|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
RESEARCH ARTICLE |
1 Department of Biochemistry and Cell Biology, Utrecht University, Utrecht, 3584 CM, The Netherlands
2 Department of Farm Animal Health, Graduate School of Animal Health, Utrecht University, Utrecht, 3584 CL, The Netherlands
3 Department of Molecular Cell Biology, Institute of Biomembranes, Utrecht University, Utrecht, 3584 CH, The Netherlands
*Author for correspondence (e-mail: b.gadella{at}vet.uu.nl)
Accepted June 24, 2001
Mammalian sperm cells are activated prior to fertilization by high bicarbonate levels, which facilitate lipoprotein-mediated cholesterol efflux. The role of bicarbonate and cholesterol acceptors on the cholesterol organization in the sperm plasma membrane was tested. Bicarbonate induced an albumin-independent change in lipid architecture that was detectable by an increase in merocyanine staining (due to protein kinase A-mediated phospholipid scrambling). The response was limited to a subpopulation of viable sperm cells that were sorted from the non-responding subpopulation by flow cytometry. The responding cells had reduced cholesterol levels (30% reduction) compared with non-responding cells. The subpopulation differences were caused by variable efficiencies in epididymal maturation as judged by cell morphology. Membrane cholesterol organization was observed with filipin, which labeled the entire sperm surface of non-stimulated and non-responding cells, but labeled only the apical surface area of bicarbonate-responding cells. Addition of albumin caused cholesterol efflux, but only in bicarbonate-responding cells that exhibited virtually no filipin labeling in the sperm head area. Albumin had no effect on other lipid components, and no affinity for cholesterol in the absence of bicarbonate. Therefore, bicarbonate induces first a lateral redistribution in the low cholesterol containing spermatozoa, which in turn facilitates cholesterol extraction by albumin. A model is proposed in which phospholipid scrambling induces the formation of an apical membrane raft in the sperm head surface that enables albumin mediated efflux of cholesterol.
Key words: Sperm capacitation, Cholesterol efflux, Lipid polarity, Membrane raft, Gamete adhesion, Acrosome reaction, Fertilization
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  M. G. Buffone, S. V. Verstraeten, J. C. Calamera, and G. F. Doncel High Cholesterol Content and Decreased Membrane Fluidity in Human Spermatozoa Are Associated With Protein Tyrosine Phosphorylation and Functional Deficiencies J Androl, September 1, 2009; 30(5): 552 - 558. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Druart, J.-L. Gatti, S. Huet, J.-L. Dacheux, and P. Humblot Hypotonic resistance of boar spermatozoa: sperm subpopulations and relationship with epididymal maturation and fertility Reproduction, February 1, 2009; 137(2): 205 - 213. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. R. Hunnicutt, D. E. Koppel, S. Kwitny, and A. E. Cowan Cyclic 3',5'-AMP Causes ADAM1/ADAM2 to Rapidly Diffuse Within the Plasma Membrane of Guinea Pig Sperm Biol Reprod, November 1, 2008; 79(5): 999 - 1007. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M Lemoine, I Grasseau, J P Brillard, and E Blesbois A reappraisal of the factors involved in in vitro initiation of the acrosome reaction in chicken spermatozoa Reproduction, October 1, 2008; 136(4): 391 - 399. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. E. Vance Thematic Review Series: Glycerolipids. Phosphatidylserine and phosphatidylethanolamine in mammalian cells: two metabolically related aminophospholipids J. Lipid Res., July 1, 2008; 49(7): 1377 - 1387. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Girouard, G. Frenette, and R. Sullivan Seminal Plasma Proteins Regulate the Association of Lipids and Proteins Within Detergent-Resistant Membrane Domains of Bovine Spermatozoa Biol Reprod, May 1, 2008; 78(5): 921 - 931. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I M Aparicio, M J Bragado, M C Gil, M Garcia-Herreros, L Gonzalez-Fernandez, J A Tapia, and L J Garcia-Marin Porcine sperm motility is regulated by serine phosphorylation of the glycogen synthase kinase-3{alpha} Reproduction, September 1, 2007; 134(3): 435 - 444. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A M Petrunkina, D Waberski, A R Gunzel-Apel, and E Topfer-Petersen Determinants of sperm quality and fertility in domestic species Reproduction, July 1, 2007; 134(1): 3 - 17. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Selvaraj, D. E. Buttke, A. Asano, J. L. Mcelwee, C. A. Wolff, J. L. Nelson, A. V. Klaus, G. R. Hunnicutt, and A. J. Travis GM1 Dynamics as a Marker for Membrane Changes Associated With the Process of Capacitation in Murine and Bovine Spermatozoa J Androl, July 1, 2007; 28(4): 588 - 599. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-F. Lusignan, A. Bergeron, M.-H. Crete, C. Lazure, and P. Manjunath Induction of Epididymal Boar Sperm Capacitation by pB1 and BSP-A1/-A2 Proteins, Members of the BSP Protein Family Biol Reprod, March 1, 2007; 76(3): 424 - 432. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. C. N. Chiu, M.-K. Chung, R. Koistinen, H. Koistinen, M. Seppala, P.-C. Ho, E. H. Y. Ng, K.-F. Lee, and W. S. B. Yeung Glycodelin-A interacts with fucosyltransferase on human sperm plasma membrane to inhibit spermatozoa-zona pellucida binding J. Cell Sci., January 1, 2007; 120(1): 33 - 44. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Baumber and S. A. Meyers Changes in Membrane Lipid Order With Capacitation in Rhesus Macaque (Macaca mulatta) Spermatozoa J Androl, July 1, 2006; 27(4): 578 - 587. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. E. Buttke, J. L. Nelson, P. N. Schlegel, G. R. Hunnicutt, and A. J. Travis Visualization of GM1 with Cholera Toxin B in Live Epididymal Versus Ejaculated Bull, Mouse, and Human Spermatozoa Biol Reprod, May 1, 2006; 74(5): 889 - 895. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Satake, R. M. A. Elliott, P. F. Watson, and W. V. Holt Sperm selection and competition in pigs may be mediated by the differential motility activation and suppression of sperm subpopulations within the oviduct J. Exp. Biol., April 15, 2006; 209(8): 1560 - 1572. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R.A. van Gestel, I.A. Brewis, P.R. Ashton, J.B. Helms, J.F. Brouwers, and B.M. Gadella Capacitation-dependent concentration of lipid rafts in the apical ridge head area of porcine sperm cells Mol. Hum. Reprod., August 1, 2005; 11(8): 583 - 590. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. C. N. Chiu, M.-K. Chung, H.-Y. Tsang, R. Koistinen, H. Koistinen, M. Seppala, K.-F. Lee, and W. S.B. Yeung Glycodelin-S in Human Seminal Plasma Reduces Cholesterol Efflux and Inhibits Capacitation of Spermatozoa J. Biol. Chem., July 8, 2005; 280(27): 25580 - 25589. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.H. Yeung, J.P. Barfield, M. Anapolski, and T.G. Cooper Volume regulation of mature and immature spermatozoa in a primate model, and possible ion channels involved Hum. Reprod., November 1, 2004; 19(11): 2587 - 2593. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. S. Baker, M. Thomas, and C. D. Thaler Sperm Membrane Dynamics Assessed by Changes in Lectin Fluorescence Before and After Capacitation J Androl, September 1, 2004; 25(5): 744 - 751. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Muratori, I. Porazzi, M. Luconi, S. Marchiani, G. Forti, and E. Baldi Annexin V Binding and Merocyanine Staining Fail to Detect Human Sperm Capacitation J Androl, September 1, 2004; 25(5): 797 - 810. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Shadan, P. S. James, E. A. Howes, and R. Jones Cholesterol Efflux Alters Lipid Raft Stability and Distribution During Capacitation of Boar Spermatozoa Biol Reprod, July 1, 2004; 71(1): 253 - 265. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Frasch, P. M. Henson, K. Nagaosa, M. B. Fessler, N. Borregaard, and D. L. Bratton Phospholipid Flip-Flop and Phospholipid Scramblase 1 (PLSCR1) Co-localize to Uropod Rafts in Formylated Met-Leu-Phe-stimulated Neutrophils J. Biol. Chem., April 23, 2004; 279(17): 17625 - 17633. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Pomorski, J. C. M. Holthuis, A. Herrmann, and G. van Meer Tracking down lipid flippases and their biological functions J. Cell Sci., February 22, 2004; 117(6): 805 - 813. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Rodeheffer and B. D. Shur Sperm from {beta}1,4-galactosyltransferase I-null mice exhibit precocious capacitation Development, February 1, 2004; 131(3): 491 - 501. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.G. Buffone, G.F. Doncel, C.I. Marin Briggiler, M.H. Vazquez-Levin, and J.C. Calamera Human sperm subpopulations: relationship between functional quality and protein tyrosine phosphorylation Hum. Reprod., January 1, 2004; 19(1): 139 - 146. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Masson, V. Drouineaud, P. Moiroux, T. Gautier, G. Dautin, M. Schneider, J. Fruchart-najib, M. Jauhiainen, C. Ehnholm, P. Sagot, et al. Human seminal plasma displays significant phospholipid transfer activity due to the presence of active phospholipid transfer protein Mol. Hum. Reprod., August 1, 2003; 9(8): 457 - 464. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Riou, F. Guegnard, Y. Le Vern, and D. Kerboeuf Modulation of the multidrug resistance (MDR) system in the nematode Haemonchus contortus by changing cholesterol content: effects on resistance to anthelmintics J. Antimicrob. Chemother., August 1, 2003; 52(2): 180 - 187. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. P. Roberts, J. A. Wamstad, K. M. Ensrud, and D. W. Hamilton Inhibition of Capacitation-Associated Tyrosine Phosphorylation Signaling in Rat Sperm by Epididymal Protein Crisp-1 Biol Reprod, August 1, 2003; 69(2): 572 - 581. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Ambrosini, G. Zolese, M. Wozniak, D. Genga, M. Boscaro, F. Mantero, and G. Balercia Idiopathic infertility: susceptibility of spermatozoa to in-vitro capacitation, in the presence and the absence of palmitylethanolamide (a homologue of anandamide), is strongly correlated with membrane polarity studied by Laurdan fluorescence Mol. Hum. Reprod., July 1, 2003; 9(7): 381 - 388. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.J. de Vries, T. Wiedmer, P.J. Sims, and B.M. Gadella Caspase-Independent Exposure of Aminophospholipids and Tyrosine Phosphorylation in Bicarbonate Responsive Human Sperm Cells Biol Reprod, June 1, 2003; 68(6): 2122 - 2134. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Wennemuth, A. E. Carlson, A. J. Harper, and D. F. Babcock Bicarbonate actions on flagellar and Ca2+-channel responses: initial events in sperm activation Development, April 1, 2003; 130(7): 1317 - 1326. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B.M. Gadella and R.A.P. Harrison Capacitation Induces Cyclic Adenosine 3',5'-Monophosphate-Dependent, but Apoptosis-Unrelated, Exposure of Aminophospholipids at the Apical Head Plasma Membrane of Boar Sperm Cells Biol Reprod, July 1, 2002; 67(1): 340 - 350. [Abstract] [Full Text] [PDF]
|
|
