spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rupper, A.
Right arrow Articles by Cardelli, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rupper, A.
Right arrow Articles by Cardelli, J.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?
Journal of Cell Science 114, 2449-2460 (2001)
© 2001 The Company of Biologists Limited

RESEARCH ARTICLE

Rab7 regulates phagosome maturation in Dictyostelium

Adam Rupper1, Bryon Grove2 and James Cardelli1,*

1 Department of Microbiology and Immunology and The Feist/Weiller Cancer Center, LSUHSC, Shreveport, LA 71130, USA
2 Department of Anatomy and Cell Biology, University of North Dakota, Grand Forks, North Dakota 58202, USA

*Author for correspondence (e-mail: jcarde{at}lsuhsc.edu)

Accepted April 6, 2001

A Dictyostelium Rab7 homolog has been demonstrated to regulate fluid-phase influx, efflux, retention of lysosomal hydrolases and phagocytosis. Since Rab7 function appeared to be required for efficient phagocytosis, we sought to further characterize the role of Rab7 in phagosomal maturation. Expression of GFP-Rab7 resulted in labeling of both early and late phagosomes containing yeast, but not forming phagocytic cups. In order to determine if Rab7 played a role in regulating membrane traffic between the endo/lysosomal system and maturing phagosomes, latex bead containing (LBC) phagosomes were purified from wild-type cells at various times after internalization. Glycosidases, cysteine proteinases, Rab7 and lysosomally associated membrane proteins were delivered rapidly to nascent phagosomes in control cells. LBC phagosomes isolated from cells overexpressing dominant negative (DN) Rab7 contained very low levels of LmpA (lysosomal integral membrane protein) and {alpha}-mannosidase was not detectable. Interestingly, cysteine proteinases were delivered to phagosomes as apparent pro-forms in cells overexpressing DN Rab7. Despite these defects, phagosomes in cells overexpressing DN Rab7 matured to form multi-particle spacious phagosomes, except that these phagosomes remained significantly more acidic than control phagosomes. These results suggested that Rab7 regulates both an early and late steps of phagosomal maturation, similar to its role in the endo/lysosomal system.

Key words: Rab7, Phagocytosis, Phagosome maturation


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. ProteomicsHome page
I. Jutras, M. Houde, N. Currier, J. Boulais, S. Duclos, S. LaBoissiere, E. Bonneil, P. Kearney, P. Thibault, E. Paramithiotis, et al.
Modulation of the Phagosome Proteome by Interferon-{gamma}
Mol. Cell. Proteomics, April 1, 2008; 7(4): 697 - 715.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
J. Fang, J. A. Brzostowski, S. Ou, N. Isik, V. Nair, and T. Jin
A vesicle surface tyrosine kinase regulates phagosome maturation
J. Cell Biol., July 24, 2007; 178(3): 411 - 423.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
S. Saxena, C. Bucci, J. Weis, and A. Kruttgen
The Small GTPase Rab7 Controls the Endosomal Trafficking and Neuritogenic Signaling of the Nerve Growth Factor Receptor TrkA
J. Neurosci., November 23, 2005; 25(47): 10930 - 10940.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Saito-Nakano, T. Yasuda, K. Nakada-Tsukui, M. Leippe, and T. Nozaki
Rab5-associated Vacuoles Play a Unique Role in Phagocytosis of the Enteric Protozoan Parasite Entamoeba histolytica
J. Biol. Chem., November 19, 2004; 279(47): 49497 - 49507.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
R. E. Harrison, C. Bucci, O. V. Vieira, T. A. Schroer, and S. Grinstein
Phagosomes Fuse with Late Endosomes and/or Lysosomes by Extension of Membrane Protrusions along Microtubules: Role of Rab7 and RILP
Mol. Cell. Biol., September 15, 2003; 23(18): 6494 - 6506.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
E. Garcia-Garcia and C. Rosales
Signal transduction during Fc receptor-mediated phagocytosis
J. Leukoc. Biol., December 1, 2002; 72(6): 1092 - 1108.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
E. Harris and J. Cardelli
RabD, a Dictyostelium Rab14-related GTPase, regulates phagocytosis and homotypic phagosome and lysosome fusion
J. Cell Sci., September 15, 2002; 115(18): 3703 - 3713.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
M. Clarke, J. Kohler, Q. Arana, T. Liu, J. Heuser, and G. Gerisch
Dynamics of the vacuolar H+-ATPase in the contractile vacuole complex and the endosomal pathway of Dictyostelium cells
J. Cell Sci., July 15, 2002; 115(14): 2893 - 2905.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2001