|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 113, Issue 9 1543-1552, Copyright © 2000 by Company of Biologists
JOURNAL ARTICLES |
J Liu, MD Hebert, Y Ye, DJ Templeton, H Kung and AG Matera
Departments of Molecular Biology & Microbiology, Pathology Case Western Reserve University and University Hospitals of Cleveland, Cleveland, OH 44106, USA.
We have found that CDK2 and cyclin E, but not cyclin A, accumulates within Cajal bodies (CBs) in a cell cycle-dependent fashion. In the absence of cyclin E, CDK2 is not enriched in the CB compartment, suggesting that the translocation of CDK2 to CBs is dependent on cyclin E. CDK2 and cyclin E could be recruited to CBs as a functional complex or CBs may serve as 'docking stations' for CDK2-cyclin E activation by CAKs during the G(1)/S transition. Notably, CDK7-cyclin H-Mat1 complexes are known to accumulate in CBs. Treatment of cells with inhibitors of either CDKs (olomoucine, 200 microM) or RNA polymerase I (actinomycin D, 0.05 microgram/ml), results in a striking reorganization of CDK2 and p80 coilin to the nucleolar periphery. Furthermore, we demonstrate that p80 coilin can be phosphorylated by purified CDK2-cyclin E complexes in vitro. Thus coilin and other CB proteins appear to be downstream targets of CDK2-cyclin E complex-mediated signaling pathways regulating cell cycle progression and controlling aspects of CB function. Possible roles for CDK2 and cyclin E in the well-documented association of CBs, histone gene clusters and RNA 3' end processing factors are discussed.
This article has been cited by other articles:
|
|
 |
|
  J.-L. Liu, Z. Mao, T. A. LaFortune, M. M. Alonso, G. E. Gallick, J. Fueyo, and W.K. A. Yung Cell Cycle Dependent Nuclear Export of Phosphatase and Tensin Homologue Tumor Suppressor Is Regulated by the Phosphoinositide-3-Kinase Signaling Cascade Cancer Res., November 15, 2007; 67(22): 11054 - 11063. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. E. White, M. E. Leslie, B. R. Calvi, W. F. Marzluff, and R. J. Duronio Developmental and Cell Cycle Regulation of the Drosophila Histone Locus Body Mol. Biol. Cell, July 1, 2007; 18(7): 2491 - 2502. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Barcaroli, L. Bongiorno-Borbone, A. Terrinoni, T. G. Hofmann, M. Rossi, R. A. Knight, A. G. Matera, G. Melino, and V. De Laurenzi FLASH is required for histone transcription and S-phase progression PNAS, October 3, 2006; 103(40): 14808 - 14812. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-L. Liu, C. Murphy, M. Buszczak, S. Clatterbuck, R. Goodman, and J. G. Gall The Drosophila melanogaster Cajal body. J. Cell Biol., March 13, 2006; 172(6): 875 - 884. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Li, K. Roy, S. Katyal, X. Sun, S. Bleoo, and R. Godbout Dynamic Nature of Cleavage Bodies and Their Spatial Relationship to DDX1 Bodies, Cajal Bodies, and Gems Mol. Biol. Cell, March 1, 2006; 17(3): 1126 - 1140. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-L. Liu, X. Sheng, Z. K. Hortobagyi, Z. Mao, G. E. Gallick, and W. K. A. Yung Nuclear PTEN-Mediated Growth Suppression Is Independent of Akt Down-Regulation Mol. Cell. Biol., July 15, 2005; 25(14): 6211 - 6224. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Shav-Tal, J. Blechman, X. Darzacq, C. Montagna, B. T. Dye, J. G. Patton, R. H. Singer, and D. Zipori Dynamic Sorting of Nuclear Components into Distinct Nucleolar Caps during Transcriptional Inhibition Mol. Biol. Cell, May 1, 2005; 16(5): 2395 - 2413. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Rajaram and T. K. Kerppola Synergistic Transcription Activation by Maf and Sox and Their Subnuclear Localization Are Disrupted by a Mutation in Maf That Causes Cataract Mol. Cell. Biol., July 1, 2004; 24(13): 5694 - 5709. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Dundr, M. D. Hebert, T. S. Karpova, D. Stanek, H. Xu, K. B. Shpargel, U. T. Meier, K. M. Neugebauer, A. G. Matera, and T. Misteli In vivo kinetics of Cajal body components J. Cell Biol., March 15, 2004; 164(6): 831 - 842. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Palko, H. W. Bass, M. J. Beyrouthy, and M. M. Hurt The Yin Yang-1 (YY1) protein undergoes a DNA-replication-associated switch in localization from the cytoplasm to the nucleus at the onset of S phase J. Cell Sci., January 22, 2004; 117(3): 465 - 476. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Zhu, R. L. Tomlinson, A. A. Lukowiak, R. M. Terns, and M. P. Terns Telomerase RNA Accumulates in Cajal Bodies in Human Cancer Cells Mol. Biol. Cell, January 1, 2004; 15(1): 81 - 90. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Ye, Y. Wei, G. Nalepa, and J. W. Harper The Cyclin E/Cdk2 Substrate p220NPAT Is Required for S-Phase Entry, Histone Gene Expression, and Cajal Body Maintenance in Human Somatic Cells Mol. Cell. Biol., December 1, 2003; 23(23): 8586 - 8600. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Levy, Y. Izumiya, P. Brunovskis, L. Xia, M. S. Parcells, S. M. Reddy, L. Lee, H.-W. Chen, and H.-J. Kung Characterization of the Chromosomal Binding Sites and Dimerization Partners of the Viral Oncoprotein Meq in Marek's Disease Virus-Transformed T Cells J. Virol., December 1, 2003; 77(23): 12841 - 12851. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. E. Polak, F. Simone, J. J. Kaberlein, R. T. Luo, and M. J. Thirman ELL and EAF1 are Cajal Body Components That Are Disrupted in MLL-ELL Leukemia Mol. Biol. Cell, April 1, 2003; 14(4): 1517 - 1528. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. B. Shpargel, J. K. Ospina, K. E. Tucker, A. G. Matera, and M. D. Hebert Control of Cajal body number is mediated by the coilin C-terminus J. Cell Sci., January 15, 2003; 116(2): 303 - 312. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. W. Lam, C. E. Lyon, and A. I. Lamond Large-Scale Isolation of Cajal Bodies from HeLa Cells Mol. Biol. Cell, July 1, 2002; 13(7): 2461 - 2473. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Lefebvre, P. Burlet, L. Viollet, S. Bertrandy, C. Huber, C. Belser, and A. Munnich A novel association of the SMN protein with two major non-ribosomal nucleolar proteins and its implication in spinal muscular atrophy Hum. Mol. Genet., May 1, 2002; 11(9): 1017 - 1027. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Chen, T. Wurm, P. Britton, G. Brooks, and J. A. Hiscox Interaction of the Coronavirus Nucleoprotein with Nucleolar Antigens and the Host Cell J. Virol., April 16, 2002; 76(10): 5233 - 5250. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J. Young, K. T. Jensen, L. R. Burger, D. J. Pintel, and C. L. Lorson Minute Virus of Mice NS1 Interacts with the SMN Protein, and They Colocalize in Novel Nuclear Bodies Induced by Parvovirus Infection J. Virol., March 19, 2002; 76(8): 3892 - 3904. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Wurm, H. Chen, T. Hodgson, P. Britton, G. Brooks, and J. A. Hiscox Localization to the Nucleolus Is a Common Feature of Coronavirus Nucleoproteins, and the Protein May Disrupt Host Cell Division J. Virol., October 1, 2001; 75(19): 9345 - 9356. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. S. Shopland, M. Byron, J. L. Stein, J. B. Lian, G. S. Stein, and J. B. Lawrence Replication-dependent Histone Gene Expression Is Related to Cajal Body (CB) Association but Does Not Require Sustained CB Contact Mol. Biol. Cell, March 1, 2001; 12(3): 565 - 576. [Abstract] [Full Text]
|
|
|
|
|
|
M. D. Hebert and A. G. Matera Self-association of Coilin Reveals a Common Theme in Nuclear Body Localization Mol. Biol. Cell, December 1, 2000; 11(12): 4159 - 4171. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. Zhao, B. K. Kennedy, B. D. Lawrence, D. A. Barbie, A. G. Matera, J. A. Fletcher, and E. Harlow NPAT links cyclin E-Cdk2 to the regulation of replication-dependent histone gene transcription Genes & Dev., September 15, 2000; 14(18): 2283 - 2297. [Abstract] [Full Text]
|
|
|
|
|
|
T. Ma, B. A. Van Tine, Y. Wei, M. D. Garrett, D. Nelson, P. D. Adams, J. Wang, J. Qin, L. T. Chow, and J. W. Harper Cell cycle-regulated phosphorylation of p220NPAT by cyclin E/Cdk2 in Cajal bodies promotes histone gene transcription Genes & Dev., September 15, 2000; 14(18): 2298 - 2313. [Abstract] [Full Text]
|
|
