spacer gif spacer gif spacer gif spacer gif spacer gif
 QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    


This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Muraglia, A.
Right arrow Articles by Quarto, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Muraglia, A.
Right arrow Articles by Quarto, R.

Journal of Cell Science, Vol 113, Issue 7 1161-1166, Copyright © 2000 by Company of Biologists


JOURNAL ARTICLES

Clonal mesenchymal progenitors from human bone marrow differentiate in vitro according to a hierarchical model

A Muraglia, R Cancedda and R Quarto
Centro di Biotecnologie Avanzate, Genova, Italy.

Bone marrow stromal cells can give rise to several mesenchymal lineages. The existence of a common stem/progenitor cell, the mesenchymal stem cell, has been proposed, but which developmental stages follow this mesenchymal multipotent progenitor is not known. Based on experimental evidence, a model of mesenchymal stem cell differentiation has been proposed in which individual lineages branch directly from the same progenitor. We have verified this model by using clonal cultures of bone marrow derived stromal fibroblasts. We have analyzed the ability of 185 non-immortalized human bone marrow stromal cell clones to differentiate into the three main lineages: osteo-, chondro- and adipogenic. All clones but one differentiated into the osteogenic lineage. About one third of the clones differentiated into all three lineages analyzed. Most clones (60-80%) displayed an osteo-chondrogenic potential. We have never observed clones with a differentiation potential limited to the osteo-adipo- or to the chondro-adipogenic phenotype, nor pure chondrogenic and adipogenic clones. How long the differentiation potential of a number of clones was maintained was assessed throughout their life span. Clones progressively lost their adipogenic and chondrogenic differentiation potential at increasing cell doublings. Our data suggest a possible model of predetermined bone marrow stromal cells differentiation where the tripotent cells can be considered as early mesenchymal progenitors that display a sequential loss of lineage potentials, generating osteochondrogenic progenitors which, in turn, give rise to osteogenic precursors.


This article has been cited by other articles:


Home page
haematolHome page
S. Tabera, J. A. Perez-Simon, M. Diez-Campelo, L. I. Sanchez-Abarca, B. Blanco, A. Lopez, A. Benito, E. Ocio, F. M. Sanchez-Guijo, C. Canizo, et al.
The effect of mesenchymal stem cells on the viability, proliferation and differentiation of B-lymphocytes
Haematologica, September 1, 2008; 93(9): 1301 - 1309.
[Abstract] [Full Text] [PDF]


Home page
Stem CellsHome page
D. A. Rider, C. Dombrowski, A. A. Sawyer, G. H. B. Ng, D. Leong, D. W. Hutmacher, V. Nurcombe, and S. M. Cool
Autocrine Fibroblast Growth Factor 2 Increases the Multipotentiality of Human Adipose-Derived Mesenchymal Stem Cells
Stem Cells, June 1, 2008; 26(6): 1598 - 1608.
[Abstract] [Full Text] [PDF]


Home page
Stem CellsHome page
U. Lakshmipathy and R. P. Hart
Concise Review: MicroRNA Expression in Multipotent Mesenchymal Stromal Cells
Stem Cells, February 1, 2008; 26(2): 356 - 363.
[Abstract] [Full Text] [PDF]


Home page
Rheumatology (Oxford)Home page
E. Jones and D. McGonagle
Human bone marrow mesenchymal stem cells in vivo
Rheumatology, February 1, 2008; 47(2): 126 - 131.
[Abstract] [Full Text] [PDF]


Home page
Stem CellsHome page
A. D. Murdoch, L. M. Grady, M. P. Ablett, T. Katopodi, R. S. Meadows, and T. E. Hardingham
Chondrogenic Differentiation of Human Bone Marrow Stem Cells in Transwell Cultures: Generation of Scaffold-Free Cartilage
Stem Cells, November 1, 2007; 25(11): 2786 - 2796.
[Abstract] [Full Text] [PDF]


Home page
Stem CellsHome page
D. G. Phinney and D. J. Prockop
Concise Review: Mesenchymal Stem/Multipotent Stromal Cells: The State of Transdifferentiation and Modes of Tissue Repair Current Views
Stem Cells, November 1, 2007; 25(11): 2896 - 2902.
[Abstract] [Full Text] [PDF]


Home page
Rheumatology (Oxford)Home page
A. English, E. A. Jones, D. Corscadden, K. Henshaw, T. Chapman, P. Emery, and D. McGonagle
A comparative assessment of cartilage and joint fat pad as a potential source of cells for autologous therapy development in knee osteoarthritis
Rheumatology, November 1, 2007; 46(11): 1676 - 1683.
[Abstract] [Full Text] [PDF]


