|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 113, Issue 7 1287-1298, Copyright © 2000 by Company of Biologists
JOURNAL ARTICLES |
RJ Eddy, LM Pierini, F Matsumura and FR Maxfield
Department of Biochemistry, Weill Medical College of Cornell University, New York, NY, USA.
Buffering of intracellular Ca2+ transients in human neutrophils leads to reduced motility due to defective uropod detachment on fibronectin and vitronectin-coated surfaces. Since one potential target of a rise in [Ca2+]i is the activation of myosin II, we characterized the role of myosin II during motility. Treatment of neutrophils with a myosin inhibitor (2,3-butanedione monoxime), or myosin light chain kinase inhibitors (ML-7, ML-9, or KT5926) resulted in impaired uropod retraction and a dose-dependent decrease in chemokinesis following stimulation with N-formyl-Met-Leu-Phe (fMLP). Treatment with ML-9 resulted in a redistribution of F-actin and talin to the non-retracted uropods, mimicking the redistribution observed during [Ca2+]i buffering. Impairment of uropod retraction and redistribution of F-actin and talin by myosin II inhibition was only observed on adhesive substrates such as fibronectin and not on poorly adhesive substrates such as human serum-coated glass. At higher concentrations of ML-9, cell polarization was inhibited and pseudopod extension occurred radially. Using an antibody specific for serine 19-phosphorylated regulatory light chain of myosin II, regions of activated myosin II were found at the leading edge as well as the uropod in motile fMLP-stimulated cells. [Ca2+]i depletion caused a 50% decrease in the level of serine 19-phosphorylated myosin II suggesting that activation of myosin II by intracellular Ca2+ transients may be an essential step in establishing a polarized pseudopod and providing the force required for uropod retraction during PMN motility on adhesive surfaces.
This article has been cited by other articles:
|
|
 |
|
  C. D. Chilcoat, Y. Sharief, and S. L. Jones Tonic protein kinase A activity maintains inactive {beta}2 integrins in unstimulated neutrophils by reducing myosin light-chain phosphorylation: role of myosin light-chain kinase and Rho kinase J. Leukoc. Biol., April 1, 2008; 83(4): 964 - 971. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Abou-Kheir, B. Isaac, H. Yamaguchi, and D. Cox Membrane targeting of WAVE2 is not sufficient for WAVE2-dependent actin polymerization: a role for IRSp53 in mediating the interaction between Rac and WAVE2 J. Cell Sci., February 1, 2008; 121(3): 379 - 390. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. F. Coughlin, D. D. Sohn, and G. W. Schmid-Schonbein Recoil and Stiffening by Adherent Leukocytes in Response to Fluid Shear Biophys. J., February 1, 2008; 94(3): 1046 - 1051. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Conti and R. S. Adelstein Nonmuscle myosin II moves in new directions J. Cell Sci., January 1, 2008; 121(1): 11 - 18. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Lokuta, M. A. Senetar, D. A. Bennin, P. A. Nuzzi, K. T. Chan, V. L. Ott, and A. Huttenlocher Type I{gamma} PIP Kinase Is a Novel Uropod Component that Regulates Rear Retraction during Neutrophil Chemotaxis Mol. Biol. Cell, December 1, 2007; 18(12): 5069 - 5080. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Samaniego, L. Sanchez-Martin, A. Estecha, and P. Sanchez-Mateos Rho/ROCK and myosin II control the polarized distribution of endocytic clathrin structures at the uropod of moving T lymphocytes J. Cell Sci., October 15, 2007; 120(20): 3534 - 3543. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. El-Sibai, P. Nalbant, H. Pang, R. J. Flinn, C. Sarmiento, F. Macaluso, M. Cammer, J. S. Condeelis, K. M. Hahn, and J. M. Backer Cdc42 is required for EGF-stimulated protrusion and motility in MTLn3 carcinoma cells J. Cell Sci., October 1, 2007; 120(19): 3465 - 3474. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Hopkins, A. A. Pineda, L. M. Winfree, G. T. Brown, M. G. Laukoetter, and A. Nusrat Organized migration of epithelial cells requires control of adhesion and protrusion through Rho kinase effectors Am J Physiol Gastrointest Liver Physiol, March 1, 2007; 292(3): G806 - G817. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.-D. Filippi, K. Szczur, C. E. Harris, and P.-Y. Berclaz Rho GTPase Rac1 is critical for neutrophil migration into the lung Blood, February 1, 2007; 109(3): 1257 - 1264. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Campello, R. A. Lacalle, M. Bettella, S. Manes, L. Scorrano, and A. Viola Orchestration of lymphocyte chemotaxis by mitochondrial dynamics J. Exp. Med., December 25, 2006; 203(13): 2879 - 2886. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. C. Sandquist, K. I. Swenson, K. A. DeMali, K. Burridge, and A. R. Means Rho Kinase Differentially Regulates Phosphorylation of Nonmuscle Myosin II Isoforms A and B during Cell Rounding and Migration J. Biol. Chem., November 24, 2006; 281(47): 35873 - 35883. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Kolega The Role of Myosin II Motor Activity in Distributing Myosin Asymmetrically and Coupling Protrusive Activity to Cell Translocation Mol. Biol. Cell, October 1, 2006; 17(10): 4435 - 4445. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Song, X. Chen, H. Yamaguchi, G. Mouneimne, J. S. Condeelis, and R. J. Eddy Initiation of cofilin activity in response to EGF is uncoupled from cofilin phosphorylation and dephosphorylation in carcinoma cells J. Cell Sci., July 15, 2006; 119(14): 2871 - 2881. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Bastian, B. Posch, K. Lang, B. Niggemann, K. S. Zaenker, H. Hatt, and F. Entschladen Phosphatidylinositol 3-Kinase in the G Protein-Coupled Receptor-Induced Chemokinesis and Chemotaxis of MDA-MB-468 Breast Carcinoma Cells: A Comparison with Leukocytes Mol. Cancer Res., June 1, 2006; 4(6): 411 - 421. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. J. Evans, A. McDowall, P. C. Taylor, N. Hogg, D. O. Haskard, and R. C. Landis Shedding of lymphocyte function-associated antigen-1 (LFA-1) in a human inflammatory response Blood, May 1, 2006; 107(9): 3593 - 3599. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Bodin and M. D. Welch Plasma Membrane Organization Is Essential for Balancing Competing Pseudopod- and Uropod-promoting Signals during Neutrophil Polarization and Migration Mol. Biol. Cell, December 1, 2005; 16(12): 5773 - 5783. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Verploegen, L. Ulfman, H. W. M. van Deutekom, C. van Aalst, H. Honing, J.-W. J. Lammers, L. Koenderman, and P. J. Coffer Characterization of the role of CaMKI-like kinase (CKLiK) in human granulocyte function Blood, August 1, 2005; 106(3): 1076 - 1083. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Yang and X.-Y. Huang Ca2+ Influx through L-type Ca2+ Channels Controls the Trailing Tail Contraction in Growth Factor-induced Fibroblast Cell Migration J. Biol. Chem., July 22, 2005; 280(29): 27130 - 27137. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. J. Dixon and P. J. Roy Muscle arm development in Caenorhabditis elegans Development, July 1, 2005; 132(13): 3079 - 3092. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. D. Doyle and J. Lee Cyclic changes in keratocyte speed and traction stress arise from Ca2+-dependent regulation of cell adhesiveness J. Cell Sci., January 15, 2005; 118(2): 369 - 379. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Franca-Koh and P. N. Devreotes Moving Forward: Mechanisms of Chemoattractant Gradient Sensing Physiology, October 1, 2004; 19(5): 300 - 308. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. F. Coughlin and G. W. Schmid-Schonbein Pseudopod Projection and Cell Spreading of Passive Leukocytes in Response to Fluid Shear Stress Biophys. J., September 1, 2004; 87(3): 2035 - 2042. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Giannone, P. Ronde, M. Gaire, J. Beaudouin, J. Haiech, J. Ellenberg, and K. Takeda Calcium Rises Locally Trigger Focal Adhesion Disassembly and Enhance Residency of Focal Adhesion Kinase at Focal Adhesions J. Biol. Chem., July 2, 2004; 279(27): 28715 - 28723. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Doyle, W. Marganski, and J. Lee Calcium transients induce spatially coordinated increases in traction force during the movement of fish keratocytes J. Cell Sci., May 1, 2004; 117(11): 2203 - 2214. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Eisen, D. R. Ratcliffe, and G. K. Ojakian Modulation of epithelial tubule formation by Rho kinase Am J Physiol Cell Physiol, April 1, 2004; 286(4): C857 - C866. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-M. Lo, D. B. Buxton, G. C.H. Chua, M. Dembo, R. S. Adelstein, and Y.-L. Wang Nonmuscle Myosin IIB Is Involved in the Guidance of Fibroblast Migration Mol. Biol. Cell, March 1, 2004; 15(3): 982 - 989. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Totsukawa, Y. Wu, Y. Sasaki, D. J. Hartshorne, Y. Yamakita, S. Yamashiro, and F. Matsumura Distinct roles of MLCK and ROCK in the regulation of membrane protrusions and focal adhesion dynamics during cell migration of fibroblasts J. Cell Biol., February 2, 2004; 164(3): 427 - 439. