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Journal of Cell Science, Vol 113, Issue 9 1565-1576, Copyright © 2000 by Company of Biologists

JOURNAL ARTICLES

Large-scale chromatin organization of the major histocompatibility complex and other regions of human chromosome 6 and its response to interferon in interphase nuclei

EV Volpi, E Chevret, T Jones, R Vatcheva, J Williamson, S Beck, RD Campbell, M Goldsworthy, SH Powis, J Ragoussis, J Trowsdale and D Sheer
Human Cytogenetics Laboratory, Imperial Cancer Research Fund, London WC2A 3PX, UK.

The large-scale chromatin organization of the major histocompatibility complex and other regions of chromosome 6 was studied by three-dimensional image analysis in human cell types with major differences in transcriptional activity. Entire gene clusters were visualized by fluorescence in situ hybridization with multiple locus-specific probes. Individual genomic regions showed distinct configurations in relation to the chromosome 6 terrritory. Large chromatin loops containing several megabases of DNA were observed extending outwards from the surface of the domain defined by the specific chromosome 6 paint. The frequency with which a genomic region was observed on an external chromatin loop was cell type dependent and appeared to be related to the number of active genes in that region. Transcriptional up-regulation of genes in the major histocompatibility complex by interferon-gamma led to an increase in the frequency with which this large gene cluster was found on an external chromatin loop. Our data are consistent with an association between large-scale chromatin organization of specific genomic regions and their transcriptional status.


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