|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 113, Issue 23 4263-4273, Copyright © 2000 by Company of Biologists
JOURNAL ARTICLES |
RP Herzig, U Andersson and RC Scarpulla
Department of Cell and Molecular Biology, Northwestern University Medical School, Chicago, Illinois 60611, USA.
Nuclear respiratory factor-1 is a transcriptional activator that has been implicated in the nuclear control of respiratory chain expression. Yeast two-hybrid screens were performed to identify proteins that physically interact with nuclear respiratory factor-1. Saturation screening of both mouse embryo and mouse testis libraries yielded 14 independent clones, all of which represented two different isoforms of dynein light chain. In addition to using the two-hybrid method, the specificity of the nuclear respiratory factor-1/dynein light chain interaction was established by chemical crosslinking of the purified native proteins and by co-immunoprecipitation of nuclear respiratory factor-1 and dynein light chain from mammalian cells. Both two-hybrid and chemical crosslinking assays demonstrated that binding of dynein light chain required the first 26 amino acids of nuclear respiratory factor-1. Although dynein light chain is associated with dynein, a cytoplasmic motor molecule, immunolocalizations showed substantial nuclear staining using several different anti-dynein light chain antibodies. Moreover, fluorescence overlays of confocal images established that nuclear respiratory factor-1 and dynein light chain displayed a very similar nuclear staining pattern. The significance of the nuclear respiratory factor-1/dynein light chain interaction was investigated further by determining whether a similar interaction was conserved between dynein light chain and the erect wing gene product of Drosophila, a protein related to nuclear respiratory factor-1 through its DNA binding domain. Here, we establish that the erect wing gene product can bind and trans-activate transcription through authentic nuclear respiratory factor-1 binding sites. Moreover, the erect wing gene product, like nuclear respiratory factor-1, interacted specifically with dynein light chain both in vitro and in transfected cells. Thus, the interaction with dynein light chain is conserved between transcription factors that are structurally and functionally similar between humans and Drosophila.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Dorsett and T. Schedl A Role for Dynein in the Inhibition of Germ Cell Proliferative Fate Mol. Cell. Biol., November 15, 2009; 29(22): 6128 - 6139. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. B. Hossain, P. Ji, R. Anish, R. H. Jacobson, and S. Takada Poly(ADP-ribose) Polymerase 1 Interacts with Nuclear Respiratory Factor 1 (NRF-1) and Plays a Role in NRF-1 Transcriptional Regulation J. Biol. Chem., March 27, 2009; 284(13): 8621 - 8632. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. C. Williams, P. L. Roulhac, A. G. Roy, R. B. Vallee, M. C. Fitzgerald, and W. A. Hendrickson Structural and thermodynamic characterization of a cytoplasmic dynein light chain intermediate chain complex PNAS, June 12, 2007; 104(24): 10028 - 10033. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A.-D. Lajoix, S. Badiou, S. Peraldi-Roux, T. Chardes, S. Dietz, C. Aknin, F. Tribillac, P. Petit, and R. Gross Protein Inhibitor of Neuronal Nitric Oxide Synthase (PIN) Is a New Regulator of Glucose-Induced Insulin Secretion Diabetes, December 1, 2006; 55(12): 3279 - 3288. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. P. Duckles, D. N. Krause, C. Stirone, and V. Procaccio Estrogen and mitochondria: a new paradigm for vascular protection? Mol. Interv., February 1, 2006; 6(1): 26 - 35. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T.-Y. Yeh, J.-Z. Chuang, and C.-H. Sung Dynein light chain rp3 acts as a nuclear matrix-associated transcriptional modulator in a dynein-independent pathway J. Cell Sci., August 1, 2005; 118(15): 3431 - 3443. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Emi, T. Kinoshita, K. Sakamoto, Y. Mineyuki, and K.-i. Shimazaki Isolation of a Protein Interacting with Vfphot1a in Guard Cells of Vicia faba Plant Physiology, July 1, 2005; 138(3): 1615 - 1626. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Gleyzer, K. Vercauteren, and R. C. Scarpulla Control of Mitochondrial Transcription Specificity Factors (TFB1M and TFB2M) by Nuclear Respiratory Factors (NRF-1 and NRF-2) and PGC-1 Family Coactivators Mol. Cell. Biol., February 15, 2005; 25(4): 1354 - 1366. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Ghosh-Roy, M. Kulkarni, V. Kumar, S. Shirolikar, and K. Ray Cytoplasmic Dynein-Dynactin Complex Is Required for Spermatid Growth but Not Axoneme Assembly in Drosophila Mol. Biol. Cell, May 1, 2004; 15(5): 2470 - 2483. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. J. Kaiser, K. Tavassoli, G.-J. Van den Bemd, G. T.G. Chang, B. Horsthemke, T. Moroy, and H.-J. Ludecke Nuclear interaction of the dynein light chain LC8a with the TRPS1 transcription factor suppresses the transcriptional repression activity of TRPS1 Hum. Mol. Genet., June 1, 2003; 12(11): 1349 - 1358. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. C. Fuhrmann, S. Kins, P. Rostaing, O. El Far, J. Kirsch, M. Sheng, A. Triller, H. Betz, and M. Kneussel Gephyrin Interacts with Dynein Light Chains 1 and 2, Components of Motor Protein Complexes J. Neurosci., July 1, 2002; 22(13): 5393 - 5402. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. K. Fazio, T. A. Bolger, and G. Gill Conserved Regions of the Drosophila Erect Wing Protein Contribute Both Positively and Negatively to Transcriptional Activity J. Biol. Chem., May 25, 2001; 276(22): 18710 - 18716. [Abstract] [Full Text] [PDF]
|
|
