm as a model system, we have visualized the fast transport of non-filamentous neurofilament protein particles along axonal microtubules. This transport occurs at speeds of 0.5-1.0 microm/second and the majority of neurofilament particles stain with kinesin antibody. These observations demonstrate, for the first time, that fast (0.5-1.0 microm/second) transport of neurofilament proteins occurs along microtubules. In addition, our studies suggest that neurofilament protein can be transported as non-membrane bound, nonfilamentous subunits along axons, and that the transport is kinesin-dependent. Microtubule-based fast transport might therefore provide a mechanism for the distribution and turnover of neurofilament, and perhaps other cytoskeletal proteins, throughout neurons.
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