|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 114, Issue 1 131-139, Copyright © 2001 by Company of Biologists
JOURNAL ARTICLES |
YP Han, TL Tuan, H Wu, M Hughes and WL Garner
Division of Plastic and Reconstructive Surgery, University of Southern California School of Medicine, Los Angeles, California 90033, USA.
Tumor necrosis factor-alpha (TNF-(alpha)) is an important mediator during the inflammatory phase of wound healing. Excessive amounts of pro-inflammatory cytokines such as TNF-(alpha) are associated with inflammatory diseases including chronic wounds. Matrix metalloproteinases (MMPs) are involved in matrix re-modeling during wound healing, angiogenesis and tumor metastasis. As with pro-inflammatory cytokines, high levels of MMPs have been found in inflammatory states such as chronic wounds. In this report we relate these two phenomena. TNF-(alpha) stimulates secretion of active MMP-2, a type IV collagenase, in organ-cultured full-thickness human skin. This suggests a mechanism whereby excess inflammation affects normal wound healing. To investigate this observation at the cellular and molecular levels, we examined TNF-(alpha) mediated activation of pro-MMP-2, induction of MT1-MMP, and the intracellular signaling pathways that regulate the proteinase in isolated human dermal fibroblasts. We found that TNF-(alpha) substantially promoted activation of pro-MMP-2 in dermal fibroblasts embedded in type-I collagen. In marked contrast, collagen or TNF-(alpha) individually had little influence on the fibroblast-mediated pro-MMP-2 activation. One well-characterized mechanism for pro-MMP-2 activation is through a membrane type matrix metalloproteinase, such as MT1-MMP. We report that TNF-(alpha) significantly induced MT1-MMP at the mRNA and protein levels when the dermal fibroblasts were grown in collagen. Although the intracellular signaling pathway regulating mt1-mmp gene expression is still obscure, both TNF-(alpha) and collagen activate the NF-(kappa)B pathway. In this report we provide three sets of evidence to support a hypothesis that activation of NF-(kappa)B is essential to induce MT1-MMP expression in fibroblasts after TNF-(alpha) exposure. First, SN50, a peptide inhibitor for NF-(kappa)B nuclear translocation, simultaneously blocked the TNF-(alpha) and collagen mediated MT1-MMP induction and pro-MMP-2 activation. Secondly, TNF-(alpha) induced I(kappa)B to breakdown in fibroblasts within the collagen lattice, a critical step leading to NF-(kappa)B activation. Lastly, a consensus binding site for p65 NF-(kappa)B (TGGAGCTTCC) was found in the 5'-flanking region of human mt1-mmp gene. Based on these results and previous reports, we propose a model to explain TNF-(alpha) activation of MMP-2 in human skin. Activation of NF(kappa)B signaling in fibroblasts embedded in collagen induces mt1-mmp gene expression, which subsequently activates the pro-MMP-2. The findings provide a specific mechanism whereby TNF-(alpha) may affect matrix remodeling during wound healing and other physiological and pathological processes.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  W. Xiong, J. MacTaggart, R. Knispel, J. Worth, Y. Persidsky, and B. T. Baxter Blocking TNF-{alpha} Attenuates Aneurysm Formation in a Murine Model J. Immunol., August 15, 2009; 183(4): 2741 - 2746. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. H. Park, B.-H. Ahn, Y.-K. Hong, and D. S. Min Overexpression of phospholipase D enhances matrix metalloproteinase-2 expression and glioma cell invasion via protein kinase C and protein kinase A/NF-{kappa}B/Sp1-mediated signaling pathways Carcinogenesis, February 1, 2009; 30(2): 356 - 365. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. K. Robinson, D. P. Kelly, D. W. Mercer, and R. A. Kozar Differential Effects of Luminal Arginine and Glutamine on Metalloproteinase Production in the Postischemic Gut JPEN J Parenter Enteral Nutr, July 1, 2008; 32(4): 433 - 438. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Annabi, S. Rojas-Sutterlin, C. Laflamme, M.-P. Lachambre, Y. Rolland, H. Sartelet, and R. Beliveau Tumor Environment Dictates Medulloblastoma Cancer Stem Cell Expression and Invasive Phenotype Mol. Cancer Res., June 1, 2008; 6(6): 907 - 916. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Pottier, C. Chupin, V. Defamie, B. Cardinaud, R. Sutherland, G. Rios, F. Gauthier, P. J. Wolters, Y. Berthiaume, P. Barbry, et al. Relationships between Early Inflammatory Response to Bleomycin and Sensitivity to Lung Fibrosis: A Role for Dipeptidyl-Peptidase I and Tissue Inhibitor of Metalloproteinase-3? Am. J. Respir. Crit. Care Med., December 1, 2007; 176(11): 1098 - 1107. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-P. Han, C. Yan, L. Zhou, L. Qin, and H. Tsukamoto A Matrix Metalloproteinase-9 Activation Cascade by Hepatic Stellate Cells in Trans-differentiation in the Three-dimensional Extracellular Matrix J. Biol. Chem., April 27, 2007; 282(17): 12928 - 12939. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-M. Park, A. Kim, J.-H. Oh, and A.-S. Chung Methylseleninic acid inhibits PMA-stimulated pro-MMP-2 activation mediated by MT1-MMP expression and further tumor invasion through suppression of NF-{kappa}B activation Carcinogenesis, April 1, 2007; 28(4): 837 - 847. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Connelly, C. Robinson-Benion, M. Chont, L. Saint-Jean, H. Li, V. V. Polosukhin, T. S. Blackwell, and F. E. Yull A Transgenic Model Reveals Important Roles for the NF-{kappa}B Alternative Pathway (p100/p52) in Mammary Development and Links to Tumorigenesis J. Biol. Chem., March 30, 2007; 282(13): 10028 - 10035. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Onai, J.-i. Suzuki, Y. Maejima, G. Haraguchi, S. Muto, A. Itai, and M. Isobe Inhibition of NF-{kappa}B improves left ventricular remodeling and cardiac dysfunction after myocardial infarction Am J Physiol Heart Circ Physiol, January 1, 2007; 292(1): H530 - H538. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Sharony, G. Pintucci, P. C. Saunders, E. A. Grossi, F. G. Baumann, A. C. Galloway, and P. Mignatti Matrix metalloproteinase expression in vein grafts: role of inflammatory mediators and extracellular signal-regulated kinases-1 and -2 Am J Physiol Heart Circ Physiol, April 1, 2006; 290(4): H1651 - H1659. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Manso, L. Elsherif, S.-M. Kang, and R. S. Ross Integrins, membrane-type matrix metalloproteinases and ADAMs: Potential implications for cardiac remodeling Cardiovasc Res, February 15, 2006; 69(3): 574 - 584. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Ben-Yosef, A. Miller, S. Shapiro, and N. Lahat Hypoxia of endothelial cells leads to MMP-2-dependent survival and death Am J Physiol Cell Physiol, November 1, 2005; 289(5): C1321 - C1331. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Gerke, L. Sellin, O. Kretz, D. Petraschka, H. Zentgraf, T. Benzing, and G. Walz NEPH2 Is Located at the Glomerular Slit Diaphragm, Interacts with Nephrin and Is Cleaved from Podocytes by Metalloproteinases J. Am. Soc. Nephrol., June 1, 2005; 16(6): 1693 - 1702. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Saika, T. Miyamoto, O. Yamanaka, T. Kato, Y. Ohnishi, K. C. Flanders, K. Ikeda, Y. Nakajima, W. W.