|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 113, Issue 3 493-506, Copyright © 2000 by Company of Biologists
JOURNAL ARTICLES |
CM Klass, JR Couchman and A Woods
Department of Cell Biology, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
Extracellular matrix (ECM) deposition and organization is maintained by transmembrane signaling and integrins play major roles. We now show that a second transmembrane component, syndecan-2 heparan sulfate proteoglycan, is pivotal in matrix assembly. Chinese Hamster Ovary (CHO) cells were stably transfected with full length (S2) or truncated syndecan-2 lacking the C-terminal 14 amino acids of the cytoplasmic domain (S2deltaS). No differences in the amount of matrix assembly were noted with S2 cells, but those expressing S2deltaS could not assemble laminin or fibronectin into a fibrillar matrix. The loss of matrix formation was not caused by a failure to synthesize or externalize ECM components as determined by metabolic labeling or due to differences in surface expression of alpha5 or beta1 integrin. The matrix assembly defect was at the cell surface, since S2deltaS cells also lost the ability to rearrange laminin or fibronectin substrates into fibrils and to bind exogenous fibronectin. Transfection of activated alphaIIbalphaLdeltabeta3 integrin into alpha(5)-deficient CHO B2 cells resulted in reestablishment of the previously lost fibronectin matrix. However, cotransfection of this cell line with S2deltaS could override the presence of activated integrins. These results suggest a regulatory role for syndecan-2 in matrix assembly, along with previously suggested roles for activated integrins.
This article has been cited by other articles:
|
|
 |
|
  L. L. Galante and J. E. Schwarzbauer Requirements for sulfate transport and the diastrophic dysplasia sulfate transporter in fibronectin matrix assembly J. Cell Biol., December 3, 2007; 179(5): 999 - 1009. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Stepp, Y. Liu, S. Pal-Ghosh, R. A. Jurjus, G. Tadvalkar, A. Sekaran, K. LoSicco, L. Jiang, M. Larsen, L. Li, et al. Reduced migration, altered matrix and enhanced TGFbeta1 signaling are signatures of mouse keratinocytes lacking Sdc1 J. Cell Sci., August 15, 2007; 120(16): 2851 - 2863. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-L. Lin, Y.-T. Lei, C.-J. Hong, and Y.-P. Hsueh Syndecan-2 induces filopodia and dendritic spine formation via the neurofibromin-PKA-Ena/VASP pathway J. Cell Biol., June 21, 2007; 177(5): 829 - 841. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Yoneda, D. Ushakov, H. A.B. Multhaupt, and J. R. Couchman Fibronectin Matrix Assembly Requires Distinct Contributions from Rho Kinases I and -II Mol. Biol. Cell, January 1, 2007; 18(1): 66 - 75. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Zhang, P. Hopwood, C. C. Abrams, A. Downing, F. Murray, R. Talbot, A. Archibald, S. Lowden, and L. K. Dixon Macrophage Transcriptional Responses following In Vitro Infection with a Highly Virulent African Swine Fever Virus Isolate. J. Virol., November 1, 2006; 80(21): 10514 - 10521. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Y. Fears, C. L. Gladson, and A. Woods Syndecan-2 Is Expressed in the Microvasculature of Gliomas and Regulates Angiogenic Processes in Microvascular Endothelial Cells J. Biol. Chem., May 26, 2006; 281(21): 14533 - 14536. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Tkachenko, J. M. Rhodes, and M. Simons Syndecans: New Kids on the Signaling Block Circ. Res., March 18, 2005; 96(5): 488 - 500. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. J. Davies, F. H. Blackhall, J. H. Shanks, G. David, A. T. McGown, R. Swindell, R. J. Slade, P. Martin-Hirsch, J. T. Gallagher, and G. C. Jayson Distribution and Clinical Significance of Heparan Sulfate Proteoglycans in Ovarian Cancer Clin. Cancer Res., August 1, 2004; 10(15): 5178 - 5186. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Chen, D. Lasaitiene, B. G. Gabrielsson, L. M.S. Carlsson, H. Billig, B. Carlsson, N. Marcussen, X.