|
|
|
|
|
||
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 113, Issue 24 4563-4575, Copyright © 2000 by Company of Biologists
JOURNAL ARTICLES |
Y Ayad-Durieux, P Knechtle, S Goff, F Dietrich and P Philippsen
Department of Molecular Microbiology, Biozentrum, University of Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland.
Filamentous fungi grow by hyphal extension, which is an extreme example of polarized growth. In contrast to yeast species, where polarized growth of the tip of an emerging bud is temporally limited, filamentous fungi exhibit constitutive polarized growth of the hyphal tip. In many fungi, including Ashbya gossypii, polarized growth is reinforced by a process called hyphal maturation. Hyphal maturation refers to the developmental switch from slow-growing hyphae of young mycelium to fast-growing hyphae of mature mycelium. This process is essential for efficient expansion of mycelium. We report for the first time on the identification and characterization of a fungal gene important for hyphal maturation. This novel A. gossypii gene encodes a presumptive PAK (p21-activated kinase)-like kinase. Its closest homolog is the S. cerevisiae Cla4 protein kinase; the A. gossypii protein is therefore called AgCla4p. Agcla4 deletion strains are no longer able to perform the developmental switch from young to mature hyphae, and GFP (green fluorescent protein)-tagged AgCla4p localizes with much higher frequency in mature hyphal tips than in young hyphal tips. Both results support the importance of AgCla4p in hyphal maturation. AgCla4p is also required for septation, indicated by the inability of Agcla4 deletion strains to properly form actin rings and chitin rings. Despite the requirement of AgCla4p for the development of fast-growing hyphae, AgCla4p is not necessary for actin polarization per se, because tips enriched in cortical patches and hyphae with a fully developed network of actin cables can be seen in Agcla4 deletion strains. The possibility that AgCla4p may be involved in regulatory mechanisms that control the dynamics of the actin patches and/or actin cables is discussed.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  C. Lang, S. Grava, T. van den Hoorn, R. Trimble, P. Philippsen, and S. L. Jaspersen Mobility, Microtubule Nucleation and Structure of Microtubule-organizing Centers in Multinucleated Hyphae of Ashbya gossypii Mol. Biol. Cell, January 1, 2010; 21(1): 18 - 28. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Meyer, M. Arentshorst, S. J. Flitter, B. M. Nitsche, M. J. Kwon, C. G. Reynaga-Pena, S. Bartnicki-Garcia, C. A. M. J. J. van den Hondel, and A. F. J. Ram Reconstruction of Signaling Networks Regulating Fungal Morphogenesis by Transcriptomics Eukaryot. Cell, November 1, 2009; 8(11): 1677 - 1691. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. S. DeMay, R. A. Meseroll, P. Occhipinti, and A. S. Gladfelter Regulation of Distinct Septin Rings in a Single Cell by Elm1p and Gin4p Kinases Mol. Biol. Cell, April 15, 2009; 20(8): 2311 - 2326. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Kaufmann and P. Philippsen Of Bars and Rings: Hof1-Dependent Cytokinesis in Multiseptated Hyphae of Ashbya gossypii Mol. Cell. Biol., February 1, 2009; 29(3): 771 - 783. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kohli, V. Galati, K. Boudier, R. W. Roberson, and P. Philippsen Growth-speed-correlated localization of exocyst and polarisome components in growth zones of Ashbya gossypii hyphal tips J. Cell Sci., December 1, 2008; 121(23): 3878 - 3889. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. D. Harris Branching of fungal hyphae: regulation, mechanisms and comparison with other branching systems. Mycologia, November 1, 2008; 100(6): 823 - 832. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. G. Rasmussen and N. L. Glass Localization of RHO-4 Indicates Differential Regulation of Conidial versus Vegetative Septation in the Filamentous Fungus Neurospora crassa Eukaryot. Cell, July 1, 2007; 6(7): 1097 - 1107. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. K. Hungerbuehler, P. Philippsen, and A. S. Gladfelter Limited Functional Redundancy and Oscillation of Cyclins in Multinucleated Ashbya gossypii Fungal Cells Eukaryot. Cell, March 1, 2007; 6(3): 473 - 486. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. S. Gladfelter, N. Sustreanu, A. K. Hungerbuehler, S. Voegeli, V. Galati, and P. Philippsen The Anaphase-Promoting Complex/Cyclosome Is Required for Anaphase Progression in Multinucleated Ashbya gossypii Cells Eukaryot. Cell, February 1, 2007; 6(2): 182 - 197. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Helfer and A. S. Gladfelter AgSwe1p Regulates Mitosis in Response to Morphogenesis and Nutrients in Multinucleated Ashbya gossypii Cells Mol. Biol. Cell, October 1, 2006; 17(10): 4494 - 4512. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-P. Schmitz, A. Kaufmann, M. Kohli, P. P. Laissue, and P. Philippsen From Function to Shape: A Novel Role of a Formin in Morphogenesis of the Fungus Ashbya gossypii Mol. Biol. Cell, January 1, 2006; 17(1): 130 - 145. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. G. Rasmussen and N. L. Glass A Rho-Type GTPase, rho-4, Is Required for Septation in Neurospora crassa Eukaryot. Cell, November 1, 2005; 4(11): 1913 - 1925. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Walther and J. Wendland Apical localization of actin patches and vacuolar dynamics in Ashbya gossypii depend on the WASP homolog Wal1p J. Cell Sci., October 1, 2004; 117(21): 4947 - 4958. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Bauer, P. Knechtle, J. Wendland, H. Helfer, and P. Philippsen A Ras-like GTPase Is Involved in Hyphal Growth Guidance in the Filamentous Fungus Ashbya gossypii Mol. Biol. Cell, October 1, 2004; 15(10): 4622 - 4632. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. B. Nichols, J. A. Fraser, and J. Heitman PAK Kinases Ste20 and Pak1 Govern Cell Polarity at Different Stages of Mating in Cryptococcus neoformans Mol. Biol. Cell, October 1, 2004; 15(10): 4476 - 4489. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. S. Dietrich, S. Voegeli, S. Brachat, A. Lerch, K. Gates, S. Steiner, C. Mohr, R. Pohlmann, P. Luedi, S. Choi, et al. The Ashbya gossypii Genome as a Tool for Mapping the Ancient Saccharomyces cerevisiae Genome Science, April 9, 2004; 304(5668): 304 - 307. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. K. Hicks, C. A. D'Souza, G. M. Cox, and J. Heitman Cyclic AMP-Dependent Protein Kinase Catalytic Subunits Have Divergent Roles in Virulence Factor Production in Two Varieties of the Fungal Pathogen Cryptococcus neoformans Eukaryot. Cell, February 1, 2004; 3(1): 14 - 26. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Knechtle, F. Dietrich, and P. Philippsen Maximal Polar Growth Potential Depends on the Polarisome Component AgSpa2 in the Filamentous Fungus Ashbya gossypii Mol. Biol. Cell, October 1, 2003; 14(10): 4140 - 4154. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Wang, C. B. Nichols, K. B. Lengeler, M. E. Cardenas, G. M. Cox, J. R. Perfect, and J. Heitman Mating-Type-Specific and Nonspecific PAK Kinases Play Shared and Divergent Roles in Cryptococcus neoformans Eukaryot. Cell, April 1, 2002; 1(2): 257 - 272. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C Alberti-Segui, F Dietrich, R Altmann-Johl, D Hoepfner, and P Philippsen Cytoplasmic dynein is required to oppose the force that moves nuclei towards the hyphal tip in the filamentous ascomycete Ashbya gossypii J. Cell Sci., January 3, 2001; 114(5): 975 - 986. [Abstract] [PDF]
|
|