-Y. Kao, M. Sato, et al. Therapeutic Effect of Topical Administration of SN50, an Inhibitor of Nuclear Factor-{kappa}B, in Treatment of Corneal Alkali Burns in Mice Am. J. Pathol., May 1, 2005; 166(5): 1393 - 1403. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Mazzocca, S. C. Sciammetta, V. Carloni, L. Cosmi, F. Annunziato, T. Harada, S. Abrignani, and M. Pinzani Binding of Hepatitis C Virus Envelope Protein E2 to CD81 Up-regulates Matrix Metalloproteinase-2 in Human Hepatic Stellate Cells J. Biol. Chem., March 25, 2005; 280(12): 11329 - 11339. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Lobmann, G. Schultz, and H. Lehnert Proteases and the Diabetic Foot Syndrome: Mechanisms and Therapeutic Implications Diabetes Care, February 1, 2005; 28(2): 461 - 471. [Full Text] [PDF]
|
|
|
|
|
|
S. G. Park, H. Shin, Y. K. Shin, Y. Lee, E.-C. Choi, B.-J. Park, and S. Kim The Novel Cytokine p43 Stimulates Dermal Fibroblast Proliferation and Wound Repair Am. J. Pathol., February 1, 2005; 166(2): 387 - 398. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Meiners, B. Hocher, A. Weller, M. Laule, V. Stangl, C. Guenther, M. Godes, A. Mrozikiewicz, G. Baumann, and K. Stangl Downregulation of Matrix Metalloproteinases and Collagens and Suppression of Cardiac Fibrosis by Inhibition of the Proteasome Hypertension, October 1, 2004; 44(4): 471 - 477. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Xie, M. Singh, and K. Singh Differential Regulation of Matrix Metalloproteinase-2 and -9 Expression and Activity in Adult Rat Cardiac Fibroblasts in Response to Interleukin-1{beta} J. Biol. Chem., September 17, 2004; 279(38): 39513 - 39519. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Thathiah, M. Brayman, N. Dharmaraj, J. J. Julian, E. L. Lagow, and D. D. Carson Tumor Necrosis Factor {alpha} Stimulates MUC1 Synthesis and Ectodomain Release in a Human Uterine Epithelial Cell Line Endocrinology, September 1, 2004; 145(9): 4192 - 4203. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Amin, N. H. V. Chong, T. A. Bailey, J. Zhang, C. Knupp, M. E. Cheetham, J. Greenwood, and P. J. Luthert Modulation of Sub-RPE Deposits In Vitro: A Potential Model for Age-Related Macular Degeneration Invest. Ophthalmol. Vis. Sci., May 1, 2004; 45(5): 1281 - 1288. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. A. Arenas, Y. Xu, P. Lopez-Jaramillo, and S. T. Davidge Angiotensin II-induced MMP-2 release from endothelial cells is mediated by TNF-{alpha} Am J Physiol Cell Physiol, April 1, 2004; 286(4): C779 - C784. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. M. Wang, X. Zhang, D. Qian, H. Y. Lin, Q. L. Li, D. L. Liu, G. Y. Liu, X. D. Yu, and C. Zhu Effect of Ubiquitin-Proteasome Pathway on Mouse Blastocyst Implantation and Expression of Matrix Metalloproteinases-2 and -9 Biol Reprod, February 1, 2004; 70(2): 481 - 487. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Xie, M. Singh, D. A. Siwik, W. L. Joyner, and K. Singh Osteopontin Inhibits Interleukin-1{beta}-stimulated Increases in Matrix Metalloproteinase Activity in Adult Rat Cardiac Fibroblasts: ROLE OF PROTEIN KINASE C-{zeta} J. Biol. Chem., December 5, 2003; 278(49): 48546 - 48552. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Gu, D. Weihrauch, K. Tanaka, J. P. Tessmer, P. S. Pagel, J. R. Kersten, W. M. Chilian, and D. C. Warltier Reactive oxygen species are critical mediators of coronary collateral development in a canine model Am J Physiol Heart Circ Physiol, October 1, 2003; 285(4): H1582 - H1589. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B.-W. Wang, H. Chang, S. Lin, P. Kuan, and K.