|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 114, Issue 1 187-197, Copyright © 2001 by Company of Biologists
JOURNAL ARTICLES |
C Unsold, M Hyytiainen, L Bruckner-Tuderman and J Keski-Oja
Departments of Pathology and Virology, The Haartman Institute and Helsinki University Hospital, University of Helsinki, FIN-00014 Helsinki, Finland.
Latent TGF-beta binding proteins (LTBPs) are components of the extracellular matrix (ECM). They belong to the fibrillin/LTBP-superfamily, and are high molecular weight glycoproteins characterized by EGF-like repeats and 8-Cys repeats. Most LTBPs associate with the small latent forms of TGF-beta. Their roles include to facilitate the secretion of latent TGF-beta and to target it to the ECM. In order to identify new matrix-binding domains of LTBP-1 and to characterize their association with the extracellular matrix, we have produced (in a mammalian expression system) partly overlapping recombinant fragments of its shorter form, LTBP-1S, and analyzed the binding of the purified fusion proteins to extracellular matrices of cultured human dermal and lung fibroblasts. Recombinant fragments from three different regions of the N- and C-termini showed affinity to the matrix. These interacting regions contain either the first (hybrid), second or fourth 8-Cys domains of the LTBP-1S molecule. They bound independently to the matrix. Each of them had an ability to inhibit the association of native exogenous LTBP-1 with fibroblast extracellular matrix. The interactions of the LTBP-1 fragments with the extracellular matrix resisted treatment with sodium deoxycholate, suggesting strong, possibly covalent binding. The binding occurred in a time- and dose-dependent fashion. The N-terminal fragments bound more readily to the matrices. With all fragments the binding took place both with intact fibroblast matrices and with matrices isolated by sodium deoxycholate. When using CHO cell layers, which form sparse matrices, only the N-terminal fragment of LTBP-1 was efficiently incorporated. The association of the binding fragments with isolated matrices was enhanced by soluble, cell-derived factors. The current data suggest that LTBP-1 contains three different domains with an ability to associate with the extracellular matrix.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  R. N. Ono, G. Sengle, N. L. Charbonneau, V. Carlberg, H. P. Bachinger, T. Sasaki, S. Lee-Arteaga, L. Zilberberg, D. B. Rifkin, F. Ramirez, et al. Latent Transforming Growth Factor {beta}-binding Proteins and Fibulins Compete for Fibrillin-1 and Exhibit Exquisite Specificities in Binding Sites J. Biol. Chem., June 19, 2009; 284(25): 16872 - 16881. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P.-J. Wipff, D. B. Rifkin, J.-J. Meister, and B. Hinz Myofibroblast contraction activates latent TGF- 1 from the extracellular matrix J. Cell Biol., December 17, 2007; 179(6): 1311 - 1323. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Chen, P. Sivakumar, C. Barley, D. M. Peters, R. R. Gomes, M. C. Farach-Carson, and S. L. Dallas Potential Role for Heparan Sulfate Proteoglycans in Regulation of Transforming Growth Factor-beta (TGF-beta) by Modulating Assembly of Latent TGF-beta-binding Protein-1 J. Biol. Chem., September 7, 2007; 282(36): 26418 - 26430. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. M. Choe, P. H. S. Sporn, and M. A. Swartz Extracellular Matrix Remodeling by Dynamic Strain in a Three-Dimensional Tissue-Engineered Human Airway Wall Model Am. J. Respir. Cell Mol. Biol., September 1, 2006; 35(3): 306 - 313. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Fontana, Y. Chen, P. Prijatelj, T. Sakai, R. Fassler, L. Y. Sakai, and D. B. Rifkin Fibronectin is required for integrin {alpha}v{beta}6-mediated activation of latent TGF-{beta} complexes containing LTBP-1 FASEB J, November 1, 2005; 19(13): 1798 - 1808. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Mazzieri, V. Jurukovski, H. Obata, J. Sung, A. Platt, E. Annes, N. Karaman-Jurukovska, P.-E. Gleizes, and D. B. Rifkin Expression of truncated latent TGF-{beta}-binding protein modulates TGF-{beta} signaling J. Cell Sci., May 15, 2005; 118(10): 2177 - 2187. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. Dallas, P. Sivakumar, C. J. P. Jones, Q. Chen, D. M. Peters, D. F. Mosher, M. J. Humphries, and C. M. Kielty Fibronectin Regulates Latent Transforming Growth Factor-{beta} (TGF{beta}) by Controlling Matrix Assembly of Latent TGF{beta}-binding Protein-1 J. Biol. Chem., May 13, 2005; 280(19): 18871 - 18880. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. B. Rifkin Latent Transforming Growth Factor-{beta} (TGF-{beta}) Binding Proteins: Orchestrators of TGF-{beta} Availability J. Biol. Chem., March 4, 2005; 280(9): 7409 - 7412. [Full Text] [PDF]
|
|
|
|
|
|
J. P. Annes, Y. Chen, J. S. Munger, and D. B. Rifkin Integrin {alpha}V{beta}6-mediated activation of latent TGF-{beta} requires the latent TGF-{beta} binding protein-1 J. Cell Biol., June 7, 2004; 165(5): 723 - 734. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Hyytiainen and J. Keski-Oja Latent TGF-{beta} binding protein LTBP-2 decreases fibroblast adhesion to fibronectin J. Cell Biol., December 22, 2003; 163(6): 1363 - 1374. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Vehvilainen, M. Hyytiainen, and J. Keski-Oja Latent Transforming Growth Factor-{beta}-binding Protein 2 Is an Adhesion Protein for Melanoma Cells J. Biol. Chem., June 27, 2003; 278(27): 24705 - 24713. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Kanzaki and M. Otabe Latent Transforming Growth Factor-{beta} Binding Protein-1, a Component of Latent Transforming Growth Factor-{beta} Complex, Accelerates the Migration of Aortic Smooth Muscle Cells in Diabetic Rats Through Integrin-{beta}3 Diabetes, March 1, 2003; 52(3): 824 - 828. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Isogai, R. N. Ono, S. Ushiro, D. R. Keene, Y. Chen, R. Mazzieri, N. L. Charbonneau, D. P. Reinhardt, D. B. Rifkin, and L. Y. Sakai Latent Transforming Growth Factor beta -binding Protein 1 Interacts with Fibrillin and Is a Microfibril-associated Protein J. Biol. Chem., January 17, 2003; 278(4): 2750 - 2757. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. P. Annes, J. S. Munger, and D. B Rifkin Making sense of latent TGF{beta} activation J. Cell Sci., January 15, 2003; 116(2): 217 - 224. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Penttinen, J. Saharinen, K. Weikkolainen, M. Hyytiainen, and J. Keski-Oja Secretion of human latent TGF-{beta}-binding protein-3 (LTBP-3) is dependent on co-expression of TGF-{beta} J. Cell Sci., January 9, 2002; 115(17): 3457 - 3468. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Koli, J. Saharinen, M. Karkkainen, and J. Keski-Oja Novel non-TGF-{beta}-binding splice variant of LTBP-4 in human cells and tissues provides means to decrease TGF-{beta} deposition J. Cell Sci., January 8, 2001; 114(15): 2869 - 2878. [Abstract] [Full Text] [PDF]
|
|
