QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

This Article Figures Only Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Janssens, B. Articles by van Roy, F. Search for Related Content PubMed PubMed Citation Articles by Janssens, B. Articles by van Roy, F.

T-Catenin: a novel tissue-specific ß-catenin-binding protein mediating strong cell-cell adhesion

¹ Molecular Cell Biology Unit, Department of Molecular Biology, Flanders Interuniversity Institute for Biotechnology (VIB)-Ghent University, B-9000 Ghent, Belgium
² Department of Pathology, University Hospital Antwerp (UZA), B-2650 Edegem, Belgium
³ Department of Radiotherapy, Nuclear Medicine and Experimental Cancerology, University Hospital Ghent, B-9000 Ghent, Belgium

*Author for correspondence (e-mail: f.vanroy{at}dmb.rug.ac.be)

Accepted May 28, 2001

Cadherins are major cell-cell adhesion proteins whose cytoplasmic domains bind to catenin proteins. Strong intercellular adhesion depends on linkage of the cadherin/catenin complex to the actin cytoskeleton via -catenin. To date, it is not clear how different cell types achieve the variable strength of cell-cell adhesion clearly needed in a multicellular organism. Here, we report the cloning and molecular characterization of T(testis)-catenin, a novel human cDNA encoding a protein with homology to both human E(epithelial)-catenin and N(neural)-catenin. Although originally discovered in testis, T-catenin is expressed in other tissues, the highest levels being observed in heart. Immunohistochemical analysis showed human T-catenin localization at intercalated discs of cardiomyocytes and in peritubular myoid cells of testis. In cells transfected with T-catenin cDNA, interaction with ß-catenin was demonstrated by co-immunoprecipitation. Transfection of -catenin-deficient colon carcinoma cells recruited E-cadherin and ß-catenin to cell-cell contacts and functional cadherin-mediated cell-cell adhesion was restored in this way. Moreover, compaction of these cells was at least as prominent as in the case of cells expressing endogenous E-catenin. We propose that T-catenin is necessary for the formation of stretch-resistant cell-cell adhesion complexes, in particular, muscle cells.

Key words: Catenin, Cadherin, Muscle, Heart, Testis

This article has been cited by other articles:

				D. D. Mruk, B. Silvestrini, and C. Y. Cheng Anchoring Junctions As Drug Targets: Role in Contraceptive Development Pharmacol. Rev., June 1, 2008; 60(2): 146 - 180. [Abstract] [Full Text] [PDF]

				A. Miyashita, H. Arai, T. Asada, M. Imagawa, E. Matsubara, M. Shoji, S. Higuchi, K. Urakami, A. Kakita, H. Takahashi, et al. Genetic association of CTNNA3 with late-onset Alzheimer's disease in females Hum. Mol. Genet., December 1, 2007; 16(23): 2854 - 2869. [Abstract] [Full Text] [PDF]

				S. Goossens, B. Janssens, S. Bonne, R. De Rycke, F. Braet, J. van Hengel, and F. van Roy A unique and specific interaction between {alpha}T-catenin and plakophilin-2 in the area composita, the mixed-type junctional structure of cardiac intercalated discs J. Cell Sci., June 15, 2007; 120(12): 2126 - 2136. [Abstract] [Full Text] [PDF]

				S. Goossens, B. Janssens, G. Vanpoucke, R. De Rycke, J. van Hengel, and F. van Roy Truncated isoform of mouse {alpha}T-catenin is testis-restricted in expression and function FASEB J, March 1, 2007; 21(3): 647 - 655. [Abstract] [Full Text] [PDF]

				L. A. Meza-Zepeda, S. H. Kresse, A. H. Barragan-Polania, B. Bjerkehagen, H. O. Ohnstad, H. M. Namlos, J. Wang, B. E. Kristiansen, and O. Myklebost Array Comparative Genomic Hybridization Reveals Distinct DNA Copy Number Differences between Gastrointestinal Stromal Tumors and Leiomyosarcomas. Cancer Res., September 15, 2006; 66(18): 8984 - 8993. [Abstract] [Full Text] [PDF]

				F. Sheikh, Y. Chen, X. Liang, A. Hirschy, A. E. Stenbit, Y. Gu, N. D. Dalton, T. Yajima, Y. Lu, K. U. Knowlton, et al. {alpha}-E-Catenin Inactivation Disrupts the Cardiomyocyte Adherens Junction, Resulting in Cardiomyopathy and Susceptibility to Wall Rupture Circulation, September 5, 2006; 114(10): 1046 - 1055. [Abstract] [Full Text] [PDF]

