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Journal of Cell Science, Vol 113, Issue 7 1231-1239, Copyright © 2000 by Company of Biologists

JOURNAL ARTICLES

Morphology and behaviour of dinoflagellate chromosomes during the cell cycle and mitosis

Y Bhaud, D Guillebault, J Lennon, H Defacque, MO Soyer-Gobillard and H Moreau
Observatoire Oceanologique de Banyuls, Laboratoire Arago, UMR CNRS 7628, BP44, F-66651 Banyuls-sur-Mer, France.

The morphology and behaviour of the chromosomes of dinoflagellates during the cell cycle appear to be unique among eukaryotes. We used synchronized and aphidicolin-blocked cultures of the dinoflagellate Crypthecodinium cohnii to describe the successive morphological changes that chromosomes undergo during the cell cycle. The chromosomes in early G(1) phase appeared to be loosely condensed with numerous structures protruding toward the nucleoplasm. They condensed in late G(1), before unwinding in S phase. The chromosomes in cells in G(2) phase were tightly condensed and had a double number of arches, as visualised by electron microscopy. During prophase, chromosomes elongated and split longitudinally, into characteristic V or Y shapes. We also used confocal microscopy to show a metaphase-like alignment of the chromosomes, which has never been described in dinoflagellates. The metaphase-like nucleus appeared flattened and enlarged, and continued to do so into anaphase. Chromosome segregation occurred via binding to the nuclear envelope surrounding the cytoplasmic channels and microtubule bundles. Our findings are summarized in a model of chromosome behaviour during the cell cycle.


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D. Guillebault, S. Sasorith, E. Derelle, J.-M. Wurtz, J.-C. Lozano, S. Bingham, L. Tora, and H. Moreau
A New Class of Transcription Initiation Factors, Intermediate between TATA Box-binding Proteins (TBPs) and TBP-like Factors (TLFs), Is Present in the Marine Unicellular Organism, the Dinoflagellate Crypthecodinium cohnii
J. Biol. Chem., October 18, 2002; 277(43): 40881 - 40886.
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