spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hood, J. K.
Right arrow Articles by Silver, P. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hood, J. K.
Right arrow Articles by Silver, P. A.

Journal of Cell Science, Vol 113, Issue 8 1471-1480, Copyright © 2000 by Company of Biologists

JOURNAL ARTICLES

Nup2p is located on the nuclear side of the nuclear pore complex and coordinates Srp1p/importin-alpha export

JK Hood, JM Casolari and PA Silver
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School and The Dana-Farber Cancer Institute, Boston, MA 02115, USA.

Proteins bearing canonical nuclear localization sequences are imported into the nucleus by the importin/karyopherin-alpha/beta heterodimer. Recycling of the importin-alpha subunit to the cytoplasm requires the action of Cas, a member of the importin-beta superfamily. In the yeast Saccharomyces ceresivisiae, the essential gene CSE1 encodes a Cas homologue that exports the yeast importin-alpha protein Srp1p/Kap60p from the nucleus. In this report, we describe a role for the FXFG nucleoporin Nup2p, and possibly the related Nup1p, in the Cse1p-mediated nuclear export pathway. Yeast cells lacking Nup2p or containing a particular temperature-sensitive mutation in NUP1 accumulate Srp1p in the nucleus. Similarly, Cse1p is displaced from the nuclear rim to the nuclear interior in deltanup2 cells. We do not observe any biochemical interaction between Cse1p and Nup2p. Instead, we find that Nup2p binds directly to Srp1p. We have localized Nup2p to the interior face of the nuclear pore complex, and have shown that its N terminus is sufficient for targeting Nup2p to the pore, as well as for binding to Srp1p. Taken together, these data suggest that Nup2p is an important NPC docking site in the Srp1p export pathway.


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
C. Sun, W. Yang, L.-C. Tu, and S. M. Musser
Single-molecule measurements of importin {alpha}/cargo complex dissociation at the nuclear pore
PNAS, June 24, 2008; 105(25): 8613 - 8618.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
A. H. Osmani, J. Davies, H.-L. Liu, A. Nile, and S. A. Osmani
Systematic Deletion and Mitotic Localization of the Nuclear Pore Complex Proteins of Aspergillus nidulans
Mol. Biol. Cell, December 1, 2006; 17(12): 4946 - 4961.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. L. Miller, M. Suntharalingam, S. L. Johnson, A. Audhya, S. D. Emr, and S. R. Wente
Cytoplasmic Inositol Hexakisphosphate Production Is Sufficient for Mediating the Gle1-mRNA Export Pathway
J. Biol. Chem., December 3, 2004; 279(49): 51022 - 51032.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Gilchrist and M. Rexach
Molecular Basis for the Rapid Dissociation of Nuclear Localization Signals from Karyopherin {alpha} in the Nucleoplasm
J. Biol. Chem., December 19, 2003; 278(51): 51937 - 51949.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. Pyhtila and M. Rexach
A Gradient of Affinity for the Karyopherin Kap95p along the Yeast Nuclear Pore Complex
J. Biol. Chem., October 24, 2003; 278(43): 42699 - 42709.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. T. Harreman, P. E. Cohen, M. R. Hodel, G. J. Truscott, A. H. Corbett, and A. E. Hodel
Characterization of the Auto-inhibitory Sequence within the N-terminal Domain of Importin {alpha}
J. Biol. Chem., June 6, 2003; 278(24): 21361 - 21369.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
D. P. Denning, S. S. Patel, V. Uversky, A. L. Fink, and M. Rexach
Disorder in the nuclear pore complex: The FG repeat regions of nucleoporins are natively unfolded
PNAS, March 4, 2003; 100(5): 2450 - 2455.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. T. Harreman, M. R. Hodel, P. Fanara, A. E. Hodel, and A. H. Corbett
The Auto-inhibitory Function of Importin alpha Is Essential in Vivo
J. Biol. Chem., February 14, 2003; 278(8): 5854 - 5863.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Stade, F. Vogel, I. Schwienhorst, B. Meusser, C. Volkwein, B. Nentwig, R. J. Dohmen, and T. Sommer
A Lack of SUMO Conjugation Affects cNLS-dependent Nuclear Protein Import in Yeast
J. Biol. Chem., December 13, 2002; 277(51): 49554 - 49561.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. P. Denning, V. Uversky, S. S. Patel, A. L. Fink, and M. Rexach
The Saccharomyces cerevisiae Nucleoporin Nup2p Is a Natively Unfolded Protein
J. Biol. Chem., August 30, 2002; 277(36): 33447 - 33455.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
E. D. Schwoebel, T. H. Ho, and M. S. Moore
The mechanism of inhibition of Ran-dependent nuclear transport by cellular ATP depletion
J. Cell Biol., June 10, 2002; 157(6): 963 - 974.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Gilchrist, B. Mykytka, and M. Rexach
Accelerating the Rate of Disassembly of Karyopherin{middle dot}Cargo Complexes
J. Biol. Chem., May 10, 2002; 277(20): 18161 - 18172.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
M. Kunzler and E. Hurt
Targeting of Ran: variation on a common theme?
J. Cell Sci., March 11, 2002; 114(18): 3233 - 3241.
[Abstract] [Full Text] [PDF]

Home page
 Microbiol. Mol. Biol. Rev.Home page
I. G. Macara
Transport into and out of the Nucleus
Microbiol. Mol. Biol. Rev., December 1, 2001; 65(4): 570 - 594.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
D. Denning, B. Mykytka, N. P.C. Allen, L. Huang, Al Burlingame, and M. Rexach
The nucleoporin Nup60p functions as a Gsp1p-GTP-sensitive tether for Nup2p at the nuclear pore complex
J. Cell Biol., September 3, 2001; 154(5): 937 - 950.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
D. J. Dilworth, A. Suprapto, J. C. Padovan, B. T. Chait, R. W. Wozniak, M. P. Rout, and J. D. Aitchison
Nup2p Dynamically Associates with the Distal Regions of the Yeast Nuclear Pore Complex
J. Cell Biol., June 25, 2001; 153(7): 1465 - 1478.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
K. Geles and S. Adam
Germline and developmental roles of the nuclear transport factor importin (&agr;)3 in C. elegans
Development, January 5, 2001; 128(10): 1817 - 1830.
[Abstract] [PDF]

Home page
 Mol. Cell. Biol.Home page
J. Solsbacher, P. Maurer, F. Vogel, and G. Schlenstedt
Nup2p, a Yeast Nucleoporin, Functions in Bidirectional Transport of Importin alpha
Mol. Cell. Biol., November 15, 2000; 20(22): 8468 - 8479.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
L. A. Strawn, T. Shen, and S. R. Wente
The GLFG Regions of Nup116p and Nup100p Serve as Binding Sites for Both Kap95p and Mex67p at the Nuclear Pore Complex
J. Biol. Chem., February 23, 2001; 276(9): 6445 - 6452.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. P. C. Allen, L. Huang, A. Burlingame, and M. Rexach
Proteomic Analysis of Nucleoporin Interacting Proteins
J. Biol. Chem., July 27, 2001; 276(31): 29268 - 29274.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2000