				A. Soubry, J. van Hengel, E. Parthoens, C. Colpaert, E. Van Marck, D. Waltregny, A. B. Reynolds, and F. van Roy Expression and Nuclear Location of the Transcriptional Repressor Kaiso Is Regulated by the Tumor Microenvironment Cancer Res., March 15, 2005; 65(6): 2224 - 2233. [Abstract] [Full Text] [PDF]

				K. S. Grossmann, C. Grund, J. Huelsken, M. Behrend, B. Erdmann, W. W. Franke, and W. Birchmeier Requirement of plakophilin 2 for heart morphogenesis and cardiac junction formation J. Cell Biol., October 11, 2004; 167(1): 149 - 160. [Abstract] [Full Text] [PDF]

				G. Vanpoucke, S. Goossens, B. De Craene, B. Gilbert, F. van Roy, and G. Berx GATA-4 and MEF2C transcription factors control the tissue-specific expression of the {alpha}T-catenin gene CTNNA3 Nucleic Acids Res., August 9, 2004; 32(14): 4155 - 4165. [Abstract] [Full Text] [PDF]

				C. B.M. Oudejans, J. Mulders, A. M.A. Lachmeijer, M. van Dijk, A. A.M. Konst, B. A. Westerman, I. J. van Wijk, P. A.J. Leegwater, H. D. Kato, T. Matsuda, et al. The parent-of-origin effect of 10q22 in pre-eclamptic females coincides with two regions clustered for genes with down-regulated expression in androgenetic placentas Mol. Hum. Reprod., August 1, 2004; 10(8): 589 - 598. [Abstract] [Full Text] [PDF]

				N. P.Y. Lee and C. Y. Cheng Ectoplasmic specialization, a testis-specific cell-cell actin-based adherens junction type: is this a potential target for male contraceptive development? Hum. Reprod. Update, July 1, 2004; 10(4): 349 - 369. [Abstract] [Full Text] [PDF]

				N. Ertekin-Taner, J. Ronald, H. Asahara, L. Younkin, M. Hella, S. Jain, E. Gnida, S. Younkin, D. Fadale, Y. Ohyagi, et al. Fine mapping of the {alpha}-T catenin gene to a quantitative trait locus on chromosome 10 in late-onset Alzheimer's disease pedigrees Hum. Mol. Genet., December 1, 2003; 12(23): 3133 - 3143. [Abstract] [Full Text] [PDF]

				X. Guo, J. N. Rao, L. Liu, T.-T. Zou, D. J. Turner, B. L. Bass, and J.-Y. Wang Regulation of adherens junctions and epithelial paracellular permeability: a novel function for polyamines Am J Physiol Cell Physiol, November 1, 2003; 285(5): C1174 - C1187. [Abstract] [Full Text] [PDF]

				Y. Luo and G. L. Radice Cadherin-mediated adhesion is essential for myofibril continuity across the plasma membrane but not for assembly of the contractile apparatus J. Cell Sci., April 15, 2003; 116(8): 1471 - 1479. [Abstract] [Full Text] [PDF]

				M. Mareel and A. Leroy Clinical, Cellular, and Molecular Aspects of Cancer Invasion Physiol Rev, April 1, 2003; 83(2): 337 - 376. [Abstract] [Full Text] [PDF]

				W.-Y. Lui, D. D. Mruk, W. M. Lee, and C. Y. Cheng Adherens Junction Dynamics in the Testis and Spermatogenesis J Androl, January 1, 2003; 24(1): 1 - 14. [Full Text] [PDF]

				M. C. Ferreira-Cornwell, Y. Luo, N. Narula, J. M. Lenox, M. Lieberman, and G. L. Radice Remodeling the intercalated disc leads to cardiomyopathy in mice misexpressing cadherins in the heart J. Cell Sci., April 15, 2002; 115(8): 1623 - 1634. [Abstract] [Full Text] [PDF]

				K. J. Johnson and K. Boekelheide Dynamic Testicular Adhesion Junctions Are Immunologically Unique. II. Localization of Classic Cadherins in Rat Testis Biol Reprod, April 1, 2002; 66(4): 992 - 1000. [Abstract] [Full Text] [PDF]