Home page
J. Cell Sci.Home page
F. G. Chen, W. J. Zhang, D. Bi, W. Liu, X. Wei, F. F. Chen, L. Zhu, L. Cui, and Y. Cao
Clonal analysis of nestin vimentin+ multipotent fibroblasts isolated from human dermis
J. Cell Sci., August 15, 2007; 120(16): 2875 - 2883.
[Abstract] [Full Text] [PDF]


Home page
Stem CellsHome page
K. Sudo, M. Kanno, K. Miharada, S. Ogawa, T. Hiroyama, K. Saijo, and Y. Nakamura
Mesenchymal Progenitors Able to Differentiate into Osteogenic, Chondrogenic, and/or Adipogenic Cells In Vitro Are Present in Most Primary Fibroblast-Like Cell Populations
Stem Cells, July 1, 2007; 25(7): 1610 - 1617.
[Abstract] [Full Text] [PDF]


Home page
Stem CellsHome page
S. A. Kuznetsov, M. H. Mankani, A. I. Leet, N. Ziran, S. Gronthos, and P. G. Robey
Circulating Connective Tissue Precursors: Extreme Rarity in Humans and Chondrogenic Potential in Guinea Pigs
Stem Cells, July 1, 2007; 25(7): 1830 - 1839.
[Abstract] [Full Text] [PDF]


Home page
EndocrinologyHome page
O. P. Lazarenko, S. O. Rzonca, W. R. Hogue, F. L. Swain, L. J. Suva, and B. Lecka-Czernik
Rosiglitazone Induces Decreases in Bone Mass and Strength that Are Reminiscent of Aged Bone
Endocrinology, June 1, 2007; 148(6): 2669 - 2680.
[Abstract] [Full Text] [PDF]


Home page
EndocrinologyHome page
V. David, A. Martin, M.-H. Lafage-Proust, L. Malaval, S. Peyroche, D. B. Jones, L. Vico, and A. Guignandon
Mechanical Loading Down-Regulates Peroxisome Proliferator-Activated Receptor {gamma} in Bone Marrow Stromal Cells and Favors Osteoblastogenesis at the Expense of Adipogenesis
Endocrinology, May 1, 2007; 148(5): 2553 - 2562.
[Abstract] [Full Text] [PDF]


Home page
Stem CellsHome page
D. Fang, B.-M. Seo, Y. Liu, W. Sonoyama, T. Yamaza, C. Zhang, S. Wang, and S. Shi
Transplantation of Mesenchymal Stem Cells Is an Optimal Approach for Plastic Surgery
Stem Cells, April 1, 2007; 25(4): 1021 - 1028.
[Abstract] [Full Text] [PDF]


Home page
Stem CellsHome page
T. M. Liu, M. Martina, D. W. Hutmacher, J. H. P. Hui, E. H. Lee, and B. Lim
Identification of Common Pathways Mediating Differentiation of Bone Marrow- and Adipose Tissue-Derived Human Mesenchymal Stem Cells into Three Mesenchymal Lineages
Stem Cells, March 1, 2007; 25(3): 750 - 760.
[Abstract] [Full Text] [PDF]


Home page
Stem CellsHome page
L. Duplomb, M. Dagouassat, P. Jourdon, and D. Heymann
Concise Review: Embryonic Stem Cells: A New Tool to Study Osteoblast and Osteoclast Differentiation
Stem Cells, March 1, 2007; 25(3): 544 - 552.
[Abstract] [Full Text] [PDF]


Home page
BloodHome page
M. Pevsner-Fischer, V. Morad, M. Cohen-Sfady, L. Rousso-Noori, A. Zanin-Zhorov, S. Cohen, I. R. Cohen, and D. Zipori
Toll-like receptors and their ligands control mesenchymal stem cell functions
Blood, February 15, 2007; 109(4): 1422 - 1432.
[Abstract] [Full Text] [PDF]


Home page
BloodHome page
E. J. Gang, D. Bosnakovski, C. A. Figueiredo, J. W. Visser, and R. C. R. Perlingeiro
SSEA-4 identifies mesenchymal stem cells from bone marrow
Blood, February 15, 2007; 109(4): 1743 - 1751.
[Abstract] [Full Text] [PDF]


Home page
BloodHome page
F. Anjos-Afonso and D. Bonnet
Nonhematopoietic/endothelial SSEA-1+ cells define the most primitive progenitors in the adult murine bone marrow mesenchymal compartment
Blood, February 1, 2007; 109(3): 1298 - 1306.
[Abstract] [Full Text] [PDF]


