spacer gif spacer gif spacer gif spacer gif spacer gif
 QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    


This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Vincent-Schneider, H.
Right arrow Articles by Bonnerot, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vincent-Schneider, H.
Right arrow Articles by Bonnerot, C.
Social Bookmarking
 Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Journal of Cell Science, Vol 114, Issue 2 323-334, Copyright © 2001 by Company of Biologists


JOURNAL ARTICLES

Secretory granules of mast cells accumulate mature and immature MHC class II molecules

H Vincent-Schneider, C Thery, D Mazzeo, D Tenza, G Raposo and C Bonnerot
INSERM, U. 520, Institut Curie,12, rue Lhomond, France. bonnerot@curie.fr

Bone marrow-derived mast cells as well as dendritic cells, macrophages and B lymphocytes express major histocompatibility complex (MHC) class II molecules. In mast cells, the majority of MHC class II molecules reside in intracellular cell type-specific compartments, secretory granules. To understand the molecular basis for the localisation of MHC class II molecules in secretory granules, MHC class II molecules were expressed, together with the invariant chain, in the mast cell line, RBL-2H3. Using electron and confocal microscopy, we observed that in RBL-2H3 cells, mature and immature class II molecules accumulate in secretory granules. Two particular features of class II transport accounted for this intracellular localization: first, a large fraction of newly synthesized MHC class II molecules remained associated with invariant chain fragments. This defect, resulting in a slower rate of MHC class II maturation, was ascribed to a low cathepsin S activity. Second, although a small fraction of class II dimers matured (i.e. became free of invariant chain), allowing their association with antigenic peptides, they were retained in secretory granules. As a consequence of this intracellular localization, cell surface expression of class II molecules was strongly increased by cell activation stimuli which induced the release of the contents of secretory granules. Our results suggest that antigen presentation, and thereby antigen specific T cell stimulation, are regulated in mast cells by stimuli which induce mast cell activation.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
Cancer Res.Home page
E. Gounaris, N. R. Blatner, K. Dennis, F. Magnusson, M. F. Gurish, T. B. Strom, P. Beckhove, F. Gounari, and K. Khazaie
T-Regulatory Cells Shift from a Protective Anti-Inflammatory to a Cancer-Promoting Proinflammatory Phenotype in Polyposis
Cancer Res., July 1, 2009; 69(13): 5490 - 5497.
[Abstract] [Full Text] [PDF]


Home page
FASEB J.Home page
A. Bunbury, I. Potolicchio, R. Maitra, and L. Santambrogio
Functional analysis of monocyte MHC class II compartments
FASEB J, January 1, 2009; 23(1): 164 - 171.
[Abstract] [Full Text] [PDF]


Home page
BloodHome page
Y. Haberman, I. Ziv, Y. Gorzalczany, K. Hirschberg, L. Mittleman, M. Fukuda, and R. Sagi-Eisenberg
Synaptotagmin (Syt) IX is an essential determinant for protein sorting to secretory granules in mast cells
Blood, April 15, 2007; 109(8): 3385 - 3392.
[Abstract] [Full Text] [PDF]


Home page
J. Immunol.Home page
K. Sendide, A.-E. Deghmane, D. Pechkovsky, Y. Av-Gay, A. Talal, and Z. Hmama
Mycobacterium bovis BCG Attenuates Surface Expression of Mature Class II Molecules through IL-10-Dependent Inhibition of Cathepsin S
J. Immunol., October 15, 2005; 175(8): 5324 - 5332.
[Abstract] [Full Text] [PDF]


Home page
Int ImmunolHome page
H. Vincent-Schneider, P. Stumptner-Cuvelette, D. Lankar, S. Pain, G. Raposo, P. Benaroch, and C. Bonnerot
Exosomes bearing HLA-DR1 molecules need dendritic cells to efficiently stimulate specific T cells
Int. Immunol., July 1, 2002; 14(7): 713 - 722.
[Abstract] [Full Text] [PDF]




© The Company of Biologists Ltd 2001