-G. Shyu Induction of matrix metalloproteinases-14 and -2 by cyclical mechanical stretch is mediated by tumor necrosis factor-{alpha} in cultured human umbilical vein endothelial cells Cardiovasc Res, August 1, 2003; 59(2): 460 - 469. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Liao, J. B. Thrasher, J. Pelling, J. Holzbeierlein, Q.-X. A. Sang, and B. Li Androgen Stimulates Matrix Metalloproteinase-2 Expression in Human Prostate Cancer Endocrinology, May 1, 2003; 144(5): 1656 - 1663. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Hirata, T. Sato, M. Tsumagari, A. Shimada, H. Nakano, K. Hashizume, and A. Ito Differential Regulation of the Expression of Matrix Metalloproteinases and Tissue Inhibitors of Metalloproteinases by Cytokines and Growth Factors in Bovine Endometrial Stromal Cells and Trophoblast Cell Line BT-1 In Vitro Biol Reprod, April 1, 2003; 68(4): 1276 - 1281. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Poulaki, C. S. Mitsiades, A. M. Joussen, A. Lappas, B. Kirchhof, and N. Mitsiades Constitutive Nuclear Factor-{kappa}B Activity Is Crucial for Human Retinoblastoma Cell Viability Am. J. Pathol., December 1, 2002; 161(6): 2229 - 2240. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A.-M. Khatib, L. Fallavollita, E. V. Wancewicz, B. P. Monia, and P. Brodt Inhibition of Hepatic Endothelial E-Selectin Expression by C-raf Antisense Oligonucleotides Blocks Colorectal Carcinoma Liver Metastasis Cancer Res., October 1, 2002; 62(19): 5393 - 5398. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. O. Esteve, E. Chicoine, O. Robledo, F. Aoudjit, A. Descoteaux, E. F. Potworowski, and Y. St-Pierre Protein Kinase C-zeta Regulates Transcription of the Matrix Metalloproteinase-9 Gene Induced by IL-1 and TNF-alpha in Glioma Cells via NF-kappa B J. Biol. Chem., September 13, 2002; 277(38): 35150 - 35155. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-P. Han, Y.-D. Nien, and W. L. Garner Tumor Necrosis Factor-alpha -induced Proteolytic Activation of Pro-matrix Metalloproteinase-9 by Human Skin Is Controlled by Down-regulating Tissue Inhibitor of Metalloproteinase-1 and Mediated by Tissue-associated Chymotrypsin-like Proteinase J. Biol. Chem., July 19, 2002; 277(30): 27319 - 27327. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Mitsiades, C. S. Mitsiades, V. Poulaki, D. Chauhan, P. G. Richardson, T. Hideshima, N. Munshi, S. P. Treon, and K. C. Anderson Biologic sequelae of nuclear factor-kappa B blockade in multiple myeloma: therapeutic applications Blood, May 13, 2002; 99(11): 4079 - 4086. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. A. Collins and M. D. Grounds The Role of Tumor Necrosis Factor-alpha (TNF-{alpha}) in Skeletal Muscle Regeneration: Studies in TNF-{alpha}(-/-) and TNF-{alpha}(-/-)/LT-{alpha}(-/-) Mice J. Histochem. Cytochem., August 1, 2001; 49(8): 989 - 1002. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. F. Pierce Inflammation in Nonhealing Diabetic Wounds : The Space-Time Continuum Does Matter Am. J. Pathol., August 1, 2001; 159(2): 399 - 403. [Full Text]
|
|
|
|
|
|
Y.-P. Han, T.-L. Tuan, M. Hughes, H. Wu, and W. L. Garner Transforming Growth Factor-beta - and Tumor Necrosis Factor-alpha -mediated Induction and Proteolytic Activation of MMP-9 in Human Skin J. Biol. Chem., June 15, 2001; 276(25): 22341 - 22350. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Chen, F. K. Costa, C. R. Lindvay, Y.-P. Han, and D. T. Woodley The Recombinant Expression of Full-length Type VII Collagen and Characterization of Molecular Mechanisms Underlying Dystrophic Epidermolysis Bullosa J. Biol. Chem., January 11, 2002; 277(3): 2118 - 2124. [Abstract] [Full Text] [PDF]
|
|
