spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zahn, R.
Right arrow Articles by Munn, A. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zahn, R.
Right arrow Articles by Munn, A. L.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Journal of Cell Science, Vol 114, Issue 10 1935-1947, Copyright © 2001 by Company of Biologists

JOURNAL ARTICLES

End13p/Vps4p is required for efficient transport from early to late endosomes in Saccharomyces cerevisiae

R Zahn, BJ Stevenson, S Schroder-Kohne, B Zanolari, H Riezman and AL Munn
Institute of Molecular Agrobiology, National University of Singapore, Singapore, Republic of Singapore. alan@ima.org.sg

end13-1 was isolated in a screen for endocytosis mutants and has been shown to have a post-internalisation defect in endocytic transport as well as a defect in vacuolar protein sorting (Vps(-) phenotype), leading to secretion of newly synthesised vacuolar proteins. Here we demonstrate that END13 is identical to VPS4, encoding an AAA (ATPase associated with a variety of cellular activities)-family ATPase. We also report that the end13-1 mutation is a serine 335 to phenylalanine substitution in the AAA-ATPase domain of End13p/Vps4p. It has been reported that mutant cells lacking End13p/Vps4p (end13(vps4)((Dgr;)) accumulate endocytosed marker dyes, plasma membrane receptors and newly synthesised vacuolar hydrolase precursors in an endosomal compartment adjacent to the vacuole (prevacuolar compartment, or PVC). We find, however, that the end13 mutants have defects in transport of endocytosed fluorescent dyes, plasma membrane receptors and ligands from small peripherally located early endosomes to larger late endosomes, which are often located adjacent to the vacuole. Our results indicate that End13p/Vps4p may play an important role in multiple steps of membrane traffic through the endocytic pathway.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
A. C. Berger, G. Salazar, M. L. Styers, K. A. Newell-Litwa, E. Werner, R. A. Maue, A. H. Corbett, and V. Faundez
The subcellular localization of the Niemann-Pick Type C proteins depends on the adaptor complex AP-3
J. Cell Sci., October 15, 2007; 120(20): 3640 - 3652.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
J. Huang, F. Reggiori, and D. J. Klionsky
The Transmembrane Domain of Acid Trehalase Mediates Ubiquitin-independent Multivesicular Body Pathway Sorting
Mol. Biol. Cell, July 1, 2007; 18(7): 2511 - 2524.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Nebauer, S. Rosenberger, and G. Daum
Phosphatidylethanolamine, a Limiting Factor of Autophagy in Yeast Strains Bearing a Defect in the Carboxypeptidase Y Pathway of Vacuolar Targeting
J. Biol. Chem., June 8, 2007; 282(23): 16736 - 16743.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
G. Gabriely, R. Kama, and J. E. Gerst
Involvement of Specific COPI Subunits in Protein Sorting from the Late Endosome to the Vacuole in Yeast
Mol. Cell. Biol., January 15, 2007; 27(2): 526 - 540.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
O. Staub and D. Rotin
Role of Ubiquitylation in Cellular Membrane Transport
Physiol Rev, April 1, 2006; 86(2): 669 - 707.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. Doyotte, M. R. G. Russell, C. R. Hopkins, and P. G. Woodman
Depletion of TSG101 forms a mammalian `Class E' compartment: a multicisternal early endosome with multiple sorting defects
J. Cell Sci., July 15, 2005; 118(14): 3003 - 3017.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
S. C. L. Yeo, L. Xu, J. Ren, V. J. Boulton, M. D. Wagle, C. Liu, G. Ren, P. Wong, R. Zahn, P. Sasajala, et al.
Vps20p and Vta1p interact with Vps4p and function in multivesicular body sorting and endosomal transport in Saccharomyces cerevisiae
J. Cell Sci., October 1, 2003; 116(19): 3957 - 3970.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
H. Fujita, M. Yamanaka, K. Imamura, Y. Tanaka, A. Nara, T. Yoshimori, S. Yokota, and M. Himeno
A dominant negative form of the AAA ATPase SKD1/VPS4 impairs membrane trafficking out of endosomal/lysosomal compartments: class E vps phenotype in mammalian cells
J. Cell Sci., January 15, 2003; 116(2): 401 - 414.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2001