Home page
Stem CellsHome page
P. Tropel, N. Platet, J.-C. Platel, D. Noel, M. Albrieux, A.-L. Benabid, and F. Berger
Functional Neuronal Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells
Stem Cells, December 1, 2006; 24(12): 2868 - 2876.
[Abstract] [Full Text] [PDF]


Home page
Biophys. JHome page
M. A. Dupree, S. R. Pollack, E. M. Levine, and C. T. Laurencin
Fibroblast Growth Factor 2 Induced Proliferation in Osteoblasts and Bone Marrow Stromal Cells: A Whole Cell Model
Biophys. J., October 15, 2006; 91(8): 3097 - 3112.
[Abstract] [Full Text] [PDF]


Home page
J Bone Joint Surg BrHome page
M. Nishimori, M. Deie, A. Kanaya, H. Exham, N. Adachi, and M. Ochi
Repair of chronic osteochondral defects in the rat: A BONE MARROW-STIMULATING PROCEDURE ENHANCED BY CULTURED ALLOGENIC BONE MARROW MESENCHYMAL STROMAL CELLS
J Bone Joint Surg Br, September 1, 2006; 88-B(9): 1236 - 1244.
[Abstract] [Full Text] [PDF]


Home page
J. Immunol.Home page
R. Munoz-Fernandez, F. J. Blanco, C. Frecha, F. Martin, M. Kimatrai, A. C. Abadia-Molina, J. M. Garcia-Pacheco, and E. G. Olivares
Follicular Dendritic Cells Are Related to Bone Marrow Stromal Cell Progenitors and to Myofibroblasts
J. Immunol., July 1, 2006; 177(1): 280 - 289.
[Abstract] [Full Text] [PDF]


Home page
Stem CellsHome page
A. Schmidt, D. Ladage, T. Schinkothe, U. Klausmann, C. Ulrichs, F.-J. Klinz, K. Brixius, S. Arnhold, B. Desai, U. Mehlhorn, et al.
Basic Fibroblast Growth Factor Controls Migration in Human Mesenchymal Stem Cells
Stem Cells, July 1, 2006; 24(7): 1750 - 1758.
[Abstract] [Full Text] [PDF]


Home page
Stem CellsHome page
J. Koerner, D. Nesic, J. D. Romero, W. Brehm, P. Mainil-Varlet, and S. P. Grogan
Equine peripheral blood-derived progenitors in comparison to bone marrow-derived mesenchymal stem cells.
Stem Cells, June 1, 2006; 24(6): 1613 - 1619.
[Abstract] [Full Text] [PDF]


Home page
Stem CellsHome page
G.-R. Li, X.-L. Deng, H. Sun, S. S.M. Chung, H.-F. Tse, and C.-P. Lau
Ion Channels in Mesenchymal Stem Cells from Rat Bone Marrow
Stem Cells, June 1, 2006; 24(6): 1519 - 1528.
[Abstract] [Full Text] [PDF]


Home page
Stem CellsHome page
S. Kern, H. Eichler, J. Stoeve, H. Kluter, and K. Bieback
Comparative Analysis of Mesenchymal Stem Cells from Bone Marrow, Umbilical Cord Blood, or Adipose Tissue
Stem Cells, May 1, 2006; 24(5): 1294 - 1301.
[Abstract] [Full Text] [PDF]


Home page
Stem CellsHome page
Z. Guo, H. Li, X. Li, X. Yu, H. Wang, P. Tang, and N. Mao
In Vitro Characteristics and In Vivo Immunosuppressive Activity of Compact Bone-Derived Murine Mesenchymal Progenitor Cells
Stem Cells, April 1, 2006; 24(4): 992 - 1000.
[Abstract] [Full Text] [PDF]


Home page
aacredbookHome page
P. J. Simmons, B. Short, and N. Brouard
The Properties of Prospectively Isolated Mesenchymal Stem Cells from Man and Mouse
Am. Assoc. Cancer Res. Educ. Book, April 1, 2006; 2006(1): 334 - 338.
[Full Text] [PDF]


Home page
Stem CellsHome page
X. Wang, H. Hisha, S. Taketani, Y. Adachi, Q. Li, W. Cui, Y. Cui, J. Wang, C. Song, T. Mizokami, et al.
Characterization of Mesenchymal Stem Cells Isolated from Mouse Fetal Bone Marrow
Stem Cells, March 1, 2006; 24(3): 482 - 493.
[Abstract] [Full Text] [PDF]


