|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 113, Issue 24 4413-4420, Copyright © 2000 by Company of Biologists
JOURNAL ARTICLES |
HC Lee and R Aarhus
Department of Pharmacology, University of Minnesota, Minneapolis, MN 55455, USA. leehc@tc.umn.edu
Cells possess multiple Ca(2+) stores and their selective mobilization provides the spatial-temporal Ca(2+) signals crucial in regulating diverse cellular functions. Except for the inositol trisphosphate (IP(3))-sensitive Ca(2+) stores, the identities and the mechanisms of how these internal stores are mobilized are largely unknown. In this study, we describe two Ca(2+) stores, one of which is regulated by cyclic ADP-ribose (cADPR) and the other by nicotinic acid adenine dinucleotide phosphate (NAADP). We took advantage of the large size of the sea urchin egg and stratified its organelles by centrifugation. Using photolysis to produce either uniform or localized increases of cADPR and NAADP from their respective caged analogs, the two separate stores could be visually identified by Ca(2+) imaging and shown to be segregated to the opposite poles of the eggs. The cADPR-pole also contained the IP(3)-sensitive Ca(2+) stores, the egg nucleus and the endoplasmic reticulum (ER); the latter was visualized using Bodipy-thapsigargin. On the other hand, the mitochondria, as visualized by rhodamine 123, were segregated to the opposite pole together with the NAADP-sensitive calcium stores. Fertilization of the stratified eggs elicited a Ca(2+) wave starting at the cADPR-pole and propagating toward the NAADP-pole. These results provide the first direct and visual evidence that the NAADP-sensitive Ca(2+) stores are novel and distinct from the ER. During fertilization, communicating signals appear to be transmitted from the ER to NAADP-sensitive Ca(2+) stores, leading to their activation.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  W. Dammermann, B. Zhang, M. Nebel, C. Cordiglieri, F. Odoardi, T. Kirchberger, N. Kawakami, J. Dowden, F. Schmid, K. Dornmair, et al. NAADP-mediated Ca2+ signaling via type 1 ryanodine receptor in T cells revealed by a synthetic NAADP antagonist PNAS, June 30, 2009; 106(26): 10678 - 10683. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. H. Guse and H. C. Lee NAADP: A Universal Ca2+ Trigger Sci. Signal., November 4, 2008; 1(44): re10 - re10. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Whitaker Calcium signalling in early embryos Phil Trans R Soc B, April 12, 2008; 363(1495): 1401 - 1418. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Steen, T. Kirchberger, and A. H. Guse NAADP Mobilizes Calcium from the Endoplasmic Reticular Ca2+ Store in T-lymphocytes J. Biol. Chem., June 29, 2007; 282(26): 18864 - 18871. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Zhang, G. Zhang, A. Y. Zhang, M. J. Koeberl, E. Wallander, and P.-L. Li Production of NAADP and its role in Ca2+ mobilization associated with lysosomes in coronary arterial myocytes Am J Physiol Heart Circ Physiol, July 1, 2006; 291(1): H274 - H282. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Whitaker Calcium at Fertilization and in Early Development Physiol Rev, January 1, 2006; 86(1): 25 - 88. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. C. Lee Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP)-mediated Calcium Signaling J. Biol. Chem., October 7, 2005; 280(40): 33693 - 33696. [Full Text] [PDF]
|
|
|
|
|
|
W. Dammermann and A. H. Guse Functional Ryanodine Receptor Expression Is Required for NAADP-mediated Local Ca2+ Signaling in T-lymphocytes J. Biol. Chem., June 3, 2005; 280(22): 21394 - 21399. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Galione and O. H. Petersen The NAADP Receptor: New Receptors or New Regulation? Mol. Interv., April 1, 2005; 5(2): 73 - 79. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. P. Kinnear, F.-X. Boittin, J. M. Thomas, A. Galione, and A. M. Evans Lysosome-Sarcoplasmic Reticulum Junctions: A TRIGGER ZONE FOR CALCIUM SIGNALING BY NICOTINIC ACID ADENINE DINUCLEOTIDE PHOSPHATE AND ENDOTHELIN-1 J. Biol. Chem., December 24, 2004; 279(52): 54319 - 54326. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. C. Linden, E. R. Resnik, K. J. Hendrickson, J. M. Herron, T. J. O'Connor, and D. N. Cornfield Chronic intrauterine pulmonary hypertension compromises fetal pulmonary artery smooth muscle cell O2 sensing Am J Physiol Lung Cell Mol Physiol, December 1, 2003; 285(6): L1354 - L1361. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F.-X. Boittin, A. Galione, and A. M. Evans Nicotinic Acid Adenine Dinucleotide Phosphate Mediates Ca2+ Signals and Contraction in Arterial Smooth Muscle via a Two-Pool Mechanism Circ. Res., December 13, 2002; 91(12): 1168 - 1175. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Berridge, G. Dickinson, J. Parrington, A. Galione, and S. Patel Solubilization of Receptors for the Novel Ca2+-mobilizing Messenger, Nicotinic Acid Adenine Dinucleotide Phosphate J. Biol. Chem., November 8, 2002; 277(46): 43717 - 43723. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. A Billington, A. Ho, and A. A Genazzani Nicotinic acid adenine dinucleotide phosphate (NAADP) is present at micromolar concentrations in sea urchin spermatozoa J. Physiol., October 1, 2002; 544(1): 107 - 112. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. N. Chini and F. G. S. De Toledo Nicotinic acid adenine dinucleotide phosphate: a new intracellular second messenger? Am J Physiol Cell Physiol, June 1, 2002; 282(6): C1191 - C1198. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. LIM, K. KYOZUKA, G. GRAGNANIELLO, E. CARAFOLI, and L. SANTELLA NAADP+ initiates the Ca2+ response during fertilization of starfish oocytes FASEB J, October 1, 2001; 15(12): 2257 - 2267. [Abstract] [Full Text] [PDF]
|
|
