|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 113, Issue 6 963-973, Copyright © 2000 by Company of Biologists
JOURNAL ARTICLES |
MG Nievers, I Kuikman, D Geerts, IM Leigh and A Sonnenberg
The Netherlands Cancer Institute, Division of Cell Biology, Plesmanlaan 121, The Netherlands.
Hemidesmosomes are adhesion structures that mediate anchorage of epithelial cells to the underlying basement membrane. We have previously shown that the (alpha)6(beta)4 integrin can induce the assembly of these multi-protein structures independent of binding to its ligand laminin-5 (ligand-independent formation of hemidesmosomes). Our results suggested a role for HD1/plectin, which binds to the cytoplasmic domain of the (beta)4 integrin subunit, in controlling the clustering of hemidesmosomal components at the basal side of the cell. Using keratinocytes derived from patients lacking HD1/plectin, we now show that ligand-independent formation of hemidesmosomal clusters indeed requires HD1/plectin, in contrast to the ligand-dependent assembly of hemidesmosomes. No clustering of the (alpha)6(beta)4 integrin, or of the bullous pemphigoid antigens BP180 and BP230, was seen when HD1/plectin-deficient keratinocytes were plated on fibronectin or type IV collagen. In (&bgr;)4-deficient keratinocytes, expression of an interleukin 2 receptor (IL2R) transmembrane chimera containing the (beta)4 cytoplasmic tail with the mutation R1281W, which abrogates HD1/plectin binding, resulted in a diffuse distribution of the chimeric receptor. In contrast, a (beta)4(R1281W) mutant that can associate with (alpha)6 and bind ligand, was found to be directed to the basal surface of the cells, at sites where laminin-5 was deposited. In addition, this mutant induced clustering of BP180 and BP230 at these sites. Together, these results show that the formation of hemidesmosomes requires binding of either ligand or HD1/plectin to the (beta)4 integrin subunit. Intriguingly, we found that IL2R/(beta)4 chimeras become localized in pre-existing hemidesmosomes of HD1/plectin-deficient keratinocytes, and that this localization requires a domain in the (beta)4 cytoplasmic tail that is also required for HD1/plectin binding (residues 1115-1356). Because this part of (beta)4 lacks the BP180 binding site, and since we show in this study that it is unable to interact with the same part on another (beta)4 molecule, we suggest that the chimera becomes incorporated into hemidesmosomes of HD1/plectin-deficient keratinocytes by interacting with an as yet unidentified hemidesmosomal component.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  C. J. Pendegrass, D. Gordon, C. A. Middleton, S. Ng. M. Sun, and G. W. Blunn Sealing the skin barrier around transcutaneous implants: IN VITRO STUDY OF KERATINOCYTE PROLIFERATION AND ADHESION IN RESPONSE TO SURFACE MODIFICATIONS OF TITANIUM ALLOY J Bone Joint Surg Br, January 1, 2008; 90-B(1): 114 - 121. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Wilhelmsen, S. H.M. Litjens, I. Kuikman, C. Margadant, J. van Rheenen, and A. Sonnenberg Serine Phosphorylation of the Integrin beta4 Subunit Is Necessary for Epidermal Growth Factor Receptor induced Hemidesmosome Disruption Mol. Biol. Cell, September 1, 2007; 18(9): 3512 - 3522. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. E. Pullar, B. S. Baier, Y. Kariya, A. J. Russell, B. A.J. Horst, M. P. Marinkovich, and R. R. Isseroff beta4 Integrin and Epidermal Growth Factor Coordinately Regulate Electric Field-mediated Directional Migration via Rac1 Mol. Biol. Cell, November 1, 2006; 17(11): 4925 - 4935. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Tasanen, L. Tunggal, G. Chometon, L. Bruckner-Tuderman, and M. Aumailley Keratinocytes from Patients Lacking Collagen XVII Display a Migratory Phenotype Am. J. Pathol., June 1, 2004; 164(6): 2027 - 2038. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Rabinovitz, L. Tsomo, and A. M. Mercurio Protein Kinase C-{alpha} Phosphorylation of Specific Serines in the Connecting Segment of the {beta}4 Integrin Regulates the Dynamics of Type II Hemidesmosomes Mol. Cell. Biol., May 15, 2004; 24(10): 4351 - 4360. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Koster, S. van Wilpe, I. Kuikman, S.H.M. Litjens, and A. Sonnenberg Role of Binding of Plectin to the Integrin {beta}4 Subunit in the Assembly of Hemidesmosomes Mol. Biol. Cell, March 1, 2004; 15(3): 1211 - 1223. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. A. Rezniczek, C. Abrahamsberg, P. Fuchs, D. Spazierer, and G. Wiche Plectin 5'-transcript diversity: short alternative sequences determine stability of gene products, initiation of translation and subcellular localization of isoforms Hum. Mol. Genet., December 1, 2003; 12(23): 3181 - 3194. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Tsuruta, S. B. Hopkinson, K. D. Lane, M. E. Werner, V. L. Cryns, and J. C. R. Jones Crucial Role of the Specificity-determining Loop of the Integrin {beta}4 Subunit in the Binding of Cells to Laminin-5 and Outside-in Signal Transduction J. Biol. Chem., October 3, 2003; 278(40): 38707 - 38714. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. J. Russell, E. F. Fincher, L. Millman, R. Smith, V. Vela, E. A. Waterman, C. N. Dey, S. Guide, V. M. Weaver, and M. P. Marinkovich {alpha}6{beta}4 integrin regulates keratinocyte chemotaxis through differential GTPase activation and antagonism of {alpha}3{beta}1 integrin J. Cell Sci., September 1, 2003; 116(17): 3543 - 3556. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Koster, D. Geerts, B. Favre, L. Borradori, and A. Sonnenberg Analysis of the interactions between BP180, BP230, plectin and the integrin {alpha}6{beta}4 important for hemidesmosome assembly J. Cell Sci., January 15, 2003; 116(2): 387 - 399. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. W. Geuijen and A. Sonnenberg Dynamics of the alpha 6beta 4 Integrin in Keratinocytes Mol. Biol. Cell, November 1, 2002; 13(11): 3845 - 3858. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. M.Th. Sterk, C. A.W. Geuijen, L. C.J.M. Oomen, J. Calafat, H. Janssen, and A. Sonnenberg The Tetraspan Molecule CD151, a Novel Constituent of Hemidesmosomes, Associates with the Integrin {alpha}6{beta}4 and May Regulate the Spatial Organization of Hemidesmosomes J. Cell Biol., May 15, 2000; 149(4): 969 - 982. [Abstract] [Full Text] [PDF]
|
|
