|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
RESEARCH ARTICLE |
Vollum Institute, L474, Oregon Health Sciences University, 3181 S.W. Sam Jackson Park Rd, Portland, OR 97201-3098, USA
*Author for correspondence (e-mail: stauffer{at}ohsu.edu)
Accepted January 18, 2001
A multivesicular body is a vesicle-filled endosome that targets proteins to the interior of lysosomes. We have identified a conserved eukaryotic protein, human CHMP1, which is strongly implicated in multivesicular body formation. Immunocytochemistry and biochemical fractionation localize CHMP1 to early endosomes and CHMP1 physically interacts with SKD1/VPS4, a highly conserved protein directly linked to multivesicular body sorting in yeast. Similar to the action of a mutant SKD1 protein, overexpression of a fusion derivative of human CHMP1 dilates endosomal compartments and disrupts the normal distribution of several endosomal markers. Genetic studies in Saccharomyces cerevisiae further support a conserved role of CHMP1 in vesicle trafficking. Deletion of CHM1, the budding yeast homolog of CHMP1, results in defective sorting of carboxypeptidases S and Y and produces abnormal, multi-lamellar prevacuolar compartments. This phenotype classifies CHM1 as a member of the class E vacuolar protein sorting genes. Yeast Chm1p belongs to a structurally-related, but rather divergent family of proteins, including Vps24p and Snf7p and three novel proteins, Chm2p, Chm5p and Chm6p, which are all essential for multivesicular body sorting. These observations identify the conserved CHMP/Chmp family as a set of proteins fundamental to understanding multivesicular body sorting in eukaryotic organisms.
Key words: Biological transport, Endosomes, Vacuoles, Carboxypeptidases, Nuclear matrix, Gene silencing
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  T. Pawliczek and C. M. Crump Herpes Simplex Virus Type 1 Production Requires a Functional ESCRT-III Complex but Is Independent of TSG101 and ALIX Expression J. Virol., November 1, 2009; 83(21): 11254 - 11264. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Tandon, D. P. AuCoin, and E. S. Mocarski Human Cytomegalovirus Exploits ESCRT Machinery in the Process of Virion Maturation J. Virol., October 15, 2009; 83(20): 10797 - 10807. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. McDonald and J. Martin-Serrano No strings attached: the ESCRT machinery in viral budding and cytokinesis J. Cell Sci., July 1, 2009; 122(13): 2167 - 2177. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Bajorek, E. Morita, J. J. Skalicky, S. G. Morham, M. Babst, and W. I. Sundquist Biochemical Analyses of Human IST1 and Its Function in Cytokinesis Mol. Biol. Cell, March 1, 2009; 20(5): 1360 - 1373. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Rodriguez-Galan, A. Galindo, A. Hervas-Aguilar, H. N. Arst Jr., and M. A. Penalva Physiological Involvement in pH Signaling of Vps24-mediated Recruitment of Aspergillus PalB Cysteine Protease to ESCRT-III J. Biol. Chem., February 13, 2009; 284(7): 4404 - 4412. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Weiss, S. Huppert, and R. Kolling ESCRT-III Protein Snf7 Mediates High-Level Expression of the SUC2 Gene via the Rim101 Pathway Eukaryot. Cell, November 1, 2008; 7(11): 1888 - 1894. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R G E van Eijsden, L M T Eijssen, P J Lindsey, C M M van den Burg, L E A de Wit, M E Rubio-Gozalbo, C E M de Die, T Ayoubi, W Sluiter, I F M de Coo, et al. Termination of damaged protein repair defines the occurrence of symptoms in carriers of the m.3243A>G tRNALeu mutation J. Med. Genet., August 1, 2008; 45(8): 525 - 534. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Yorikawa, E. Takaya, Y. Osako, R. Tanaka, Y. Terasawa, T. Hamakubo, Y. Mochizuki, H. Iwanari, T. Kodama, T. Maeda, et al. Human Calpain 7/PalBH Associates with a Subset of ESCRT-III-related Proteins in its N-terminal Region and Partly Localizes to Endocytic Membrane Compartments J. Biochem., June 1, 2008; 143(6): 731 - 745. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Shim, S. A. Merrill, and P. I. Hanson Novel Interactions of ESCRT-III with LIP5 and VPS4 and their Implications for ESCRT-III Disassembly Mol. Biol. Cell, June 1, 2008; 19(6): 2661 - 2672. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Dimaano, C. B. Jones, A. Hanono, M. Curtiss, and M. Babst Ist1 Regulates Vps4 Localization and Assembly Mol. Biol. Cell, February 1, 2008; 19(2): 465 - 474. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. E. Row, H. Liu, S. Hayes, R. Welchman, P. Charalabous, K. Hofmann, M. J. Clague, C. M. Sanderson, and S. Urbe The MIT Domain of UBPY Constitutes a CHMP Binding and Endosomal Localization Signal Required for Efficient Epidermal Growth Factor Receptor Degradation J. Biol. Chem., October 19, 2007; 282(42): 30929 - 30937. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Tian, L. Olsen, B. Sun, S. E. Lid, R. C. Brown, B. E. Lemmon, K. Fosnes, D. Gruis, H.-G. Opsahl-Sorteberg, M. S. Otegui, et al. Subcellular Localization and Functional Domain Studies of DEFECTIVE KERNEL1 in Maize and Arabidopsis Suggest a Model for Aleurone Cell Fate Specification Involving CRINKLY4 and SUPERNUMERARY ALEURONE LAYER1 PLANT CELL, October 1, 2007; 19(10): 3127 - 3145. