|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 113, Issue 22 4025-4033, Copyright © 2000 by Company of Biologists
JOURNAL ARTICLES |
I Suriapranata, UD Epple, D Bernreuther, M Bredschneider, K Sovarasteanu and M Thumm
Institut fuer Biochemie, Universitaet Stuttgart, Pfaffenwaldring 55, 70569 Stuttgart.
Autophagy is a degradative transport pathway that delivers cytosolic proteins to the lysosome (vacuole). Cytosolic proteins appear inside the vacuole enclosed in autophagic vesicles. These autophagic vesicles are broken down in the vacuole together with their cytosolic content. The breakdown of vesicular transport intermediates is a unique feature of autophagy. We here identify Aut4p, a component essential for the disintegration of autophagic vesicles, inside the vacuole of S. cerevisiae cells. Aut4p is a putative integral membrane protein with limited homologies to permeases. Chromosomal deletion of AUT4 has no obvious influence on growth, vacuolar acidification and the activities of vacuolar proteinases. Like proteinase B-deficient cells, aut4-deleted cells show a partial reduction in total protein breakdown during nitrogen starvation. A biologically active fusion protein of Aut4p and the green fluorescent protein is visualized at the vacuolar membrane and in punctate structures attached to the vacuole.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  N. Mizushima Autophagy: process and function Genes & Dev., November 15, 2007; 21(22): 2861 - 2873. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Yang, J. Huang, J. Geng, U. Nair, and D. J. Klionsky Atg22 Recycles Amino Acids to Link the Degradative and Recycling Functions of Autophagy Mol. Biol. Cell, December 1, 2006; 17(12): 5094 - 5104. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Cebollero and R. Gonzalez Induction of Autophagy by Second-Fermentation Yeasts during Elaboration of Sparkling Wines. Appl. Envir. Microbiol., June 1, 2006; 72(6): 4121 - 4127. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. J. Klionsky The molecular machinery of autophagy: unanswered questions J. Cell Sci., January 1, 2005; 118(1): 7 - 18. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. B. Kunz, H. Schwarz, and A. Mayer Determination of Four Sequential Stages during Microautophagy in Vitro J. Biol. Chem., March 12, 2004; 279(11): 9987 - 9996. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Delhaize, T. Kataoka, D. M. Hebb, R. G. White, and P. R. Ryan Genes Encoding Proteins of the Cation Diffusion Facilitator Family That Confer Manganese Tolerance PLANT CELL, May 1, 2003; 15(5): 1131 - 1142. [Abstract] [Full Text]
|
|
|
|
|
|
U. D. Epple, E.-L. Eskelinen, and M. Thumm Intravacuolar Membrane Lysis in Saccharomyces cerevisiae. DOES VACUOLAR TARGETING OF Cvt17/Aut5p AFFECT ITS FUNCTION? J. Biol. Chem., February 28, 2003; 278(10): 7810 - 7821. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Reggiori and D. J. Klionsky Autophagy in the Eukaryotic Cell Eukaryot. Cell, February 1, 2002; 1(1): 11 - 21. [Full Text] [PDF]
|
|
|
|
 |
|
 U. D. Epple, I. Suriapranata, E.-L. Eskelinen, and M. Thumm Aut5/Cvt17p, a Putative Lipase Essential for Disintegration of Autophagic Bodies inside the Vacuole J. Bacteriol., October 15, 2001; 183(20): 5942 - 5955. [Abstract] [Full Text] [PDF]
|
|
