|
|
|
|
|
||
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 114, Issue 2 281-291, Copyright © 2001 by Company of Biologists
JOURNAL ARTICLES |
M Karbowski, JH Spodnik, M Teranishi, M Wozniak, Y Nishizawa, J Usukura and T Wakabayashi
Department of Cell Biology and Molecular Pathology, Nagoya University School of Medicine, Nagoya, Japan.
Distribution of mitochondria as well as other intracellular organelles in mammalian cells is regulated by interphase microtubules. Here, we demonstrate a role of microtubules in the mitochondrial biogenesis using various microtubule-active drugs and human osteosarcoma cell line 143B cells and rat liver-derived RL-34 cells. Depolymerization of microtubules by nocodazole or colchicine, as well as 2-methoxyestradiol, a natural estrogen metabolite, arrested asynchronously cultured cells in G(2)/M phase of cell cycle and at the same time inhibited the mitochondrial mass increase and mtDNA replication. These drugs also inhibited the mitochondrial mass increase in the cells that were synchronized in cell cycle, which should occur during G(1) to G(2) phase progression in normal conditions. However, stabilization of microtubules by taxol did not affect the proliferation of mitochondria during the cell cycle, yet a prolonged incubation of cells with taxol induced an abnormal accumulation of mitochondria in cells arrested in G(2)/M phase of cell cycle. Taxol-induced accumulation of mitochondria was not only demonstrated by mitochondria-specific fluorescent dyes but also evidenced by the examination of cells transfected with yellow fluorescent protein fused with mitochondrial targeting sequence from subunit VIII of human cytochrome c oxidase (pEYFP) and by enhanced mtDNA replication. Two subpopulations of mitochondria were detected in taxol-treated cells: mitochondria with high Delta(psi)(m), detectable either by Mito Tracker Red CMXRos or by Green FM, and those with low Delta(psi)(m), detectable only by Green FM. However, taxol-induced increases in the mitochondrial mass and in the level of acetylated (alpha)-tubulin were abrogated by a co-treatment with taxol and nocodazole or taxol and colchicine. These data strongly suggest that interphase microtubules may be essential for the regulation of mitochondrial biogenesis in mammalian cells.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  W. J. H. Koopman, S. Verkaart, H. J. Visch, S. van Emst-de Vries, L. G. J. Nijtmans, J. A. M. Smeitink, and P. H. G. M. Willems Human NADH:ubiquinone oxidoreductase deficiency: radical changes in mitochondrial morphology? Am J Physiol Cell Physiol, July 1, 2007; 293(1): C22 - C29. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Taguchi, N. Ishihara, A. Jofuku, T. Oka, and K. Mihara Mitotic Phosphorylation of Dynamin-related GTPase Drp1 Participates in Mitochondrial Fission J. Biol. Chem., April 13, 2007; 282(15): 11521 - 11529. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. C. Logan The mitochondrial compartment J. Exp. Bot., March 1, 2006; 57(6): 1225 - 1243. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Giege, L. J. Sweetlove, V. Cognat, and C. J. Leaver Coordination of Nuclear and Mitochondrial Genome Expression during Mitochondrial Biogenesis in Arabidopsis PLANT CELL, May 1, 2005; 17(5): 1497 - 1512. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Liu, A. Vo, and W. L. McKeehan Specificity of the Methylation-Suppressed A Isoform of Candidate Tumor Suppressor RASSF1 for Microtubule Hyperstabilization Is Determined by Cell Death Inducer C19ORF5 Cancer Res., March 1, 2005; 65(5): 1830 - 1838. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Ricker, Z. Chen, X. P. Yang, V. S. Pribluda, G. M. Swartz, and C. Van Waes 2-Methoxyestradiol Inhibits Hypoxia-Inducible Factor 1{alpha}, Tumor Growth, and Angiogenesis and Augments Paclitaxel Efficacy in Head and Neck Squamous Cell Carcinoma Clin. Cancer Res., December 15, 2004; 10(24): 8665 - 8673. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Varadi, L. I. Johnson-Cadwell, V. Cirulli, Y. Yoon, V. J. Allan, and G. A. Rutter Cytoplasmic dynein regulates the subcellular distribution of mitochondria by controlling the recruitment of the fission factor dynamin-related protein-1 J. Cell Sci., September 1, 2004; 117(19): 4389 - 4400. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Toy-Miou-Leong, C. L. Cortes, A. Beaudet, W. Rostene, and P. Forgez Receptor Trafficking via the Perinuclear Recycling Compartment Accompanied by Cell Division Is Necessary for Permanent Neurotensin Cell Sensitization and Leads to Chronic Mitogen-activated Protein Kinase Activation J. Biol. Chem., March 26, 2004; 279(13): 12636 - 12646. [Abstract] [Full Text] [PDF]
|
|