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Usami, S. Popov, and H. G. Gottlinger Potent Rescue of Human Immunodeficiency Virus Type 1 Late Domain Mutants by ALIX/AIP1 Depends on Its CHMP4 Binding Site J. Virol., June 15, 2007; 81(12): 6614 - 6622. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Irie, Y. Shimazu, T. Yoshida, and T. Sakaguchi The YLDL Sequence within Sendai Virus M Protein Is Critical for Budding of Virus-Like Particles and Interacts with Alix/AIP1 Independently of C Protein J. Virol., March 1, 2007; 81(5): 2263 - 2273. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Zamborlini, Y. Usami, S. R. Radoshitzky, E. Popova, G. Palu, and H. Gottlinger Release of autoinhibition converts ESCRT-III components into potent inhibitors of HIV-1 budding PNAS, December 12, 2006; 103(50): 19140 - 19145. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. P. Nickerson, M. West, and G. Odorizzi Did2 coordinates Vps4-mediated dissociation of ESCRT-III from endosomes J. Cell Biol., November 27, 2006; (2006) jcb.200606113. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Scott, J. Gaspar, M. D. Stuchell-Brereton, S. L. Alam, J. J. Skalicky, and W. I. Sundquist Structure and ESCRT-III protein interactions of the MIT domain of human VPS4A PNAS, September 27, 2005; 102(39): 13813 - 13818. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. L. Robinson and J. E. Dixon The Phosphoinositide-3-phosphatase MTMR2 Associates with MTMR13, a Membrane-associated Pseudophosphatase Also Mutated in Type 4B Charcot-Marie-Tooth Disease J. Biol. Chem., September 9, 2005; 280(36): 31699 - 31707. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Lin, L. A. Kimpler, T. V. Naismith, J. M. Lauer, and P. I. Hanson Interaction of the Mammalian Endosomal Sorting Complex Required for Transport (ESCRT) III Protein hSnf7-1 with Itself, Membranes, and the AAA+ ATPase SKD1 J. Biol. Chem., April 1, 2005; 280(13): 12799 - 12809. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Ward, M. B. Vaughn, S. L. Shiflett, P. L. White, A. L. Pollock, J. Hill, R. Schnegelberger, W. I. Sundquist, and J. Kaplan The Role of LIP5 and CHMP5 in Multivesicular Body Formation and HIV-1 Budding in Mammalian Cells J. Biol. Chem., March 18, 2005; 280(11): 10548 - 10555. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Reid, J. Connell, T. L. Edwards, S. Duley, S. E. Brown, and C. M. Sanderson The hereditary spastic paraplegia protein spastin interacts with the ESCRT-III complex-associated endosomal protein CHMP1B Hum. Mol. Genet., January 1, 2005; 14(1): 19 - 38. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Pisitkun, R.-F. Shen, and M. A. Knepper Identification and proteomic profiling of exosomes in human urine PNAS, September 7, 2004; 101(36): 13368 - 13373. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Fujita, Y. Umezuki, K. Imamura, D. Ishikawa, S. Uchimura, A. Nara, T. Yoshimori, Y. Hayashizaki, J. Kawai, K. Ishidoh, et al. Mammalian class E Vps proteins, SBP1 and mVps2/CHMP2A, interact with and regulate the function of an AAA-ATPase SKD1/Vps4B J. Cell Sci., June 15, 2004; 117(14): 2997 - 3009. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Martin-Serrano, A. Yarovoy, D. Perez-Caballero, and P. D. Bieniasz Divergent retroviral late-budding domains recruit vacuolar protein sorting factors by using alternative adaptor proteins PNAS, October 14, 2003; 100(21): 12414 - 12419. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Whitley, B. J. Reaves, M. Hashimoto, A. M. Riley, B. V. L. Potter, and G. D. Holman Identification of Mammalian Vps24p as an Effector of Phosphatidylinositol 3,5-Bisphosphate-dependent Endosome Compartmentalization J. Biol. Chem., October 3, 2003; 278(40): 38786 - 38795. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Katoh, H. Shibata, H. Suzuki, A. Nara, K. Ishidoh, E. Kominami, T. Yoshimori, and M. Maki The ALG-2-interacting Protein Alix Associates with CHMP4b, a Human Homologue of Yeast Snf7 That Is Involved in Multivesicular Body Sorting J. Biol. Chem., October 3, 2003; 278(40): 39104 - 39113. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. L. Yeo, L. Xu, J. Ren, V. J. Boulton, M. D. Wagle, C. Liu, G. Ren, P. Wong, R. Zahn, P. Sasajala, et al. Vps20p and Vta1p interact with Vps4p and function in multivesicular body sorting and endosomal transport in Saccharomyces cerevisiae J. Cell Sci., October 1, 2003; 116(19): 3957 - 3970. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Shen, C. Li, Z. Min, R. B. Meeley, M. C. Tarczynski, and O.-A. Olsen sal1 determines the number of aleurone cell layers in maize endosperm and encodes a class E vacuolar sorting protein PNAS, May 27, 2003; 100(11): 6552 - 6557. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Fujita, M. Yamanaka, K. Imamura, Y. Tanaka, A. Nara, T. Yoshimori, S. Yokota, and M. Himeno A dominant negative form of the AAA ATPase SKD1/VPS4 impairs membrane trafficking out of endosomal/lysosomal compartments: class E vps phenotype in mammalian cells J. Cell Sci., January 15, 2003; 116(2): 401 - 414. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. R. Stauffer, T. L. Howard, T. Nyun, and S. M. Hollenberg CHMP1 is a novel nuclear matrix protein affecting chromatin structure and cell-cycle progression J. Cell Sci., January 7, 2001; 114(13): 2383 - 2393. [Abstract] [Full Text] [PDF]
|
|