-F. Sun, and P. Friberg Neonatal Losartan Treatment Suppresses Renal Expression of Molecules Involved in Cell-Cell and Cell-Matrix Interactions J. Am. Soc. Nephrol., May 1, 2004; 15(5): 1232 - 1243. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Chen, C. Klass, and A. Woods Syndecan-2 Regulates Transforming Growth Factor-{beta} Signaling J. Biol. Chem., April 16, 2004; 279(16): 15715 - 15718. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Chen, S. Hermanson, and S. C. Ekker Syndecan-2 is essential for angiogenic sprouting during zebrafish development Blood, March 1, 2004; 103(5): 1710 - 1719. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Wierzbicka-Patynowski and J. E. Schwarzbauer The ins and outs of fibronectin matrix assembly J. Cell Sci., August 15, 2003; 116(16): 3269 - 3276. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. K. Thodeti, R. Albrechtsen, M. Grauslund, M. Asmar, C. Larsson, Y. Takada, A. M. Mercurio, J. R. Couchman, and U. M. Wewer ADAM12/Syndecan-4 Signaling Promotes beta 1 Integrin-dependent Cell Spreading through Protein Kinase Calpha and RhoA J. Biol. Chem., March 7, 2003; 278(11): 9576 - 9584. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. K. Greene, S. Tumova, J. R. Couchman, and A. Woods Syndecan-4 Associates with alpha -Actinin J. Biol. Chem., February 21, 2003; 278(9): 7617 - 7623. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Li, D. Harrison, S. Carbonetto, R. Fassler, N. Smyth, D. Edgar, and P. D. Yurchenco Matrix assembly, regulation, and survival functions of laminin and its receptors in embryonic stem cell differentiation J. Cell Biol., June 24, 2002; 157(7): 1279 - 1290. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Santas, J. A. Peterson, J. L. Halbleib, S. E. Craig, M. J. Humphries, and D. M. P. Peters Alternative Splicing of the IIICS Domain in Fibronectin Governs the Role of the Heparin II Domain in Fibrillogenesis and Cell Spreading J. Biol. Chem., April 12, 2002; 277(16): 13650 - 13658. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. V. Tsiper and P. D. Yurchenco Laminin assembles into separate basement membrane and fibrillar matrices in Schwann cells J. Cell Sci., January 3, 2002; 115(5): 1005 - 1015. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Pereira, B. J. Rybarczyk, T. M. Odrljin, D. C. Hocking, J. Sottile, and P. J. Simpson-Haidaris The incorporation of fibrinogen into extracellular matrix is dependent on active assembly of a fibronectin matrix J. Cell Sci., January 2, 2002; 115(3): 609 - 617. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Huang, R. Chiquet-Ehrismann, J. V. Moyano, A. Garcia-Pardo, and G. Orend Interference of Tenascin-C with Syndecan-4 Binding to Fibronectin Blocks Cell Adhesion and Stimulates Tumor Cell Proliferation Cancer Res., December 1, 2001; 61(23): 8586 - 8594. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. P. Moiseeva Adhesion receptors of vascular smooth muscle cells and their functions Cardiovasc Res, December 1, 2001; 52(3): 372 - 386. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Adams, N. Kureishy, and A. L. Taylor A Role for Syndecan-1 in Coupling Fascin Spike Formation by Thrombospondin-1 J. Cell Biol., March 19, 2001; 152(6): 1169 - 1182. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J Sottile, D. Hocking, and K. Langenbach Fibronectin polymerization stimulates cell growth by RGD-dependent and -independent mechanisms J. Cell Sci., January 12, 2000; 113(23): 4287 - 4299. [Abstract] [PDF]
|
|
|
|
|
|
M. Pucci, G. Fibbi, L. Magnelli, and M. Del Rosso Regulation of Urokinase/Urokinase Receptor Interaction by Heparin-like Glycosaminoglycans J. Biol. Chem., February 9, 2001; 276(7): 4756 - 4765. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Tiedemann, B. Batge, P. K. Muller, and D. P. Reinhardt Interactions of Fibrillin-1 with Heparin/Heparan Sulfate, Implications for Microfibrillar Assembly J. Biol. Chem., September 14, 2001; 276(38): 36035 - 36042. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Woods and J. R. Couchman Integrin Modulation by Lateral Association J. Biol. Chem., August 4, 2000; 275(32): 24233 - 24236. [Full Text] [PDF]
|
|