Home page
Stem CellsHome page
D. G. Phinney, K. Hill, C. Michelson, M. DuTreil, C. Hughes, S. Humphries, R. Wilkinson, M. Baddoo, and E. Bayly
Biological Activities Encoded by the Murine Mesenchymal Stem Cell Transcriptome Provide a Basis for Their Developmental Potential and Broad Therapeutic Efficacy
Stem Cells, January 1, 2006; 24(1): 186 - 198.
[Abstract] [Full Text] [PDF]


Home page
BloodHome page
D. Baksh, J. E. Davies, and P. W. Zandstra
Soluble factor cross-talk between human bone marrow-derived hematopoietic and mesenchymal cells enhances in vitro CFU-F and CFU-O growth and reveals heterogeneity in the mesenchymal progenitor cell compartment
Blood, November 1, 2005; 106(9): 3012 - 3019.
[Abstract] [Full Text] [PDF]


Home page
Stem CellsHome page
A. Shahdadfar, K. Fronsdal, T. Haug, F. P. Reinholt, and J. E. Brinchmann
In Vitro Expansion of Human Mesenchymal Stem Cells: Choice of Serum Is a Determinant of Cell Proliferation, Differentiation, Gene Expression, and Transcriptome Stability
Stem Cells, September 1, 2005; 23(9): 1357 - 1366.
[Abstract] [Full Text] [PDF]


Home page
Mol. Biol. CellHome page
B. M. Deasy, B. M. Gharaibeh, J. B. Pollett, M. M. Jones, M. A. Lucas, Y. Kanda, and J. Huard
Long-Term Self-Renewal of Postnatal Muscle-derived Stem Cells
Mol. Biol. Cell, July 1, 2005; 16(7): 3323 - 3333.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
H. Egusa, F. E. Schweizer, C.-C. Wang, Y. Matsuka, and I. Nishimura
Neuronal Differentiation of Bone Marrow-derived Stromal Stem Cells Involves Suppression of Discordant Phenotypes through Gene Silencing
J. Biol. Chem., June 24, 2005; 280(25): 23691 - 23697.
[Abstract] [Full Text] [PDF]


Home page
Stem CellsHome page
D. Zipori
The Stem State: Plasticity Is Essential, Whereas Self-Renewal and Hierarchy Are Optional
Stem Cells, June 1, 2005; 23(6): 719 - 726.
[Abstract] [Full Text] [PDF]


Home page
Stem CellsHome page
G.-R. Li, H. Sun, X. Deng, and C.-P. Lau
Characterization of Ionic Currents in Human Mesenchymal Stem Cells from Bone Marrow
Stem Cells, March 1, 2005; 23(3): 371 - 382.
[Abstract] [Full Text] [PDF]


Home page
BloodHome page
S. Aggarwal and M. F. Pittenger
Human mesenchymal stem cells modulate allogeneic immune cell responses
Blood, February 15, 2005; 105(4): 1815 - 1822.
[Abstract] [Full Text] [PDF]


Home page
Stem CellsHome page
J. R. Smith, R. Pochampally, A. Perry, S.-C. Hsu, and D. J. Prockop
Isolation of a Highly Clonogenic and Multipotential Subfraction of Adult Stem Cells from Bone Marrow Stroma
Stem Cells, September 1, 2004; 22(5): 823 - 831.
[Abstract] [Full Text] [PDF]


Home page
Stem CellsHome page
S. L. Etheridge, G. J. Spencer, D. J. Heath, and P. G. Genever
Expression Profiling and Functional Analysis of Wnt Signaling Mechanisms in Mesenchymal Stem Cells
Stem Cells, September 1, 2004; 22(5): 849 - 860.
[Abstract] [Full Text] [PDF]


Home page
Circ. Res.Home page
M. F. Pittenger and B. J. Martin
Mesenchymal Stem Cells and Their Potential as Cardiac Therapeutics
Circ. Res., July 9, 2004; 95(1): 9 - 20.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
T. Aoyama, T. Okamoto, S. Nagayama, K. Nishijo, T. Ishibe, K. Yasura, T. Nakayama, T. Nakamura, and J. Toguchida
Methylation in the Core-promoter Region of the Chondromodulin-I Gene Determines the Cell-specific Expression by Regulating the Binding of Transcriptional Activator Sp3
J. Biol. Chem., July 2, 2004; 279(27): 28789 - 28797.
[Abstract] [Full Text] [PDF]


Home page
Stem CellsHome page
K. Bieback, S. Kern, H. Kluter, and H. Eichler
Critical Parameters for the Isolation of Mesenchymal Stem Cells from Umbilical Cord Blood
Stem Cells, July 1, 2004; 22(4): 625 - 634.
[Abstract] [Full Text] [PDF]