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. Rogers, U. Wiedemann, N. Stuurman, and R. D. Vale Molecular requirements for actin-based lamella formation in Drosophila S2 cells J. Cell Biol., September 15, 2003; 162(6): 1079 - 1088. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Kempiak, S.-C. Yip, J. M. Backer, and J. E. Segall Local signaling by the EGF receptor J. Cell Biol., September 1, 2003; 162(5): 781 - 788. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Zhou, C. Watson, C. Fu, X. Yao, and J. G. Forte Myosin II is present in gastric parietal cells and required for lamellipodial dynamics associated with cell activation Am J Physiol Cell Physiol, September 1, 2003; 285(3): C662 - C673. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Smith, M. Bracke, B. Leitinger, J. C. Porter, and N. Hogg LFA-1-induced T cell migration on ICAM-1 involves regulation of MLCK-mediated attachment and ROCK-dependent detachment J. Cell Sci., August 1, 2003; 116(15): 3123 - 3133. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. H. Adamson, M. Zeng, G. N. Adamson, J. F. Lenz, and F. E. Curry PAF- and bradykinin-induced hyperpermeability of rat venules is independent of actin-myosin contraction Am J Physiol Heart Circ Physiol, June 5, 2003; 285(1): H406 - H417. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Worthylake and K. Burridge RhoA and ROCK Promote Migration by Limiting Membrane Protrusions J. Biol. Chem., April 4, 2003; 278(15): 13578 - 13584. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. M. Pierini, R. J. Eddy, M. Fuortes, S. Seveau, C. Casulo, and F. R. Maxfield Membrane Lipid Organization Is Critical for Human Neutrophil Polarization J. Biol. Chem., March 14, 2003; 278(12): 10831 - 10841. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Samstag, S. M. Eibert, M. Klemke, and G. H. Wabnitz Actin cytoskeletal dynamics in T lymphocyte activation and migration J. Leukoc. Biol., January 1, 2003; 73(1): 30 - 48. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Furukawa, A. Maselli, S. A. M. Thomson, R. W. L. Lim, J. V. Stokes, and M. Fechheimer Calcium regulation of actin crosslinking is important for function of the actin cytoskeleton in Dictyostelium J. Cell Sci., January 1, 2003; 116(1): 187 - 196. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Eddy, L. M. Pierini, and F. R. Maxfield Microtubule Asymmetry during Neutrophil Polarization and Migration Mol. Biol. Cell, December 1, 2002; 13(12): 4470 - 4483. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Giannone, P. Ronde, M. Gaire, J. Haiech, and K. Takeda Calcium Oscillations Trigger Focal Adhesion Disassembly in Human U87 Astrocytoma Cells J. Biol. Chem., July 12, 2002; 277(29): 26364 - 26371. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Cicchetti, P. G. Allen, and M. Glogauer CHEMOTACTIC SIGNALING PATHWAYS IN NEUTROPHILS: FROM RECEPTOR TO ACTIN ASSEMBLY Crit. Rev. Oral. Biol. Med., May 1, 2002; 13(3): 220 - 228. [Abstract] [Full Text]
|
|
|
|
|
|
S. Ueda, M. Mizuki, H. Ikeda, T. Tsujimura, I. Matsumura, K. Nakano, H. Daino, Z.-i. Honda, J. Sonoyama, H. Shibayama, et al. Critical roles of c-Kit tyrosine residues 567 and 719 in stem cell factor-induced chemotaxis: contribution of src family kinase and PI3-kinase on calcium mobilization and cell migration Blood, May 1, 2002; 99(9): 3342 - 3349. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Y. Cho and R. L. Klemke Purification of pseudopodia from polarized cells reveals redistribution and activation of Rac through assembly of a CAS/Crk scaffold J. Cell Biol., February 18, 2002; 156(4): 725 - 736. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Seveau, R. J. Eddy, F. R. Maxfield, and L. M. Pierini Cytoskeleton-dependent Membrane Domain Segregation during Neutrophil Polarization Mol. Biol. Cell, November 1, 2001; 12(11): 3550 - 3562. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Worthylake, S. Lemoine, J. M. Watson, and K. Burridge RhoA is required for monocyte tail retraction during transendothelial migration J. Cell Biol., July 9, 2001; 154(1): 147 - 160. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Y. Cho and R. L. Klemke Purification of pseudopodia from polarized cells reveals redistribution and activation of Rac through assembly of a CAS/Crk scaffold J. Cell Biol., February 18, 2002; 156(4): 725 - 736. [Abstract] [Full Text] [PDF]
|
|
