spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by McCarthy, K. M.
Right arrow Articles by Simister, N. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by McCarthy, K. M.
Right arrow Articles by Simister, N. E.

Journal of Cell Science, Vol 113, Issue 7 1277-1285, Copyright © 2000 by Company of Biologists

JOURNAL ARTICLES

Bidirectional transcytosis of IgG by the rat neonatal Fc receptor expressed in a rat kidney cell line: a system to study protein transport across epithelia

KM McCarthy, Y Yoong and NE Simister
Rosenstiel Center for Basic Biomedical Sciences, W.M. Keck Institute for Cellular Visualization, and Biology Department, Brandeis University, Waltham, MA 02254-9110, USA.

The neonatal Fc receptor, FcRn, transports immunoglobulin G (IgG) across cellular barriers between mother and offspring. FcRn also protects circulating IgG from catabolism, probably during transport across the capillary endothelium. Only one cell culture model of transcytosis has been used extensively, the transport of IgA from the basolateral to the apical surface of Madin-Darby canine kidney cells by the polymeric immunoglobulin receptor (pIgR). We report that rat inner medullary collecting duct (IMCD) cells transfected with DNA encoding the (alpha) subunit of rat FcRn specifically and saturably transport Fc when grown as polarized monolayers. Using this system, we have found that transcytosis by FcRn, like transcytosis by the pIgR, depends upon an intact microtubule system. FcRn differs most strikingly from the pIgR in its ability to transport its ligand in both the apical to basolateral and basolateral to apical directions. The phosphatidylinositol 3-kinase inhibitors wortmannin and LY294002 inhibited basolateral to apical transport by FcRn more than apical to basolateral transport, suggesting that there are differences in the mechanisms of transport in the two directions. Lastly, we found that transcytosis by FcRn depends upon vesicular acidification. We anticipate that the IMCD cell culture model will allow further elucidation of the mechanism of IgG transport by FcRn.


This article has been cited by other articles:


Home page
 J. Immunol.Home page
X. Liu, L. Ye, Y. Bai, H. Mojidi, N. E. Simister, and X. Zhu
Activation of the JAK/STAT-1 Signaling Pathway by IFN-{gamma} Can Down-Regulate Functional Expression of the MHC Class I-Related Neonatal Fc Receptor for IgG
J. Immunol., July 1, 2008; 181(1): 449 - 463.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
D. B. Tesar, E. J. Cheung, and P. J. Bjorkman
The Chicken Yolk Sac IgY Receptor, a Mammalian Mannose Receptor Family Member, Transcytoses IgY across Polarized Epithelial Cells
Mol. Biol. Cell, April 1, 2008; 19(4): 1587 - 1593.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Akilesh, G. J. Christianson, D. C. Roopenian, and A. S. Shaw
Neonatal FcR Expression in Bone Marrow-Derived Cells Functions to Protect Serum IgG from Catabolism
J. Immunol., October 1, 2007; 179(7): 4580 - 4588.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
P. Prabhat, Z. Gan, J. Chao, S. Ram, C. Vaccaro, S. Gibbons, R. J. Ober, and E. S. Ward
Elucidation of intracellular recycling pathways leading to exocytosis of the Fc receptor, FcRn, by using multifocal plane microscopy
PNAS, April 3, 2007; 104(14): 5889 - 5894.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
N. L. Harris, I. Spoerri, J. F. Schopfer, C. Nembrini, P. Merky, J. Massacand, J. F. Urban Jr., A. Lamarre, K. Burki, B. Odermatt, et al.
Mechanisms of Neonatal Mucosal Antibody Protection
J. Immunol., November 1, 2006; 177(9): 6256 - 6262.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
G. H. Hansen, L.-L. Niels-Christiansen, L. Immerdal, and E. M. Danielsen
Antibodies in the small intestine: mucosal synthesis and deposition of anti-glycosyl IgA, IgM, and IgG in the enterocyte brush border
Am J Physiol Gastrointest Liver Physiol, July 1, 2006; 291(1): G82 - G90.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
I. Kacskovics, Z. Kis, B. Mayer, A. P. West Jr, N. E. Tiangco, M. Tilahun, L. Cervenak, P. J. Bjorkman, R. A. Goldsby, O. Szenci, et al.
FcRn mediates elongated serum half-life of human IgG in cattle
Int. Immunol., April 1, 2006; 18(4): 525 - 536.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
E. Maidji, S. McDonagh, O. Genbacev, T. Tabata, and L. Pereira
Maternal Antibodies Enhance or Prevent Cytomegalovirus Infection in the Placenta by Neonatal Fc Receptor-Mediated Transcytosis
Am. J. Pathol., April 1, 2006; 168(4): 1210 - 1226.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
R. A. Knee, D. K. Hickey, K. W. Beagley, and R. C. Jones
Transport of IgG across the Blood-Luminal Barrier of the Male Reproductive Tract of the Rat and the Effect of Estradiol Administration on Reabsorption of Fluid and IgG by the Epididymal Ducts
Biol Reprod, October 1, 2005; 73(4): 688 - 694.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
E. E. Newton, Z. Wu, and N. E. Simister
Characterization of basolateral-targeting signals in the neonatal Fc receptor
J. Cell Sci., June 1, 2005; 118(11): 2461 - 2469.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
E. S. Ward, C. Martinez, C. Vaccaro, J. Zhou, Q. Tang, and R. J. Ober
From Sorting Endosomes to Exocytosis: Association of Rab4 and Rab11 GTPases with the Fc Receptor, FcRn, during Recycling
Mol. Biol. Cell, April 1, 2005; 16(4): 2028 - 2038.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. L. B. Wernick, V. Haucke, and N. E. Simister
Recognition of the Tryptophan-based Endocytosis Signal in the Neonatal Fc Receptor by the {micro} Subunit of Adaptor Protein-2
J. Biol. Chem., February 25, 2005; 280(8): 7309 - 7316.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
K.-J. Kim, T. E. Fandy, V. H. L. Lee, D. K. Ann, Z. Borok, and E. D. Crandall
Net absorption of IgG via FcRn-mediated transcytosis across rat alveolar epithelial cell monolayers
Am J Physiol Lung Cell Mol Physiol, September 1, 2004; 287(3): L616 - L622.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
R. J. Ober, C. Martinez, X. Lai, J. Zhou, and E. S. Ward
Exocytosis of IgG as mediated by the receptor, FcRn: An analysis at the single-molecule level
PNAS, July 27, 2004; 101(30): 11076 - 11081.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
S. M. Claypool, B. L. Dickinson, J. S. Wagner, F.-E. Johansen, N. Venu, J. A. Borawski, W. I. Lencer, and R. S. Blumberg
Bidirectional Transepithelial IgG Transport by a Strongly Polarized Basolateral Membrane Fc{gamma}-Receptor
Mol. Biol. Cell, April 1, 2004; 15(4): 1746 - 1759.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
L. Pereira, E. Maidji, S. McDonagh, O. Genbacev, and S. Fisher
Human Cytomegalovirus Transmission from the Uterus to the Placenta Correlates with the Presence of Pathogenic Bacteria and Maternal Immunity
J. Virol., December 15, 2003; 77(24): 13301 - 13314.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Sehra, G. Pynaert, K. Tournoy, A. Haegeman, P. Matthys, Y. Tagawa, R. Pauwels, and J. Grooten
Airway IgG Counteracts Specific and Bystander Allergen-Triggered Pulmonary Inflammation by a Mechanism Dependent on Fc{gamma}R and IFN-{gamma}
J. Immunol., August 15, 2003; 171(4): 2080 - 2089.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
P. L. TUMA and A. L. HUBBARD
Transcytosis: Crossing Cellular Barriers
Physiol Rev, July 1, 2003; 83(3): 871 - 932.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
D. C. Roopenian, G. J. Christianson, T. J. Sproule, A. C. Brown, S. Akilesh, N. Jung, S. Petkova, L. Avanessian, E. Y. Choi, D. J. Shaffer, et al.
The MHC Class I-Like IgG Receptor Controls Perinatal IgG Transport, IgG Homeostasis, and Fate of IgG-Fc-Coupled Drugs
J. Immunol., April 1, 2003; 170(7): 3528 - 3533.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
E. S. Ward, J. Zhou, V. Ghetie, and R. J. Ober
Evidence to support the cellular mechanism involved in serum IgG homeostasis in humans
Int. Immunol., February 1, 2003; 15(2): 187 - 195.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
K.-J. Kim and A. B. Malik
Protein transport across the lung epithelial barrier
Am J Physiol Lung Cell Mol Physiol, February 1, 2003; 284(2): L247 - L259.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Med.Home page
G. M. Spiekermann, P. W. Finn, E. S. Ward, J. Dumont, B. L. Dickinson, R. S. Blumberg, and W. I. Lencer
Receptor-mediated Immunoglobulin G Transport Across Mucosal Barriers in Adult Life: Functional Expression of FcRn in the Mammalian Lung
J. Exp. Med., August 5, 2002; 196(3): 303 - 310.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. M. Claypool, B. L. Dickinson, M. Yoshida, W. I. Lencer, and R. S. Blumberg
Functional Reconstitution of Human FcRn in Madin-Darby Canine Kidney Cells Requires Co-expressed Human beta 2-Microglobulin
J. Biol. Chem., July 26, 2002; 277(31): 28038 - 28050.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
C. Rauch, A.-C. Brunet, J. Deleule, and E. Farge
C2C12 myoblast/osteoblast transdifferentiation steps enhanced by epigenetic inhibition of BMP2 endocytosis
Am J Physiol Cell Physiol, July 1, 2002; 283(1): C235 - C243.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
R. J. Ober, C. G. Radu, V. Ghetie, and E. S. Ward
Differences in promiscuity for antibody-FcRn interactions across species: implications for therapeutic antibodies
Int. Immunol., December 1, 2001; 13(12): 1551 - 1559.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
X. Zhu, G. Meng, B. L. Dickinson, X. Li, E. Mizoguchi, L. Miao, Y. Wang, C. Robert, B. Wu, P. D. Smith, et al.
MHC Class I-Related Neonatal Fc Receptor for IgG Is Functionally Expressed in Monocytes, Intestinal Macrophages, and Dendritic Cells
J. Immunol., March 1, 2001; 166(5): 3266 - 3276.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
K. McCarthy, M Lam, L Subramanian, R Shakya, Z Wu, E. Newton, and N. Simister
Effects of mutations in potential phosphorylation sites on transcytosis of FcRn
J. Cell Sci., January 4, 2001; 114(8): 1591 - 1598.
[Abstract] [PDF]

Home page
 J. Biol. Chem.Home page
Z. Wu and N. E. Simister
Tryptophan- and Dileucine-based Endocytosis Signals in the Neonatal Fc Receptor
J. Biol. Chem., February 9, 2001; 276(7): 5240 - 5247.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
N. Kobayashi, Y. Suzuki, T. Tsuge, K. Okumura, C. Ra, and Y. Tomino
FcRn-mediated transcytosis of immunoglobulin G in human renal proximal tubular epithelial cells
Am J Physiol Renal Physiol, February 1, 2002; 282(2): F358 - F365.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2000