Home page
BloodHome page
G. Prindull and D. Zipori
Environmental guidance of normal and tumor cell plasticity: epithelial mesenchymal transitions as a paradigm
Blood, April 15, 2004; 103(8): 2892 - 2899.
[Abstract] [Full Text] [PDF]


Home page
EndocrinologyHome page
M. Heim, O. Frank, G. Kampmann, N. Sochocky, T. Pennimpede, P. Fuchs, W. Hunziker, P. Weber, I. Martin, and I. Bendik
The Phytoestrogen Genistein Enhances Osteogenesis and Represses Adipogenic Differentiation of Human Primary Bone Marrow Stromal Cells
Endocrinology, February 1, 2004; 145(2): 848 - 859.
[Abstract] [Full Text] [PDF]


Home page
J. Physiol.Home page
J. F. Heubach, E. M. Graf, J. Leutheuser, M. Bock, B. Balana, I. Zahanich, T. Christ, S. Boxberger, E. Wettwer, and U. Ravens
Electrophysiological properties of human mesenchymal stem cells
J. Physiol., February 1, 2004; 554(3): 659 - 672.
[Abstract] [Full Text] [PDF]


Home page
Stem CellsHome page
R. Cancedda, G. Bianchi, A. Derubeis, and R. Quarto
Cell Therapy for Bone Disease: A Review of Current Status
Stem Cells, September 1, 2003; 21(5): 610 - 619.
[Abstract] [Full Text] [PDF]


Home page
J. Neurosci.Home page
A. Kicic, W.-Y. Shen, A. S. Wilson, I. J. Constable, T. Robertson, and P. E. Rakoczy
Differentiation of Marrow Stromal Cells into Photoreceptors in the Rat Eye
J. Neurosci., August 27, 2003; 23(21): 7742 - 7749.
[Abstract] [Full Text] [PDF]


Home page
J. Cell Sci.Home page
A. Muraglia, A. Corsi, M. Riminucci, M. Mastrogiacomo, R. Cancedda, P. Bianco, and R. Quarto
Formation of a chondro-osseous rudiment in micromass cultures of human bone-marrow stromal cells
J. Cell Sci., July 15, 2003; 116(14): 2949 - 2955.
[Abstract] [Full Text] [PDF]


Home page
Cardiovasc ResHome page
Y. Liu, J. Song, W. Liu, Y. Wan, X. Chen, and C. Hu
Growth and differentiation of rat bone marrow stromal cells: does 5-azacytidine trigger their cardiomyogenic differentiation?
Cardiovasc Res, May 1, 2003; 58(2): 460 - 468.
[Abstract] [Full Text] [PDF]


Home page
Mol. Biol. CellHome page
P. A. Zuk, M. Zhu, P. Ashjian, D. A. De Ugarte, J. I. Huang, H. Mizuno, Z. C. Alfonso, J. K. Fraser, P. Benhaim, and M. H. Hedrick
Human Adipose Tissue Is a Source of Multipotent Stem Cells
Mol. Biol. Cell, December 1, 2002; 13(12): 4279 - 4295.
[Abstract] [Full Text] [PDF]


Home page
J. Cell Biol.Home page
J. Skillington, L. Choy, and R. Derynck
Bone morphogenetic protein and retinoic acid signaling cooperate to induce osteoblast differentiation of preadipocytes
J. Cell Biol., October 14, 2002; 159(1): 135 - 146.
[Abstract] [Full Text] [PDF]


Home page
Stem CellsHome page
J. E. Dennis and P. Charbord
Origin and Differentiation of Human and Murine Stroma
Stem Cells, May 1, 2002; 20(3): 205 - 214.
[Abstract] [Full Text] [PDF]


Home page
J. Cell Sci.Home page
C. Hegert, J. Kramer, G. Hargus, J. Muller, K. Guan, A. M. Wobus, P. K. Muller, and J. Rohwedel
Differentiation plasticity of chondrocytes derived from mouse embryonic stem cells
J. Cell Sci., January 12, 2002; 115(23): 4617 - 4628.
[Abstract] [Full Text] [PDF]


Home page
J. Leukoc. Biol.Home page
D. Zipori and M. Barda-Saad
Role of activin A in negative regulation of normal and tumor B lymphocytes
J. Leukoc. Biol., June 1, 2001; 69(6): 867 - 873.
[Abstract] [Full Text] [PDF]


Home page
Exp. Biol. Med.Home page
J. J. Minguell, A. Erices, and P. Conget
Mesenchymal Stem Cells
Experimental Biology and Medicine, June 1, 2001; 226(6): 507 - 520.
[Abstract] [Full Text] [PDF]




© The Company of Biologists Ltd 2000