spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Voorn-Brouwer, T.
Right arrow Articles by Distel, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Voorn-Brouwer, T.
Right arrow Articles by Distel, B.
Journal of Cell Science 114, 2199-2204 (2001)
© 2001 The Company of Biologists Limited

RESEARCH ARTICLE

Peroxisomal membrane proteins are properly targeted to peroxisomes in the absence of COPI- and COPII-mediated vesicular transport

Tineke Voorn-Brouwer, Astrid Kragt, Henk F. Tabak and Ben Distel*

Department of Biochemistry, University of Amsterdam, Academic Medical Center, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands

*Author for correspondence (e-mail: b.distel{at}amc.uva.nl)

Accepted March 2, 2001

The classic model for peroxisome biogenesis states that new peroxisomes arise by the fission of pre-existing ones and that peroxisomal matrix and membrane proteins are recruited directly from the cytosol. Recent studies challenge this model and suggest that some peroxisomal membrane proteins might traffic via the endoplasmic reticulum to peroxisomes. We have studied the trafficking in human fibroblasts of three peroxisomal membrane proteins, Pex2p, Pex3p and Pex16p, all of which have been suggested to transit the endoplasmic reticulum before arriving in peroxisomes. Here, we show that targeting of these peroxisomal membrane proteins is not affected by inhibitors of COPI and COPII that block vesicle transport in the early secretory pathway. Moreover, we have obtained no evidence for the presence of these peroxisomal membrane proteins in compartments other than peroxisomes and demonstrate that COPI and COPII inhibitors do not affect peroxisome morphology or integrity. Together, these data fail to provide any evidence for a role of the endoplasmic reticulum in peroxisome biogenesis.

Key words: Membrane biogenesis, Protein targeting, Vesicular transport, Peroxisome biogenesis, Coatomer




This article has been cited by other articles:


Home page
 J. Cell Biol.Home page
V. I. Titorenko and R. T. Mullen
Peroxisome biogenesis: the peroxisomal endomembrane system and the role of the ER
J. Cell Biol., July 3, 2006; 174(1): 11 - 17.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. van der Zand, I. Braakman, H. J. Geuze, and H. F. Tabak
The return of the peroxisome.
J. Cell Sci., March 15, 2006; 119(Pt 6): 989 - 994.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Y. C. Tam, A. Fagarasanu, M. Fagarasanu, and R. A. Rachubinski
Pex3p Initiates the Formation of a Preperoxisomal Compartment from a Subdomain of the Endoplasmic Reticulum in Saccharomyces cerevisiae
J. Biol. Chem., October 14, 2005; 280(41): 34933 - 34939.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Kragt, T. Voorn-Brouwer, M. van den Berg, and B. Distel
Endoplasmic Reticulum-directed Pex3p Routes to Peroxisomes and Restores Peroxisome Formation in a Saccharomyces cerevisiae pex3{Delta} Strain
J. Biol. Chem., October 7, 2005; 280(40): 34350 - 34357.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
I. A. Sparkes, C. Hawes, and A. Baker
AtPEX2 and AtPEX10 Are Targeted to Peroxisomes Independently of Known Endoplasmic Reticulum Trafficking Routes
Plant Physiology, October 1, 2005; 139(2): 690 - 700.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
M. Marelli, J. J. Smith, S. Jung, E. Yi, A. I. Nesvizhskii, R. H. Christmas, R. A. Saleem, Y. Y. C. Tam, A. Fagarasanu, D. R. Goodlett, et al.
Quantitative mass spectrometry reveals a role for the GTPase Rho1p in actin organization on the peroxisome membrane
J. Cell Biol., December 20, 2004; 167(6): 1099 - 1112.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. A. Murphy, B. A. Phillipson, A. Baker, and R. T. Mullen
Characterization of the Targeting Signal of the Arabidopsis 22-kD Integral Peroxisomal Membrane Protein
Plant Physiology, October 1, 2003; 133(2): 813 - 828.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
H. J. Geuze, J. L. Murk, A. K. Stroobants, J. M. Griffith, M. J. Kleijmeer, A. J. Koster, A. J. Verkleij, B. Distel, and H. F. Tabak
Involvement of the Endoplasmic Reticulum in Peroxisome Formation
Mol. Biol. Cell, July 1, 2003; 14(7): 2900 - 2907.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
X. Li, E. Baumgart, G.-X. Dong, J. C. Morrell, G. Jimenez-Sanchez, D. Valle, K. D. Smith, and S. J. Gould
PEX11{alpha} Is Required for Peroxisome Proliferation in Response to 4-Phenylbutyrate but Is Dispensable for Peroxisome Proliferator-Activated Receptor Alpha-Mediated Peroxisome Proliferation
Mol. Cell. Biol., December 1, 2002; 22(23): 8226 - 8240.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Honsho, T. Hiroshige, and Y. Fujiki
The Membrane Biogenesis Peroxin Pex16p. TOPOGENESIS AND FUNCTIONAL ROLES IN PEROXISOMAL MEMBRANE ASSEMBLY
J. Biol. Chem., November 8, 2002; 277(46): 44513 - 44524.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2001