spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Borgne, A.
Right arrow Articles by Nurse, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Borgne, A.
Right arrow Articles by Nurse, P.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Journal of Cell Science, Vol 113, Issue 23 4341-4350, Copyright © 2000 by Company of Biologists

JOURNAL ARTICLES

The Spd1p S phase inhibitor can activate the DNA replication checkpoint pathway in fission yeast

A Borgne and P Nurse
Cell Cycle Laboratory, Imperial Cancer Research Found, Lincoln's Inn Fields, London, WC2A 3PX, UK. A.Borgne@icrf.icnet.uk

Spd1p (for S phase delayed) is a cell cycle inhibitor in Schizosaccharomyces pombe. Spd1p overexpression blocks the onset of both S phase and mitosis. In this study, we have investigated the mechanisms by which Spd1p overexpression blocks cell cycle progression, focussing on the block over mitotic onset. High levels of Spd1p lead to an increase in Y15 phosphorylation of Cdc2p and we show that the block over G(2) requires the Wee1p kinase and is dependent on the rad and chk1/cds1 checkpoint genes. We propose that high levels of Spd1p in G(2) cells activate the DNA replication checkpoint control, which leads to a Wee1p-dependent increase of Cdc2p Y15 phosphorylation blocking onset of mitosis. The Spd1p block at S phase onset may act by interfering directly with DNA replication, and also activates the G(2 )rad/hus checkpoint pathway to block mitosis.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 GeneticsHome page
X. An, Z. Zhang, K. Yang, and M. Huang
Cotransport of the Heterodimeric Small Subunit of the Saccharomyces cerevisiae Ribonucleotide Reductase Between the Nucleus and the Cytoplasm
Genetics, May 1, 2006; 173(1): 63 - 73.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Z. Zhang, X. An, K. Yang, D. L. Perlstein, L. Hicks, N. Kelleher, J. Stubbe, and M. Huang
Nuclear localization of the Saccharomyces cerevisiae ribonucleotide reductase small subunit requires a karyopherin and a WD40 repeat protein
PNAS, January 31, 2006; 103(5): 1422 - 1427.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. Hakansson, L. Dahl, O. Chilkova, V. Domkin, and L. Thelander
The Schizosaccharomyces pombe Replication Inhibitor Spd1 Regulates Ribonucleotide Reductase Activity and dNTPs by Binding to the Large Cdc22 Subunit
J. Biol. Chem., January 20, 2006; 281(3): 1778 - 1783.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Bondar, A. Ponomarev, and P. Raychaudhuri
Ddb1 Is Required for the Proteolysis of the Schizosaccharomyces pombe Replication Inhibitor Spd1 during S Phase and after DNA Damage
J. Biol. Chem., March 12, 2004; 279(11): 9937 - 9943.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
C. Liu, K. A. Powell, K. Mundt, L. Wu, A. M. Carr, and T. Caspari
Cop9/signalosome subunits and Pcu4 regulate ribonucleotide reductase by both checkpoint-dependent and -independent mechanisms
Genes & Dev., May 1, 2003; 17(9): 1130 - 1140.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
M. Synnes, E. A. Nilssen, E. Boye, and B. Grallert
A novel chk1-dependent G1/M checkpoint in fission yeast
J. Cell Sci., September 15, 2002; 115(18): 3609 - 3618.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
A. Borgne, H. Murakami, J. Ayte, and P. Nurse
The G1/S Cyclin Cig2p during Meiosis in Fission Yeast
Mol. Biol. Cell, June 1, 2002; 13(6): 2080 - 2090.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2000