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Journal of Cell Science, Vol 114, Issue 10 1839-1845, Copyright © 2001 by Company of Biologists

JOURNAL ARTICLES

Transcriptional activation by the human Hsp70-associating protein Hap50

Y Niyaz, M Zeiner and U Gehring
Ruprecht-Karls-Universitat Heidelberg, Biochemie-Zentrum Heidelberg, Biologische Chemie, Im Neuenheimer Feld 501, D-69120 Heidelberg, Germany. ugehring@sun0.urz.uni-heidelberg.de

We investigated human Hap50, the large isoform of the previously characterized Hsp70/Hsc70-associating protein Hap46, also called BAG-1, for effects on transcriptional activities. Overproduction by transient transfection led to enhanced expression of reporter gene constructs in various cell types using different promoters, suggesting independence of promoter type. Similarly, overexpression of Hap50 resulted in increased levels of poly(A)(+ )mRNAs in HeLa, COS-7, 3T3 and HTC cells. Concomitantly, the expression of some selected endogenous genes, such as those coding for c-Jun and the glucocorticoid receptor, was enhanced significantly relative to actin. Nuclear runoff transcription assays using HeLa cells showed that the effect is caused by increased transcription rates rather than mRNA stabilization. Activation of transcription by Hap50 occurred at 37 degrees C and did not require prior thermal stress, as is the case for Hap46. In accordance with these biological effects, Hap50 is localized exclusively in the nuclear compartment of different cell types, whereas Hap46 is mostly cytoplasmic in unstressed cells, as revealed by use of fusion constructs with green fluorescent protein. High cellular levels of Hap50 were found to make cells less susceptible to adverse environmental effects such as heat stress. Our data suggest that Hap50 is a nuclear protein that acts in cells to increase the transcription of various genes.


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© The Company of Biologists Ltd 2001